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Luleburgaz, Turkey

Polat F.,Kocaeli University | Diler S.B.,Nigde University | Azazi I.,Luleburgaz State Hospital | oden A,Nigde State Hospital
Asian Pacific Journal of Cancer Prevention

The aim of the present study was to determine whether endothelial nitric oxide synthase (eNOS) gene polymorphisms play a role in development of bladder cancer in the Turkish population. The study was performed on 75 patients (64 men, 11 women) with bladder cancer and 143 healthy individuals (107 men, 36 women) with any kind of cancer history. Three eNOS gene polymorphisms (T-786C promoter region, G894T and intron 4 VNTR 4a/b) were determined with polymerase chain reaction and restriction fragment lenght polymorphism methods. In our study, GT and TT genotypes for eNOS G894T polymorphism were found to significantly vary among patients with bladder cancer and control group (OR: 0.185, CI: 0.078-0.439, p=0.0001 and OR: 0.324, CI: 0.106-0.990, p=0.026). Also, the frequency of the 894T allele was significantly higher in patients with bladder cancer (51%). No association was identified for eNOS T-786C and intron 4 VNTR 4a/b polymorphisms between patients with bladder cancer and control groups in our Turkish population. Source

Ercan S.,Luleburgaz State Hospital | Ozkan S.,Dr Lutfi Kirdar Kartal Education And Research Hospital | Yucel N.,Dr Lutfi Kirdar Kartal Education And Research Hospital | Orcun A.,Dr Lutfi Kirdar Kartal Education And Research Hospital
Journal of Maternal-Fetal and Neonatal Medicine

Objective: When D-dimer is used to evaluate suspected venous thromboembolism in pregnant patients, the reference interval of common population may cause misinterpretation. The present study aims to determine reference intervals of D-dimer in the three trimesters. Methods: Four-hundred sixteen pregnant women and 32 non-pregnant women were enrolled in this cross-sectional study. Reference group had comprised 123 pregnant in the first trimester (5-11 week), 164 pregnant in the second trimester (13-20 week) and 126 pregnant women in the third trimester (25-35 week). D-dimer levels were analyzed via immunoturbidimetric assay. Results: If the threshold of 0.50 mg/L for diagnosis of VTE is used, 4.8% of pregnant women in the second trimester and 23.8% of pregnant women in the third trimester would have D-dimer levels exceeding this cut-off value. Reference intervals of D-dimer were determined as 0.11-0.40 mg/L; 0.14-0.75 mg/L and 0.16-1.3 mg/L in first, second and third trimester, respectively. Conclusion: The established D-dimer reference intervals for each trimester of pregnancy are different from those used in common population. These reference intervals may assist clinicians in making accurate clinical decisions. Further studies are needed to establish new cut-off values for the D-dimer to rule out VTE in each trimester. © 2014 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted. Source

Objective: The hemolysis index (HI) is an objective, easy and inexpensive method for detection hemolysis. However, the clinical laboratories fully rely on the manufacturers of analytical systems for test-specific the HI thresholds at which the hemolysis significantly interferes with the analyte. In the present study, it was aimed to determine test-specific the HI thresholds for significant interference in hemolyzed specimens produced by shearing method that closely mimics actual hemolysis occurs during blood collection. Methods: Whole anticoagulated bloods obtained from 34 healthy volunteers were repeatedly passed through a blood collection needle to produce hemolysis. 29 routine biochemistry analytes were assayed on the Roche Cobas 6000 c501 analyzer. Results: The bias values determined for antistreptolysin- O, total bilirubin, chloride, C-reactive protein, gamma- glutamyltransferase, glucose, high-density lipoprotein, urea and uric acid did not achieve the allowable total error, even at the highest HI value (1550). In contrast to, HI thresholds for aspartate aminotransferase, direct bilirubin, lactate dehydrogenase, and potassium were observed as 50. Our data were generally in good agreement with what gave the list of test-specific HI thresholds by the manufacturer. However, some assays including magnesium, total protein, rheumatoid factor, and sodium had lower the HI thresholds than those of recommended by the manufacturer. Conclusion: We concluded that hemolysis is differently influence routine biochemistry tests and the HI can provide a data of which analyte is to be affected. The analyte-dependent rejection according to the HI thresholds may prevent prolongation of turnaround time for analyte unaffected by hemolysis.In addition, it was concluded that the HI thresholds might be different according to tolerable error limits selected to determine significant interference. Therefore, before the HI thresholds recommended by manufacturer is applied in laboratory, it should be noted the total allowable error limits used by manufacturer during determination of HI thresholds. © 2016, Turkish Biochemistry Society. All rights reserved. Source

