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Xiao Y.,Loudi Municipal Central Hospital of Hunan Province
Wei sheng yan jiu = Journal of hygiene research | Year: 2011

To investigate the reversion of P-gp mediated multidrug resistance of U-2OS/ADM cells with curcumin in vitro. With doxorubicin as induction drugs, human osteosarcoma cell line U-2OS was induced object, multi-drug resistant of human osteosarcoma cell line model (U-2OS/ADM) were established by high-dose method, The before and after reversal efficacy of curcumin for U-2OS/ADM cells to chemotherapeutic drugs were measured by MTT assay. The effects of curcumin on Rh-123 uptake and efflux were analyzed by flow cytometer. (1) MTT demonstrates curcumin (20 micromol/L) can increase the cytotoxicity of Adriamycin to U-2OS/ADM cells. (2) The result of FCM shows that Curcumin can increase the accumulation of Rh-123 and increase the cytotoxicity of Adriamycin to U-2OS/ADM cells in a dose-dependent manner. The reversal mechanism of curcumin was blocked the function of P-gp in U-2OS/ADM cellular membrane.

Li Q.-X.,Central South University | Ao X.,Central South University | Wen L.,Central South University | Zhong H.-J.,Central South University | And 13 more authors.
National Medical Journal of India | Year: 2012

Background. We used recombinant adeno-associated virus vector of adiponectin (AAV2/1-Acrp30) to study the effects of increased levels of adioponectin (by the administration of rAAV2/1-Acrp30) on arteriosclerosis, glucose and lipid metabolism in Goto-Kakizaki (GK) rats with arteriosclerosis. Methods. Thirty GK rats with arteriosclerosis were divided into 3 equal groups: control group 1, control group 2 and the rAAV2/1-Acrp30-administered group. Saline, virus vector or rAAV2/1-Acrp30 (1012 ng/ml) vector genomes administered to the rats in the corresponding group by intramuscular injection to the posterior limb by single administration, respectively. After 8 weeks, fasting blood glucose, 2-hour postprandial blood glucose, glycosylated haemoglobin, serum insulin, serum total cholesterol, triglycerides, high-density lipoprotein and low-density lipoprotein were measured in each group, and the ultrastructure of the aorta was seen by light and electron microscopy. Results. Compared with control groups 1 and 2, in the rAAV2/1-Acrp30 group, there was a decrease in urine volume, fasting blood glucose, 2-hour postprandial blood glucose, glycosylated haemoglobin, serum total cholesterol, triglycerides and low-density lipoprotein, and an increase in body weight and high-density lipoprotein (p<0.05), while the level of serum insulin was not changed (p>0.05). Ultrastructure studies of the aorta showed that aortosclerosis in the rAAV2/1-Acrp30-administered group was less, and fewer lipid droplet vacuoles were seen in the vascular endothelial cytoplasm. Also various cell organelles and internal elastic lamina were seen, and there was no formation of lipid droplet and foam cells in the cytoplasm of the media of the smooth muscle. Conclusion. Adiponectin could improve blood glucose and lipid parameters and decrease atherosclerosis in the aorta of GK rats. © The National Medical Journal of India 2012.

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