Molecular typing of Riemerella anatipestifer serotype 14, an emerging pathogen for ducks [Zur molekularen Typisierung von feldstämmen von Riemerella anatipestifer serotyp 14, einem Krankheitserreger mit zunehmender Bedeutung für Enten]
Philipp H.-C.,Lohmann Animal Health GmbH |
Taras D.,Lohmann Animal Health GmbH |
Liman M.,Anicon Labor GmbH |
Grosse-Herrenthey A.,Lohmann Animal Health GmbH |
And 2 more authors.
Archiv fur Geflugelkunde | Year: 2013
Riemerella anatipestifer (R. anatipestifer) causes economically important infections in waterfowl and turkeys. Autogenous vaccines are widely used in duck farming due to the high antigenic variablity of R. anatipestifer and the lack of commercial vaccines. Since a few years, we have isolated increasing numbers of R. anatipestifer serotype 14 from vaccinated and non-vaccinated ducks. To further investigate the epidemiology and to improve the efficacy of autogenous vaccines, we used 49 R. anatipestifer serotype 14 strains and two controls belonging to other serotypes for further typing by pulse-field gel electrophoresis (PFGE) and matrix- assisted laser desorption-ionisation time-of-flight mass spectrometry (MALDI-TOF). The strains were isolated between 2008 and 2012 from four different geographic regions, all from ducks with the exception of one isolate originating from a goose. Seven distinct PFGE-patterns forming three clusters were obtained. Group A1 contained all 4 isolates from one of the regions. Group A contained 32 isolates from northwest Germany detected from 2010 onwards suggesting a recent emergence of this clone. All isolates in the study were correctly identified as R. anatipestifer by MALDI-TOF MS. The serotype 14 strains grouped into 8 closely related clusters by using the standard MALDITOF MS software. The clusters were unrelated to the data obtained by PFGE indicating that the currently available standard procedures of MALDI-TOF MS are suitable for the species identification of R. anatipestifer, but not for further subtyping within a serogroup. However, both methods confirm that there is considerable variability between R. anatipestifer isolations even within the same serotype. The genotyping by PFGE provides epidemiological information and identifies potentially emerging clones of R. anatipestifer. Such results are of particular value for the production of efficacious autogenous vaccines. © Verlag Eugen Ulmer, Stuttgart.
Applicationof protected L-Carnitine in dairy cows during transition and high lactation period [Einsatz von pansengeschütztem L-Carnitin bei Milchkühen in der Vorbereitungs- und der Hochleistungsphase]
Scholz H.,Hochschule Anhalt |
Ahrens A.,Thuringer Tierseuchenkasse |
Menn F.,Lohmann Animal Health GmbH |
Heimendahl E.V.,Lohmann Animal Health GmbH
Zuchtungskunde | Year: 2014
262 dairy cows were fed either 0 or 10 g of a rumen protected carnitine product (containing 2 g Carnitine) per cow and day supplemented via TMR. Milk yield and ingredients were investigated in all cows. For the investigation of blood parameters 110 cows were selected. Camitin supplementation significantly decreased blood NEFA concentration one week a.p. and there was a trend for decreased NEFA one week p.p. and 5 weeks p.p. GLDH in blood was significantly reduced one week a.p. and remained on a lower level throughout the trial in cows fed carnitine. Cholesterol level in blood was significantly decreased one week p.p. in heifers supplemented with carnitine. Cows in the supplemented group had also lower insemination index and improved conception rate. Carnitine supplementation led to an improved metabolic status of dairy cows during transition and high lactation period and increased fertility. Carnitine, in particular in a rumen protected variation can support metabolic health of dairy cows during the critical period of transition and high lactation. © Verlag Eugen Ulmer, Stuttgart.
Lardinois A.,Avian Virology and Immunology Unit |
Steensels M.,Avian Virology and Immunology Unit |
Lambrecht B.,Avian Virology and Immunology Unit |
Desloges N.,Lohmann Animal Health GmbH |
And 3 more authors.
