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Daqing, China

Zhao W.,Longnan Hospital
Chinese Journal of Biologicals | Year: 2012

In 2006, scientists obtained a kind of pluripotency cells, named as induced pluripotent stem cells (iPS cells) by importing four transcription factors (Oct4, Sox2, cMyc and Klf4) into differentiated mouse fibroblast cells. Differentiated cells can be reprogramed to pluripotent stem cells stably with this technology and used for treatment of diseases. In this way, the limitation of application of traditional embryonic stem cells by social factors, such as ethical and moral factors, may be avoided. Therefore, the development of the method promotes the progress in research on theory and clinical application of stem cells enormously. This paper reviews the methods for inducing reprograme of iPS cells developed in recent years. Source

Gao Y.-F.,Harbin Medical University | Zhu X.-D.,Heilongjiang Province Hospital | Shi D.-M.,Second Heilongjiang Province Hospital | Jing Z.-C.,Tongji University | And 6 more authors.
International Journal of Molecular Medicine | Year: 2010

Statins have recently come under evaluation for the treatment of pulmonary arterial hypertension (PAH). The aim of this study was to examine the effects of atorvastatin on the clinical manifestations and expression of p38, p27 and Jab1 using a rat PAH model. Ninety-six male Wistar rats were divided into control (receiving no surgical treatment), vehicle and treatment groups, among which the last two groups underwent left pneumonectomy and were then treated with monocrotaline (MCT, 60 mg/kg). Both control and vehicle groups subsequently received saline, and the treatment group received atorvastatin (20 mg/kg) by stomach catheter. Rats were sacrificed, and mean pulmonary arterial pressure (mPAP) and right ventricle hypertrophy index (RVHI) were measured. The expression of p38, p27, and Jab1 was evaluated by immunohistochemistry and Western blotting. At 28 days, mPAP and RVHI and expression levels of Jab1 and p38 in the vehicle group were significantly higher than those in the treatment and control groups. However, the expression of p27 was lowest in the vehicle group among the three groups. Atorvastatin reduced PAP and RVHI in the rat PAH model, decreased expression of p38 and Jab1 but increased expression of p27. Source

Sun S.-X.,3Rd Peoples Hospital of Jiulongpo | Zhu J.-S.,3Rd Peoples Hospital of Jiulongpo | Cong Q.-X.,Longnan Hospital
Journal of Practical Oncology | Year: 2010

Objective: To evaluate the clinical application of diffusion tensor magnetic resonance imaging (DTI) in the assessment of radiotherapy effects on metastatic brain tumors. Methods: Twenty-eight patiente with metastatic brain tumors (44 foci) were enrolled in the study. All patients received pan-brain radiotherapy and followed up for at least 6 months after treatment. Routine MRI examination and DTI were performed to measure ADC, FA value in-focus and periventricle alba of 32 foci before radiotherapy, immediately and 1, 3, 6 months after radiotherapy. Results: Compared with pre-radiotherapy levels, ADC value was significantly rising 1, 3, 6 months after radiotherapy; while FA value changed very little. Conclusion: ADC value measured by DTI may be a more sensitive indicator for pathological change in brain metastatic foci and early effect of radiotherapy than routine MRI examination. Source

Liao Q.,Daqing Oilfield General Hospital | Tang Y.,Longnan Hospital | Chi R.-J.,Shenyang Medical College | Chen X.-C.,Shenyang Medical College | And 2 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: Bone morphogenetic protein (BMP) is a protein possesses potential activity, which can increase and enhance its activity when the bone issues are damaged, so it can be used to repair the bone defects when combined with carrier. However, there are few reports concerning it as gene therapy. OBJECTIVE: To construct recombinant retroviral vector expressing human BMP2 gene and to discuss its biological function in osteoblasts. METHODS: BMP2-specific primers were designed and synthesized according gene sequence of human BMP2 gene in Genbank then BMP2 gene was amplified by Hifi PCR, which was recombined with cloning vector pDNR-CMV homologously into pDNR-CMV-BMP2 plasmid identified by BMP2 PCR and enzyme digestion of SalI and EcoRI as well as gene sequencing; recombinant plasmid pDNR-CMV-BMP2 and retroviral plasmid pLP-LNCX were recombined homologously in loxP sites into pLP-LNCX-BMP2 plasmid transferred into packing cell line PT67 and the supernatant was collected to assay viral titre. Human osteoblast was infected with retrovirus, then the growth of cells were observed by MTT, and the expression of BMP2 protein was detected by Western blotting at 48 hours transfection RESULTS AND CONCLUSION: Digestion, BMP2 PCR and gene sequencing of pDNR-CMV-BMP2 were coincided with expected. After transfected with plasmid pLP-LNCX-BMP2, PT67 cells could be screened with G418 only to get stably integrated in BMP2, of whose supernanant viral titre amounted to 5×108 pfu/mL. MTT assay showed that there had no evident difference in cellular inhibition between the normal and retrovirus groups at 72 hours after transfection (P > 0.05); Western blotting showed that the BMP2 was strong expressed at 48 hours after transfection. It demonstrated that BMP2 gene was successful cloned and its retrovirus vector was constructed. Source

Sui F.,Longnan Hospital | Zhang D.,Daqing Youtian Gerneral Hospital Aqing | Lam S.C.B.,University of Pennsylvania | Zhao L.,Longnan Hospital | And 3 more authors.
Journal of X-Ray Science and Technology | Year: 2011

Previous lumbar motion analyses suggest the usefulness of quantitatively characterizing spine motion. However, the application of such measurements is still limited by the lack of user-friendly automatic spine motion analysis systems. This paper describes an automatic analysis system to measure lumbar spine disorders that consists of a spine motion guidance device, an X-ray imaging modality to acquire digitized video fluoroscopy (DVF) sequences and an automated tracking module with a graphical user interface (GUI). DVF sequences of the lumbar spine are recorded during flexion-extension under a guidance device. The automatic tracking software utilizing a particle filter locates the vertebra-of-interest in every frame of the sequence, and the tracking result is displayed on the GUI. Kinematic parameters are also extracted from the tracking results for motion analysis. We observed that, in a bone model test, the maximum fiducial error was 3.7%, and the maximum repeatability error in translation and rotation was 1.2% and 2.6%, respectively. In our simulated DVF sequence study, the automatic tracking was not successful when the noise intensity was greater than 0.50. In a noisy situation, the maximal difference was 1.3 mm in translation and 1° in the rotation angle. The errors were calculated in translation (fiducial error: 2.4%, repeatability error: 0.5%) and in the rotation angle (fiducial error: 1.0%, repeatability error: 0.7%). However, the automatic tracking software could successfully track simulated sequences contaminated by noise at a density ≤ 0.5 with very high accuracy, providing good reliability and robustness. A clinical trial with 10 healthy subjects and 2 lumbar spondylolisthesis patients were enrolled in this study. The measurement with auto-tacking of DVF provided some information not seen in the conventional X-ray. The results proposed the potential use of the proposed system for clinical applications. © 2011 - IOS Press and the authors. All rights reserved. Source

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