Foan C.C.,University of Nottingham Malaysia Campus |
Lee Y.W.,Lot L3 l 1 Enterprise |
Tan J.S.,Felda Agricultural Services Sdn Bhd |
Alwee S.S.R.S.,Felda Agricultural Services Sdn Bhd
Asia-Pacific Journal of Molecular Biology and Biotechnology | Year: 2012
One of the key approaches for disease prevention in oil palm is to develop a disease resistant variety; this can be achieved through tagging of resistance genes in oil palm. In this study, a total of three putative resistance gene homologues (RGHs) have been isolated from oil palm (Elaeis guineensis) using primers targeted at the conserved motifs of the nucleotide binding site (NBS) domain. Comparison of the isolated RGH sequences with the NCBI GenBank database showed that one of the homologues had high identity to the disease resistance like proteins in coconut (90%) and tomato (87%). Phylogenetic analysis indicated that the RGHs clustered with R genes found in Arabidopsis. The findings will be useful for studying of resistant genes in oil palm.