Ercan S.,Luleburgaz State Hospital | Yucel N.,Dr Lutfi Kirdar Kartal Education And Research Hospital | Orcun A.,Dr Lutfi Kirdar Kartal Education And Research Hospital
Journal of Medical Biochemistry

Background: The subjects with impaired glucose tolerance have an increased risk for future type 2 diabetes (T2DM); however, a significant number of individuals who develop T2DM have normal glucose tolerance (NGT) at baseline. The study aims to compare glycated hemoglobin (HbA1C) and homeostasis model assessment (HOMA-IR) levels to 30, 60 and 90-min glucose levels in subjects with NGT. Methods: A 75-g oral glucose tolerance test (OGTT) at 0, 30, 60, 90 and 120-min was performed in 1118 subjects without known T2DM. Blood samples were also drawn for fasting insulin and HbA1C levels. Results: Forty percent of the subjects with NGT had increased post-challenge values above the determined optimal glucose levels (10.2, 10.3 and 8.9 mmol/L at 30, 60 and 90-min, respectively). Compared to the subjects with NGT whose glucose levels were below the determined optimal values at 30, 60 and 90-min, we found significantly elevated HbA1C and HOMA-IR levels in the subjects with NGT whose glucose levels were above the determined optimal values (p<0.001). Conclusions: We conclude that the subjects with NGT have different HbA1C and HOMA-IR levels considering glucose levels measured earlier than at 2-h during OGTT. Further well-designed prospective studies are needed to define the significance of 30-min, 60-min and 90-min glucose levels in the prediction of disease in subjects with T2DM. Source

Akil A.,Acibadem Bodrum Hospital | Api O.,Yeditepe University | Oten Can E.,Kanuni Sultan Suleyman Education and Research Hospital | Ozkan S.,Cekerek State Hospital | And 3 more authors.
Journal of Maternal-Fetal and Neonatal Medicine

Objective: To determine whether preeclampsia causes fetal cardiac cell damage by assessing umbilical artery NT-proBNP, cardiac troponin I and homocysteine.Methods: A cross-sectional study with 73 fetuses between 26 and 40 weeks of gestation was performed. Thirty-three healthy mothers fetuses were control group (Group I). While 12 mildly pre-eclamptic mothers fetuses constituted Group II, 28 fetuses of severe pre-eclamptic mothers were Group III.Results: Umbilical cord mean NT-proBNP levels of Group I, II and III are 520.8 ± 404.5 pg/ml; 664.2 ± 215.9 pg/ml; and 1932.8 ± 2979.5 pg/ml, respectively (p = 0.0001). The number of neonates with NT-proBNP > 500 pg/mL that indicates severe cardiac damage is higher in Group III (p = 0.001). The mean homocysteine levels are also statistically significantly higher in Group III. Cardiac troponin I levels are not different between the groups (p = 0.46).Conclusion: Increased NT-proBNP and homocysteine might not only indicate some degree of in-utero cardiac cell damage but also feto-placental endothelial injury in the fetuses of severe pre-eclamptic mothers. Our finding that shows no evidence of correlation between cardiac troponin I levels with cell damage and endothelial injury requires further research. © 2015 Taylor & Francis. Source

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