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Gohel H.R.,Loyola Center for Research and Development | Ghosh S.K.,Loyola Center for Research and Development | Braganza V.J.,Loyola Center for Research and Development
International Journal of Renewable Energy Research | Year: 2013

Demand of alternative fuels is increasing day by day due to the present crisis of petroleum based fuels. Biodiesel is one of the most demanding alternative fuel, which is produced either by animal or plant based feedstock. Both types of feedstocks are facing problem of constant availability in sufficient quantity for prolonged time period. This problem could be solved, if a source having higher lipid content is found in sufficient quantity. One probable solution is to use microorganisms; especially oleaginous species, because of their higher lipid content and almost similar composition as plant/animal lipid. For this experiment, yeast was selected because of several reasons like easy availability, rapid growth rate, higher lipid accumulation capacity, capable to grow on a variety of media etc. In this experiment, yeast was adapted to accumulate maximum quantity of lipids by providing metabolic stress condition, which was later converted into biodiesel by acid transesterification. Elimination of lipid extraction step has made the process much faster and easier as compared to traditional methods. Presence of fatty acid methyl esters were confirmed by high performance thin layer chromatography (HPTLC) and gas chromatography (GC). Fatty acids composition was determined by gas chromatography (GC) by comparing with standards.


Gohel H.R.,Loyola Center For Research and Development | Gohel H.R.,St Xaviers College | Ghosh S.K.,Loyola Center For Research and Development | Braganza V.J.,Loyola Center For Research and Development | Braganza V.J.,St Xaviers College
International Journal of Renewable Energy Research | Year: 2013

In India and several other developing countries, small towns and village are using biogas for fulfillment of their daily household burning and electricity production. In biogas plant cattle dung isa key ingredient, along with other organic feedstocks. Cellulose containing feedstocks are one of themost commonly used and highly accepted substrate for methanogenesis. Degradation of cellulose under anaerobic conditions is a very slow process and results into lower production rate of gas. Therefore, an attempt was made to increase the rate of biogas production by providing partially digested cellulose rich feedstock to the fermentor vessel. Digestion was carried out by extracellular enzymes produced by thermophiles. The vessels were incubated at different temperatures to determine the effects of temperature on rate of methanogenesis. As a result of the study, it was found that fermentor vessels fed with partially digested cellulose and thermophiles have great potential for production of significantly higher quantity of biogas at 55° in half the incubation time as required by conventional method. Rate of gas production under such conditions was found more than double in many cases. Not only this, a direct relationship between the rate of cellulose degradation and production of biogas was also observed.

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