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Hou Q.,Longgang District Central Hospital | Lin J.,Southern Medical University | Huang W.,Sun Yat Sen University | Li M.,Sun Yat Sen University | And 2 more authors.
PLoS ONE | Year: 2015

C1q/TNF-related protein-3 (CTRP3) is a novel adipokine with roles in multiple cellular processes. However, little is known about its function in prostate cells. This study investigated the effects and mechanisms of CTRP3 in prostate cells. We first generated and purified CTRP3 protein in HEK 293T cells. Proliferation of RWPE-1 prostate cells was evaluated by MTT analyses under treatment with different concentrations of CTRP3 for various exposure times. The results revealed maximum enhancement of proliferation with 10 μg/mL CTRP3 for 72 h. Cell apoptosis and cell cycle were determined by TUNEL staining and flow cytometry analysis. TUNEL assay showed decreased TUNEL-positive cells in RWPE-1 prostate cells treated with CTRP3, and flow cytometry showed significantly decreased apoptotic cells upon CTRP3 treatment (treated cells, 8.34±1.175 vs. controls, 20.163±0.35) (P < 0.01). Moreover, flow cytometry analysis also showed a significant decrease of cells in the G1 phase and an increase of cells in the S and G2 phase upon CTRP3 treatment (treated cells, 42.85±1.40 vs. control, 52.77±0.90; 28.41±0.57 vs. 23.49±1.13; 27.08±1.97 vs. 22.20±1.32, respectively) (all P < 0.05). Two-dimensional gel electrophoresis and mass spectrometry identified differentially expressed proteins, including cytokeratin-19, GLRX3 and DDAH1, which were upregulated in CTRP3 treated cells, and cytokeratin-17 and 14-3-3 sigma, which were downregulated. GLRX3, DDAH1 and 14-3-3 sigma were confirmed using western blot analysis. A PKC inhibitor, staurosporine, was used to inhibit PKC activity in CTRP3 treated RWPE-1 cells. Staurosporine completely abolished the CTRP3-induced increased phosphorylation of intracellular PKC substrates and CTRP3-stimulated effect by RWPE-1 cells. Our results provide the first evidence for a physiological role of the novel adipokine, CTRP3, in prostate cells. Our findings suggest that CTRP3 could improve proliferation and anti-apoptosis of prostate cells through protein kinase C signaling pathways. © 2015 Hou et al. Source


Gao Y.,Longgang District Central Hospital | Wu X.,Longgang District Peoples Hospital | Zhou L.,Longgang District Central Hospital | Jiang A.,Longgang District Central Hospital | And 3 more authors.
Applied Mechanics and Materials | Year: 2012

Objective: The aim of the study was to explore most effective way to control infection during implant treatment. Methods: The study was carried out in two implant operating surgeries (Surgery A, Surgery B), which were set up air disinfectant machine and ultraviolet disinfection respectively. For baseline measurements, the number of bacterials in the air of the two surgeries was counted at 5min, 15min, 30min, 45min and 60min before sanitizing. To compare sanitizing effect of static air disinfection, air samples from these surgeries were collected for bacterial cultivation after sanitizing for 5min, 15min, 30min, 45min and 60min, and the the sterilization rate was calculated. To compare dynamic air disinfection, the two surgeries were sterilized before implant surgery, and the air samples were collected at 5min, 15min, 30min, 45min, 60min after implant operation. Results: There were no significant differences between air disinfectant machine and ultraviolet disinfection in static air disinfection (p<0.05). However, air disinfectant machine was more effective in dynamic air disinfection. Conclusion: Air disinfectant machine is the most effective way for the prevention of nosocomial infections. Source


Ye B.,Guangdong Medical College | Lin Z.-Y.,Longgang District Central Hospital | Xie L.-L.,Longgang District Central Hospital | Sun L.,Longgang District Central Hospital | Gao Y.-B.,Longgang District Central Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014

Background: Currently a large number of animal experiments and clinical studies have confirmed that, both immediate implants and delayed implants achieve successful osseointegration, but whether immediate implants can reduce or prevent physiological bone resorption of extracted teeth crypt alveolar crest has been the focus of debates among scholars. Objective: To evaluate the recent changes of immediate implant bone in anterior maxilla by the use of cone-beam CT. Methods: Eighteen patients with 18 maxillary anterior teeth that can not be reserved were treated with immediate implantation, and were included in the study. On the surgery day, postoperative 6 months and 1 year, the patients were detected with cone-beam CT. The bone wall thickness at the lingual side of alveolar crest at 4 mm, 6 mm, 8 mm away from the implant shoulder was measured. The bone height at the lingual-buccal side of missing teeth alveolar crest was also recorded. Results And Conclusion: At 6 months after surgery, the bone height at the lingual-buccal side of missing teeth alveolar crest was (1.83±0.05) mm and (1.50±0.04) mm, the bone resorption at the lingual side of alveolar crest at 4 mm, 6 mm, 8 mm away from the implant shoulder was (1.72±0.30) mm, (1.65±0.26) mm, (1.55±0.25) mm, respectively. At 1 year, the bone height at the lingual-buccal side of missing teeth alveolar crest was (0.85± 0.04) mm and (0.78±0.05) mm, the bone resorption at the lingual side of alveolar crest at 4 mm, 6 mm, 8 mm away from the implant shoulder was (0.52±0.20) mm, (0.45±0.16) mm, (0.32±0.15) mm, respectively. Immediate implant may induce horizontal resorption at the lingual side bone wall, but has no impact on the osseointegration of the implant, and the bone resorption is stable 1 year later. Source

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