Shi H.,Longnan Hospital |
Che Y.,Longnan Hospital |
Bai L.,Longnan Hospital |
Zhang J.,Longnan Hospital |
And 2 more authors.
Experimental and Therapeutic Medicine | Year: 2017
Type 2 diabetes (T2D) is a complex disorder caused by the combined effects of genetic inheritance and environmental factors. The abnormal secretion of albumin via urine is the characteristic feature of a diabetic nephropathy (DN) patient. Moreover, the detection of this observable characteristic feature of DN is quite late. As a result the time, at which DN is observable, large extent of kidney damage has already occurred. Thus, this late observation significantly decreases the chances of efficient management of DN and associated outcomes. The current biomarker used to detect DN is microalbuminuria, the presence of albumin in the urine. However, the current biomarkers often lead to false negative results. The high mobility group box (HMGB)1 is an upcoming molecule being explored for its application in the management of DN. The present review enlightens the current status of HMGB1 in DN. © 2017, Spandidos Publications. All rights reserved.
Zhou F.,Hubei University of Medicine |
Zou C.,Hubei University of Medicine |
Zhang C.,Hubei University of Medicine |
Nie L.,Hubei University of Medicine |
And 2 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2017
No effective strategy other than surgery can treat medullary thyroid carcinoma (MTC). Notch1 expression can induce apoptosis of MTC cells. This study aimed to investigate the correlation between miR-34a and Notch1 in MTC, plus their role in apoptosis of MTC cells. qRT-PCR was firstly used to test expression profiles of miR-34a in MTC tissues. Bioinformatics and luciferase reporter gene were employed to determine targeted gene of miR-34a. TT cell line was transfected by miR-34a mimic. Western Blot was used to examine Notch1 expression, followed by flow cytometry for cell cycle and cell proliferation. qRT-PCR found significantly depressed miR-34a expression in MTC cells (P<0.05 compared to tumor adjacent tissues). Both bioinformatics analysis and luciferase reporter gene assay demonstrated Notch1 as potential target of miR-34a. MiR-34a mimic transfection into TT cells remarkably inhibited Notch1 expression level (P<0.05). Flow cytometry found that over-expression of miR-34a significantly decreased cell number at S phase (P<0.05), elongated cell cycle, decreased cell proliferation activity and elevated apoptosis level.MiR-34a was down-regulated in MTC cells. It can inhibit proliferation potency of MTC cells via targeted inhibition on Notch1 expression, thus facilitating cell apoptosis.
Sun B.-L.,Universities of Shandong |
Shen F.-P.,Universities of Shandong |
Shen F.-P.,Longnan Hospital |
Wu Q.-J.,Universities of Shandong |
And 10 more authors.
Frontiers in Bioscience - Elite | Year: 2010
Cerebral vasospasm is the primary cause of sequelae and poor clinical conditions of subarachnoid hemorrhage (SAH); therefore, it is imperative to relieve vasospasm and improve cerebral blood supply. Calcitonin gene-related peptide (CGRP) is a potent vasodilator that is normally released by trigeminal sensory fibers but depleted following SAH. We propose that intranasal application may be an effective way to deliver CGRP to the brain and ameliorate vasospasm after SAH. In this study, we intranasally applied CGRP to rats and induced SAH by double-injection of autologous blood into the cisterna magna. Compared to intravenous injection, intranasal delivery led to a 10-fold higher level of CGRP in the brain. Intranasal CGRP significantly ameliorated vasospasm, improved cerebral blood flow, and reduced cortical and endothelial cell death. Moreover, CGRP increased the levels of vascular endothelial growth factor and stimulated angiogenesis. Altogether, our data demonstrate that intranasal CGRP delivery is a promising method for moderating vasospasm and reducing the associated ischemic brain injury after SAH in rats, and suggest that it may be a potential approach in clinic.
Sui F.,Longnan Hospital |
Zhang D.,Daqing Youtian Gerneral Hospital Aqing |
Lam S.C.B.,University of Pennsylvania |
Zhao L.,Longnan Hospital |
And 3 more authors.
