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Marappagounder D.,Lifeline Multispecialty Hospital | Somasundaram I.,Loyola College Nungambakkam | Janvikula R.S.,Lifeline Multispecialty Hospital | Dorairaj S.,Loyola College Nungambakkam
Cell Biology International | Year: 2012

Recent scientific explorations in search of novel sources for autologous transplantation transpired an alternative source of MSCs (mesenchymal stem cells) derived from omentum fat. The scarcity of experimental evidences probing into the biosafety concerns of omentum fat-derived MSC under prolonged culture conditions limits its applicability as an efficient tool in regenerative medicine. This study, thus, aims to optimize human omentum fat-derived MSC in four different media [DMEM (Dulbecco's modified Eagle's medium) LG (low glucose), DMEM KO (knock out), α-MEM (α-minimal essential media) and DMEM F12] in the facets of phenotypic characterization, growth kinetics, differentiation and karyotyping under prolonged culture. The cells exhibited a similarity in expression profile for the majority of markers with evidential variations in certain markers. The relevance of omentum fat-derived MSCs became evident from its triumphant differentiation potential and karyotypic stability substantiated even at later passage. The results obtained from growth curve and PDT (population doubling time) lead to optimization of appropriate media for omentum fat-derived stem cell research, thereby bringing omentum fat into the forefront of regenerative medicine. © 2012 Portland Press Limited.

Swarna J.,Loyola College Nungambakkam | Ravindhran R.,Loyola College Nungambakkam
Research Journal of Biotechnology | Year: 2012

Momordica charantia L. cultivated in India is widely used as vegetable as well as in ayurvedic and unani system of medicines for the treatment of many diseases. The fruit of the plant possesses various medicinal properties like antimicrobial, antihel-minthic, anticancerous, antimutagenic, antitumo urous, antifertility, antidiabetic and abortifacient activities. Valuable secondary metabolites are produced by Agrobacterium rhizogenes-mediated hairy root induction. Hairy root production in Momordica charantia to produce charantin, an important hypoglycaemic component, was attempted for the first time. Transformation frequency in leaf and cotyledon explants was found to be 37.5 % and 25 % respectively. Hairy roots (3.7 cm in length) were observed from co-cultivated leaf explants after a period of three weeks. Charantin was detected in hairy roots and compared with fruit and leaf samples by performing thin layer chromatography.

Indumathi S.,Loyola College Nungambakkam | Dhanasekaran M.,Lifeline Multispeciality Hospital Perungudi | Rajkumar J.S.,Lifeline Multispeciality Hospital Perungudi | Sudarsanam D.,Loyola College Nungambakkam
Cytotechnology | Year: 2013

The immense potency of nutritional components of human breast milk and importance of breastfeeding is known worldwide. Recent researches had identified stem cells as integral component of human breast milk. Nevertheless, there is little proof of evidence on the stem cell constituents of breast milk. It is imperative to explore the cellular constituents of human breast milk, including of stem cells, to open new avenue in child's development and regeneration. Thus, we aimed at identifying the cellular constituents of human breast milk by phenotypic characterisation of diverse cell surface markers of hematopoietic stem cells (CD 34, CD 133, CD 117), mesenchymal stem cells (CD 90, CD 105, CD 73), myoepithelial cells (CD 29, CD 44), Immune cells (CD 209, CD 86, CD 83, CD 14, CD 13, HLADR, CD 45), as well as cell adhesion molecules (CD 31, CD 54, CD 166, CD 106, CD 49d), and other markers (ABCG2, CD140b) using flowcytometry. We found a lower expression of CD 34 (13.07 ± 2.0 %), CD 90 (7.79 ± 0.8 %) and CD 73 (2.19 ± 0.41 %), indicating scanty hematopoietic and mesenchymal stem cell population in human breast milk. On contrary, myoepithelial progenitors, cell adhesion molecules, immune cells and growth factors were identified as the major constituents of breast milk. Overall, this study illuminates the benefits of breast feeding as breast milk encompasses heterogeneous cellular components that benefits child's growth, immunity and development. However, further research on these constituents of human breast milk will widen their applicability in treatment of neonatal disorders. © 2012 Springer Science+Business Media B.V.

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