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Mefti M.,LRGB Ecole Nationale Superieure Agronomique | Bouzerzour H.,Ferhat Abbas University Setif | Francia E.,University of Modena and Reggio Emilia | Ulrici A.,University of Modena and Reggio Emilia | And 4 more authors.
Euphytica | Year: 2016

A prime objective for improving perennial grasses for Mediterranean environments is the identification and selection of genotypes well adapted to semi-arid conditions. This study was conducted at an experimental site in Algeria over three consecutive seasons, with the aim of evaluating the agronomic performance and genetic diversity of 13 cultivars of two species of perennial grasses: six cocksfoot (Dactylis glomerata L.) and seven tall fescue (Festuca arundinacea Schreb. = syn.Lolium arundinaceum (Schreb.) Darbysh.) cultivars. Variation was found in both species for dry-matter yield, sward recovery, heading date, water-use efficiency (WUE), and summer senescence. Tall fescue cultivars gave greater yield in spring, with earlier heading date and higher WUE than cocksfoot, with no differences for the other traits. Among the genotypes, ‘Flecha’ was the most promising, together with ‘E-542’, ‘Fraydo’ and ‘Centurion’, all of which are tall fescue cultivars. Principal component analysis indicated that persistence of grass cover is relatively independent from dry-matter yield and water-use efficiency. The level of genetic diversity within each species was assessed using AFLP markers, and the molecular variation was analysed together with agronomic trait variation, with identification of molecular markers potentially associated with the relevant traits. © 2016 Springer Science+Business Media Dordrecht

Khelifi L.,LRGB Ecole Nationale Superieure Agronomique | Zarouri B.,LRGB Ecole Nationale Superieure Agronomique | Amdoun R.,LRGB Ecole Nationale Superieure Agronomique | Harfi B.,LRGB Ecole Nationale Superieure Agronomique | And 2 more authors.
Advances in Environmental Biology | Year: 2011

This study focused on the production of hyoscyamine through biotechnology process which is commonly used to enhance production of secondary metabolites of interest. To this end, hairy roots of 3 species of Datura stramonium were obtained following genetic transformation of hypocotyls using A4 strain of Agrobacterium rhizogenes. The results showed differences between species studied rather than between the induced root lines. This variability affected both growth of hairy root and their alkaloid content. The B5 medium was favourable for the production of hyoscyamine (0.4 mg/20 ml). In addition, the elicited hairy roots with 1 to 2 g/l NaCl produced hyoscyamine 3 times more than the control. Furthermore, jasmonic acid has not shown a positive effect on the accumulation of hyoscyamine in this study. The permeabilization of the hairy roots with Tween 20 liberated at least 25% of hyoscyamine in the culture medium facilitating hyoscyamine extraction and maintaining alive hairy roots.

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