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Khelifi L.,LRGB Ecole Nationale Superieure Agronomique | Zarouri B.,LRGB Ecole Nationale Superieure Agronomique | Amdoun R.,LRGB Ecole Nationale Superieure Agronomique | Harfi B.,LRGB Ecole Nationale Superieure Agronomique | And 2 more authors.
Advances in Environmental Biology | Year: 2011

This study focused on the production of hyoscyamine through biotechnology process which is commonly used to enhance production of secondary metabolites of interest. To this end, hairy roots of 3 species of Datura stramonium were obtained following genetic transformation of hypocotyls using A4 strain of Agrobacterium rhizogenes. The results showed differences between species studied rather than between the induced root lines. This variability affected both growth of hairy root and their alkaloid content. The B5 medium was favourable for the production of hyoscyamine (0.4 mg/20 ml). In addition, the elicited hairy roots with 1 to 2 g/l NaCl produced hyoscyamine 3 times more than the control. Furthermore, jasmonic acid has not shown a positive effect on the accumulation of hyoscyamine in this study. The permeabilization of the hairy roots with Tween 20 liberated at least 25% of hyoscyamine in the culture medium facilitating hyoscyamine extraction and maintaining alive hairy roots.

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