Shukurov R.R.,Ltd Liability Company PHARMAPARK |
Lobanova N.V.,Ltd Liability Company PHARMAPARK |
Savinova I.N.,Ltd Liability Company PHARMAPARK |
Vorobyova I.G.,Ltd Liability Company PHARMAPARK |
And 7 more authors.
Applied Biochemistry and Microbiology | Year: 2014
A stable cell line that is based on CHO cells and produces 100 mg per liter of culture medium of recombinant darbepoetin alpha with a target glycosylated isoforms effective yield of about 30% has been selected. The expression product of the cell line was characterized and compared to the originator (Aranesp, Amgen). It was shown that the obtained preparation contained all isoforms characteristic of the originator. The created cell line can be used for the development of industrial cultivation and a purification scheme for recombinant darbepoetin alpha production. © 2014, Pleiades Publishing, Inc.
Leonov V.S.,The State Research Institute for Genetics and Selection of Industrial Microorganisms |
Litvinova N.A.,The State Research Institute for Genetics and Selection of Industrial Microorganisms |
Gorozhanina E.V.,Ltd Liability Company Pharmapark |
Starodubceva L.I.,Ltd Liability Company Pharmapark
Russian Journal of Biopharmaceuticals | Year: 2014
The process of industrial producer strains cultivation of hybrid recombinant proteins of human papillomavirus types 6, 11 and heat shock protein HSP70 is developed.