Journal of X-Ray Science and Technology | Year: 2011
Previous lumbar motion analyses suggest the usefulness of quantitatively characterizing spine motion. However, the application of such measurements is still limited by the lack of user-friendly automatic spine motion analysis systems. This paper describes an automatic analysis system to measure lumbar spine disorders that consists of a spine motion guidance device, an X-ray imaging modality to acquire digitized video fluoroscopy (DVF) sequences and an automated tracking module with a graphical user interface (GUI). DVF sequences of the lumbar spine are recorded during flexion-extension under a guidance device. The automatic tracking software utilizing a particle filter locates the vertebra-of-interest in every frame of the sequence, and the tracking result is displayed on the GUI. Kinematic parameters are also extracted from the tracking results for motion analysis. We observed that, in a bone model test, the maximum fiducial error was 3.7%, and the maximum repeatability error in translation and rotation was 1.2% and 2.6%, respectively. In our simulated DVF sequence study, the automatic tracking was not successful when the noise intensity was greater than 0.50. In a noisy situation, the maximal difference was 1.3 mm in translation and 1° in the rotation angle. The errors were calculated in translation (fiducial error: 2.4%, repeatability error: 0.5%) and in the rotation angle (fiducial error: 1.0%, repeatability error: 0.7%). However, the automatic tracking software could successfully track simulated sequences contaminated by noise at a density ≤ 0.5 with very high accuracy, providing good reliability and robustness. A clinical trial with 10 healthy subjects and 2 lumbar spondylolisthesis patients were enrolled in this study. The measurement with auto-tacking of DVF provided some information not seen in the conventional X-ray. The results proposed the potential use of the proposed system for clinical applications. © 2011 - IOS Press and the authors. All rights reserved.
Wang L.,Longnan Hospital |
Xiao R.,Jiamusi University |
Peng H.,Daqing Oil Field General Hospital
Chinese Journal of Tissue Engineering Research | Year: 2013
Background: Skeletal class III malocclusion is a complex malocclusion in clinic, which has a great harm to the development of teeth, maxilla and face. Currently, skeletal class I malocclusion is often explored in the self-ligating system, and there is yet no reports addressing self-ligating system for treatment of skeletal class III malocclusion. Objective: To analyze the results of X-ray lateral cephalometric radiographs and model measurement before and after Clippy self-ligating system treatment of mild and moderate class III malocclusion so as to determine the therapeutic effect of Clippy self-ligating system. Methods: Thirty-four patients with skeletal class III malocclusion were selected from Daqing Oil Field General Hospital Group, aged 11-15 years (averagely 12.8 years), including 21 males and 13 females. The angle of ANB (subspinale, Nasion, supramental) was -2.1° on average. All the patients were treated with Clippy self-ligating system, and maxillary expansion and rapid palatal expansion appliance and auxiliary rehabilitation measures were performed during the treatment. The average course of treatment was 23.7 months. X-ray lateral cephalometric radiographs were shot to measure the relevant data. Results and Conclusion: (1) After treatment, the teeth of the 34 patients arranged regularly, normal jet and bite was established, and the maxillary and madibuar first molar relationship reached a neutral or slightly mesial relation. (2) The ANB angle was increased by 1.56° and the anterposterior dysplasia indicator decreased by -2.22°. Malocclusion reverted from class III to class I. (3) The face type of patients was improved. (4) The horizontal width of the maxillary and mandibular dental arch was expanded, and the sagittal length of the maxillary adental arch was increased. The Clippy self-ligating system is able to successfully collect mild to moderate skeletal class III malocclusion of early permanent dentition with non-extraction treatment, improves soft tissue profile, expands the horizontal width of the maxillary and mandibular dental arch and increases the sagittal length of the maxillary arch.
Sun S.-X.,3Rd Peoples Hospital of Jiulongpo |
Zhu J.-S.,3rd Peoples Hospital of Jiulongpo |
Cong Q.-X.,Longnan Hospital
Journal of Practical Oncology | Year: 2010
Objective: To evaluate the clinical application of diffusion tensor magnetic resonance imaging (DTI) in the assessment of radiotherapy effects on metastatic brain tumors. Methods: Twenty-eight patiente with metastatic brain tumors (44 foci) were enrolled in the study. All patients received pan-brain radiotherapy and followed up for at least 6 months after treatment. Routine MRI examination and DTI were performed to measure ADC, FA value in-focus and periventricle alba of 32 foci before radiotherapy, immediately and 1, 3, 6 months after radiotherapy. Results: Compared with pre-radiotherapy levels, ADC value was significantly rising 1, 3, 6 months after radiotherapy; while FA value changed very little. Conclusion: ADC value measured by DTI may be a more sensitive indicator for pathological change in brain metastatic foci and early effect of radiotherapy than routine MRI examination.
Gao Y.-F.,Harbin Medical University |
Zhu X.-D.,Heilongjiang Province Hospital |
Shi D.-M.,Second Heilongjiang Province Hospital |
Jing Z.-C.,Tongji University |
And 6 more authors.
