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Yang X.,Affiliated to Binzhou Medical College | Wang X.,Affiliated to Binzhou Medical College | Shen H.,Linyi Peoples Hospital | Deng R.,Lunan Pharmaceutical Group Corporation | Xue K.,Linyi Peoples Hospital
Cell Biochemistry and Biophysics | Year: 2015

Circulating miR-21 is upregulated in breast cancer. However, correlation of miR-21 expression with clinic pathologic characteristics remains questionable. In this study, we investigate whether combination of circulation miR-21 with circulating tumor cells (CTCs) marker (EpCAM, MUS1, HER2) could improve diagnostic specificity of metastatic breast cancer. Total 223 breast cancer patients were included. 89 % patients were associated with upregulation of miR-21 compared with health control. 20 % patients were detected for CTCs marker positive. For higher specificity purpose, triple marker positive samples were selected as true CTCs positive, which only occupied 59.5 % of total metastatic breast cancer patients. Specificity of detection of CTCs was 96.7 %. Furthermore, 59.5 % metastatic breast cancer patients were shown both abnormal miR-21 and true CTCs positive according to distribution of true CTCs positive and abnormal miR-21; Combination of miR-21 and CTCs was increased specificity of metastatic detection to 100 %. Our findings suggested that combination of miR-21 with CTCs marker could be used for better diagnosis of metastatic breast cancer in the future. © 2015, Springer Science+Business Media New York. Source


Sun C.,Jinan University | Sun C.,Lunan Pharmaceutical Group Corporation | Lai X.,Jinan University | Huang X.,Jinan University | Zeng Y.,Jinan University
Biological and Pharmaceutical Bulletin | Year: 2014

Ginsenoside Rg1 (Rg1), one of the active ingredients in Panax ginseng, has been known to regulate many cellular processes. The purpose of this study was to investigate the protective effects of Rg1 on apoptosis in mouse cultured astrocytes in vitro and a mouse model of cerebral ischemia and reperfusion in vivo. The cell apoptosis was measured by fluorescence microplate reader and xCELLigence system and the Ca2+ overload was recorded by confocal microscopy. The mitochondrial membrane potential and reactive oxygen species (ROS) were determined by flow cytometry. BALB/c mice were subjected to transient middle cerebral artery occlusion (MCAO) and randomly divided into four groups: Sham (sham-operated +0.9% saline), MCAO (MCAO+0.9% saline), Rg1-L (MCAO+Rg1 20 mg/kg) and Rg1-H (MCAO+Rg1 40 mg/kg). Neurological deficit scores, brain water content and infarct volume were evaluated at 24 h after reperfusion. The results showed that Rg1 significantly attenuated H2O2-induced apoptosis in astrocytes. Rg1 efficiently inhibited intracellular Ca2+ overload, loss of mitochondrial membrane potential, and ROS production in astrocytes. In vivo study, it was also observed that Rg1 markedly reduced the neurological deficit scores, brain edema, and infarct volume in the model mice. These results suggest that Rg1 possesses significant neuroprotective effects, which might be related to the prevention of astrocytes from apoptosis. © 2014 The Pharmaceutical Society of Japan. Source


Zhou Y.,Huazhong University of Science and Technology | Li J.,Huazhong University of Science and Technology | He X.,Huazhong University of Science and Technology | Jia M.,Huazhong University of Science and Technology | And 4 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2013

A sensitive, simple and rapid high-performance liquid chromatography coupled with positive ion electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for the simultaneous determination of amlodipine, atorvastatin and its metabolites ortho-hydroxy atorvastatin and para-hydroxy atorvastatin in human plasma. The analytes were extracted from human plasma through liquid-liquid extraction method. A mixture of methyl tert-butyl ether and ethyl acetate (50:50, v/v) was used as the extractant. The chromatographic separation was achieved on a CAPCELLPAK CR 1:4 (5. μm, 150. mm. ×. 2.0. mm i.d.) column within 6.0. min with the mobile phase consisted of acetonitrile and ammonium acetate buffer (20. mM) containing 0.3% formic acid (50:50, v/v). Data acquisition was carried out in multiple reaction monitoring (MRM) mode. The method was validated and was successfully applied to the bioequivalence study of combination tablets containing AM and AT with coadministered individual drugs in 50 healthy Chinese male volunteers. © 2013. Source


Sun Z.X.,Lunan Pharmaceutical Group Corporation
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To optimize the technological parameters of the purification process for total organic acid and chlorogenic acid in Herba Artemisiae scopariae with macroporous adsorption resin. Static and dynamic adsorption-desorption methods were adopted to choose the optimal type of resin. Then orthogonal design L9 (3(4)) and single factor experiment were used to select the optimum purification process conditions. The optimal purification process for total organic acid and chlorogenic acid with HPD200A macroporous adsorption resin were as follows: the diameter height ratio of the resin was 1:6, the sample concentration was 1 g/mL, the absorption velocity was 1 BV/h (1 BV represented one column volume), the ratio of sample to HPD200A macroporous adsorption resin was 1.5:1 (W/W), 3 BV of water was used as purificant and 2 BV of 90% ethanol was used as eluant. Under these conditions, the purity of total organic acid and chlorogenic acid was 588.74% and 567.89%, respectively. The established purification process for total organic acid and chlorogenic acid in Herba Artemisiae scopariae with HPD200A macroporous adsorption resin is simple and stable and can be used in industrial production. Source


Wang Z.,Lunan Pharmaceutical Group Corporation | Ma Q.,Lunan Pharmaceutical Group Corporation
Journal of Heterocyclic Chemistry | Year: 2015

A new simple and efficient one-pot synthesis of 2-fluoroalkyl substituted indoles from 2-aminobenzyl alcohols with fluorine-containing carboxylic acids in the presence of Ph3P, CCl4, and NEt3 is described. Various kinds of 2-fluoroalkyl substituted indole derivatives can be conveniently prepared. © 2014 HeteroCorporation. Source

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