Avian Diseases | Year: 2012
For the past decade, several recombinant Newcastle disease viruses (rNDV) have been used as a vector to express native or modified avian influenza (AI) hemagglutinins (HA) in order to give preventive protection against highly pathogenic avian influenza (HPAI) H5N1 viruses. Obtained protections were dependent on the age of the chickens, on the constructs and, in particular, on the homology between the HA that was inserted and the challenge strains. The objective of this study was to investigate the vaccine efficacy of a recombinant NDV La Sota-vectored vaccine expressing an Asian clade 1 H5 ectodomain (rNDV-H5) vaccine expressing a modified H5 ectodomain from an HPAI clade 1 H5N1 isolate as vaccine for 1-day-old specific-pathogen-free chickens. The inoculation route (oculonasal vs. drinking water), the dose-effect, and the protective range of this rNDV-H5 vaccine were studied. Both routes of vaccination induced an H5 serologic response and afforded a high degree of clinical protection against an Asian clade 1 HPAI H5N1 (AsH5N1) challenge without a significant difference between inoculation routes. A clear dose-effect could be demonstrated. Furthermore, when evaluating the protective range against antigenically divergent descendants of the Asian clade 1 HPAI H5N1 lineage, namely two Egyptian clade 2.2.1 H5N1 strains, the vaccine efficacy was less satisfactory. The rNDV-H5 vaccine provided good clinical protection and reduced viral shedding against Egyptian 2007 challenge but was unable to provide a similar protection against the more antigenically divergent Egyptian 2008 strain.
Hoffmann G.,Leibniz Institute for Agricultural Engineering |
Ammon C.,Leibniz Institute for Agricultural Engineering |
Volkamer L.,Leibniz Institute for Agricultural Engineering |
Surie C.,University of Veterinary Medicine Hannover |
Radko D.,Lohmann Animal Health GmbH
British Poultry Science | Year: 2013
The aim of this study was to assess the suitability of a dielectric measurement to evaluate the prevalence and severity of foot pad dermatitis (FPD) in broiler chickens.2. The study focussed on surveying the occurrence of FPD during the growing period of one broiler chicken flock. A scoring system consisting of 5 categories was used to assess the prevalence and severity of FPD macroscopically. Additionally, the dielectric constant (DC) of both foot pads of 50 chickens was measured in a triple iteration with the MoistureMeter D (Delfin Technologies, Kuopio, Finland) on three different dates.3. On all measurement days, DC and FPD score were negatively correlated. The severity of FPD increased during the growing period, and severe lesions occurred at an early stage.4. Further research is necessary to develop an effective early warning system for FPD in poultry houses. However, the non-invasive measurement of DC provides a reliable method for the objective assessment of occurrence and severity of FPD. © 2013 © British Poultry Science Ltd.
Kopp C.,University of Bonn |
Singh S.P.,University of Bonn |
Regenhard P.,University of Bonn |
Regenhard P.,Lohmann Animal Health GmbH |
And 4 more authors.
International Journal of Molecular Sciences | Year: 2014
Adiponectin and intracellular 5'adenosine monophosphate-activated protein kinase (AMPK) are important modulators of glucose and fat metabolism. Cinnamon exerts beneficial effects by improving insulin sensitivity and blood lipids, e.g., through increasing adiponectin concentrations and AMPK activation. The underlying mechanism is unknown. The Gi/Go-protein-coupled receptor (GPR) 109A stimulates adiponectin secretion after binding its ligand niacin. Trans-cinnamic acid (tCA), a compound of cinnamon is another ligand. We hypothesize whether AMPK activation and adiponectin secretion by tCA is transmitted by GPR signaling. Differentiated 3T3-L1 cells were incubated with pertussis toxin (PTX), an inhibitor of Gi/Go-protein-coupling, and treated with different tCA concentrations. Treatment with tCA increased adiponectin and the pAMPK/AMPK ratio (p ≤ 0.001). PTX incubation abolished the increased pAMPK/AMPK ratio and adiponectin secretion. The latter remained increased compared to controls (p ≤ 0.002). tCA treatment stimulated adiponectin secretion and AMPK activation; the inhibitory effect of PTX suggests GPR is involved in tCA stimulated signaling. © 2014 by the authors; licensee MDPI, Basel, Switzerland.