International Journal of Molecular Medicine | Year: 2010
Statins have recently come under evaluation for the treatment of pulmonary arterial hypertension (PAH). The aim of this study was to examine the effects of atorvastatin on the clinical manifestations and expression of p38, p27 and Jab1 using a rat PAH model. Ninety-six male Wistar rats were divided into control (receiving no surgical treatment), vehicle and treatment groups, among which the last two groups underwent left pneumonectomy and were then treated with monocrotaline (MCT, 60 mg/kg). Both control and vehicle groups subsequently received saline, and the treatment group received atorvastatin (20 mg/kg) by stomach catheter. Rats were sacrificed, and mean pulmonary arterial pressure (mPAP) and right ventricle hypertrophy index (RVHI) were measured. The expression of p38, p27, and Jab1 was evaluated by immunohistochemistry and Western blotting. At 28 days, mPAP and RVHI and expression levels of Jab1 and p38 in the vehicle group were significantly higher than those in the treatment and control groups. However, the expression of p27 was lowest in the vehicle group among the three groups. Atorvastatin reduced PAP and RVHI in the rat PAH model, decreased expression of p38 and Jab1 but increased expression of p27.
Liao Q.,Daqing Oilfield General Hospital |
Tang Y.,Longnan Hospital |
Chi R.-J.,Shenyang Medical College |
Chen X.-C.,Shenyang Medical College |
And 2 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
BACKGROUND: Bone morphogenetic protein (BMP) is a protein possesses potential activity, which can increase and enhance its activity when the bone issues are damaged, so it can be used to repair the bone defects when combined with carrier. However, there are few reports concerning it as gene therapy. OBJECTIVE: To construct recombinant retroviral vector expressing human BMP2 gene and to discuss its biological function in osteoblasts. METHODS: BMP2-specific primers were designed and synthesized according gene sequence of human BMP2 gene in Genbank then BMP2 gene was amplified by Hifi PCR, which was recombined with cloning vector pDNR-CMV homologously into pDNR-CMV-BMP2 plasmid identified by BMP2 PCR and enzyme digestion of SalI and EcoRI as well as gene sequencing; recombinant plasmid pDNR-CMV-BMP2 and retroviral plasmid pLP-LNCX were recombined homologously in loxP sites into pLP-LNCX-BMP2 plasmid transferred into packing cell line PT67 and the supernatant was collected to assay viral titre. Human osteoblast was infected with retrovirus, then the growth of cells were observed by MTT, and the expression of BMP2 protein was detected by Western blotting at 48 hours transfection RESULTS AND CONCLUSION: Digestion, BMP2 PCR and gene sequencing of pDNR-CMV-BMP2 were coincided with expected. After transfected with plasmid pLP-LNCX-BMP2, PT67 cells could be screened with G418 only to get stably integrated in BMP2, of whose supernanant viral titre amounted to 5×108 pfu/mL. MTT assay showed that there had no evident difference in cellular inhibition between the normal and retrovirus groups at 72 hours after transfection (P > 0.05); Western blotting showed that the BMP2 was strong expressed at 48 hours after transfection. It demonstrated that BMP2 gene was successful cloned and its retrovirus vector was constructed.
Zhao W.,Longnan Hospital
Chinese Journal of Biologicals | Year: 2012
In 2006, scientists obtained a kind of pluripotency cells, named as induced pluripotent stem cells (iPS cells) by importing four transcription factors (Oct4, Sox2, cMyc and Klf4) into differentiated mouse fibroblast cells. Differentiated cells can be reprogramed to pluripotent stem cells stably with this technology and used for treatment of diseases. In this way, the limitation of application of traditional embryonic stem cells by social factors, such as ethical and moral factors, may be avoided. Therefore, the development of the method promotes the progress in research on theory and clinical application of stem cells enormously. This paper reviews the methods for inducing reprograme of iPS cells developed in recent years.
Wang H.-X.,Longnan Hospital |
Zhao W.,Longnan Hospital |
Feng Y.-T.,Longnan Hospital |
Liu P.-F.,Longnan Hospital
Chinese Journal of Biologicals | Year: 2014
Kidney failure (KF) is a kind of common clinical kidney disease generally caused by kidney toxic substances, serious kidney ischemia and other factors, which is classified as acute kidney failure (AKF) and chronic kidney failure (CKF) according to the time course of onset. With the development of medical treatment technique, lots of diseases have been controlled effectively in recent years. However, no effective therapy modes have been created to kidney failure which keeps unimproved death rate and increased attack rate. At present, the curative effect of bone marrow derived mesenchymal stem cells (BMSCs) on KF has been reported by researchers. This paper reviews the progress in research on role of BMSCs in treatment of KF in recent years.