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Luohe, China

Li Z.M.,Luohe Medical College
Sheng li xue bao : [Acta physiologica Sinica] | Year: 2012

Human amylin (hAmylin) is co-released with insulin from pancreatic B-cells and the actions of this peptide on its target tissues maintain the cell excitability and glucose homeostasis. Inappropriate control of hAmylin secretion may result in human disease, particularly Alzheimer's disease (AD). It's unknown that which kind of receptor is activated by human amylin, leading to the neurotoxicity in neurons of brain. Nicotinic acetylcholine receptors (nAChRs) are known to play a critical role in a variety of nervous diseases. In the present study, we sought to determine the inter-relationships between these two receptors by examining the actions of hAmylin and nicotine on whole-cell currents and membrane potential in basal forebrain neurons. Whole cell patch-clamp recordings were performed on enzymatically dissociated neurons of the diagonal band of Broca (DBB), a cholinergic basal forebrain nucleus. The results showed that either hAmylin or nicotine individually caused a dose-dependent (1 nmol/L-20 μmol/L) membrane depolarization and an increase in firing frequency of DBB neurons. Application of AC253, an amylin receptor antagonist, blocked the excitatory effects of not only hAmylin but also nicotine; dihydro-β-erythroidine (DHβE), a nAChR antagonist, also blocked the effects of nicotine and hAmylin. These electrophysiological results suggest that hAmylin receptor and nAChRs on DBB neurons are coupled and may function in a co-operative manner to influence the excitability of DBB neurons. This finding is important for us to understand the cause and mechanisms of AD. Source


Fan Y.,Southwest University | Fan Y.,Luohe Medical College | Shi W.,Southwest University | Shi W.,Yangtze Normal University | And 2 more authors.
Journal of Materials Chemistry A | Year: 2014

Mesoporous material supported CoFe2O4 magnetic nanoparticles possess unique peroxidase/oxidase-like activity, and react with luminol to yield a novel chemiluminescence without the need of H 2O2. Their oxidase-like activity shows pH and support dependence, and could be reversibly controlled by their pH. This offers a new method for manipulating the enzyme-like activity of nanoparticles. © 2014 The Royal Society of Chemistry. Source


Leng X.-F.,Guangxi Medical University | Chen M.-W.,Guangxi Medical University | Xian L.,Guangxi Medical University | Dai L.,Guangxi Medical University | And 2 more authors.
Journal of Experimental and Clinical Cancer Research | Year: 2012

Background: The aim of this study was to investigate prognostic value of excision repair cross-complementing 1 (ERCC1), BCL2-associated athanogene (BAG-1), the breast and ovarian cancer susceptibility gene 1 (BRCA1), ribonucleotide reductase subunit M1 (RRM1) and class III -tubulin (TUBB3) in patients with non-small cell lung cancer (NSCLC) who received platinum- based adjuvant chemotherapy. Methods. Messenger RNA expressions of these genes were examined in 85 tumor tissues and 34 adjacent tissue samples using semi-quantitative RT-PCR. The expressions of these five genes were analyzed in relation to chemotherapy and progression-free survival (PFS) and overall survival (OS). Seventy-four patients were enrolled into chemotherapy. Results: Patients with ERCC1 or BAG-1 negative expression had a significantly longer PFS (P = 0.001 and P = 0.001) and OS (P = 0.001 and P = 0.001) than those with positive expression. Patients with negative ERCC1 and BAG-1 expression benefited more from platinum regimen (P = 0.001 and P = 0.002). Patients with BRCA1 negative expression might have a longer OS (P = 0.052), but not PFS (P = 0.088) than those with BRCA1 positive expression. A significant relationship was observed between the mRNA expression of ERCC1 and BAG-1 (P = 0.042). In multivariate analysis, ERCC1 and BAG-1 were significantly favorable factors for PFS (P = 0.018 and P = 0.017) and OS (P = 0.027 and P = 0.022). Conclusions: ERCC1 and BAG-1 are determinants of survival after surgical treatment of NSCLC, and its mRNA expression in tumor tissues could be used to predict the prognosis of NSCLC treated by platinum. © 2012 Leng et al; licensee BioMed Central Ltd. Source


Li P.-A.,Luohe Medical College
Acta Crystallographica Section E: Structure Reports Online | Year: 2012

In the title solvated complex, [Ni(C17H21N 3O)(NCS)2(CH3OH)]·CH3OH, the Ni 2+ ion is coordinated by one phenolate O, one imine N, and one amine N atom of the tridentate Schiff base ligand, two thio-cyanate N atoms and one methanol O atom, resulting in a distorted cis-NiO2N4 octa-hedral geometry. The chelate ring formed by the phenolate O and imine N atoms approximates to an envelope with the Ni atom as the flap, whereas the chelate ring formed by the two N atoms is twisted about the C - C bond. In the crystal, the components are linked by O - H⋯O, N - H⋯O, N - H⋯S, and O - H⋯S hydrogen bonds. © Pin-Ai Li 2012. Source


Cao J.,Zhengzhou University | Wang J.-S.,Luohe Medical College | Ren X.-H.,Zhengzhou University | Zang W.-D.,Zhengzhou University
Spinal Cord | Year: 2015

Objective:To investigate the signaling pathways after astrocytes were activated in neuropathic pain.Methods:Thirty-six Sprague Dawley (s.d.) rats were randomly divided into two groups (each group with 18 s.d. rats) including chronic constriction injury (CCI) of the sciatic nerve model group and sham operation group. Operation was perform ed on the right leg in all rats. The lumbar spin al cord (L4 and L5) was taken to make paraffin slices on the 1st day before operation and the 1st, 3rd, 7th, 14th and 28th day after operation in each group. Paraffin slices were labeled with p38 mitogen-activated protein kinase (p38MAPK) and c-Jun N-terminal kinase (JNK) by immunofluorescence staining, and then were co-labeled with hexaribonucleotide binding protein-3 (NeuN), glial fibrillary acidic protein (GFAP) and anti-integrin αM (CD11b) antibody (OX-42) to explore the associations of p38MAPK and JNK with nerve cells or glial cell.Results:Compared with sham group, the pain threshold was significantly decreased, and astrocyte-activated markers, GFAP and vimentin were significantly increased in CCI group. The mean fluorescence intensities of p38MAPK and JNK were increased in the right spinal dorsal horn of CCI group. The coexpression of JNK and GFAP was found in astrocytes of the spinal dorsal horn in CCI group.Conclusion:JNK signal transduction pathway is involved in the pain signaling transduction of astrocytes. © 2015 International Spinal Cord Society All rights reserved. Source

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