Lardinois A.,Veterinary and Agrochemical Research Center |
Vandersleyen O.,Veterinary and Agrochemical Research Center |
Steensels M.,Veterinary and Agrochemical Research Center |
Desloges N.,Lohmann Animal Health GmbH |
And 3 more authors.
Avian Diseases | Year: 2016
Maternally derived antibodies (MDA) are known to provide early protection from disease but also to interfere with vaccination efficacy of young chicks. This interference phenomenon is well described in the literature for viral diseases such as infectious bursal disease, Newcastle disease (ND), and avian influenza (AI). The goal of this work was to investigate the impact of H5 MDA and/or ND virus (NDV) MDA on the vaccine efficacy of a recombinant NDV-H5-vectored vaccine (rNDV-H5) against two antigenically divergent highly pathogenic AI (HPAI) H5N1 challenges. In chickens with both H5 and NDV MDA, a strong interference was observed with reduced clinical protection when compared to vaccinated specific-pathogen-free (SPF) chickens. In contrast, in chickens from commercial suppliers with NDV MDA only, a beneficial impact on the vaccine efficacy was observed with full protection and reduced viral excretion in comparison with rNDV-H5-vaccinated SPF chickens. To distinguish between the respective effects of the H5 and NDV MDA, an SPF model where passive immunity had been artificially induced by inoculations of H5 and NDV hyperimmunized polysera, respectively, was used. In the presence of H5 artificial MDA, a strong interference reflected by a reduction in vaccine protection was demonstrated whereas no interference and even an enhancing protective effect was confirmed in presence of NDV MDA. The present work suggests that H5 and NDV MDA interact differently with the rNDV-H5 vaccine with different consequences on its efficacy, the mechanisms of which require further investigations.
Nitrayova S.,Institute of Nutrition |
Windisch W.,TU Munich |
von Heimendahl E.,Lohmann Animal Health GmbH |
Muller A.,Lohmann Animal Health GmbH |
Bartelt J.,Lohmann Animal Health GmbH
Journal of Animal Science | Year: 2012
In contrast to inorganic Zn, organic Zn sources are absorbed via peptide or AA transport systems resulting in a higher digestibility and availability. Bioavailability of organically bound Zn seems also to be influenced by the type of complex being used. Fortytwo gilts (Large white × Landrace) with initial BW of 24 ± 1.4 kg were allotted to 6 treatments of 7 pigs each. Pigs were fed diets based on corn (Zea mays), barley (Hordeum vulgare), and soybean (Glycine max) meal containing either low or high Zn supplementation with ZnO, Zn-Met 1:2 complex, Zn-Gly, Zn proteinate (Zn-Prot), or Zn-yeast. Diets were fed during a 10-d adaptation followed by a 4-d quantitative collection. Daily feed allowance was restricted to 1400 g/pig. Pigs were weighed at the start and end of adaptation and collection and feed consumption was monitored daily. Dietary Zn addition was 10 and 100 mg/kg feed for ZnO and 10 mg/kg feed for other Zn sources. Corresponding ADG ranged from 437 to 587 g with the lowest (P < 0.05) ADG for 10 ppm ZnO. Only Zn-Met addition increased (P < 0.02) Zn digestibility and retention (P < 0.05). Organically bound Zn, in particular Zn from Zn- Met 1:2 and Zn-yeast, can replace higher dosages of ZnO due to better bioavailability indicating that type of chelate is important for Zn retention. Organically bound Zn may reduce Zn excretion, which consequently may lower the environmental impact. © 2012 American Society of Animal Science. All rights reserved.
Rahaus M.,Lohmann Animal Health GmbH |
Augustinski K.,Lohmann Animal Health GmbH |
Castells M.,Lohmann Animal Health GmbH |
Desloges N.,Lohmann Animal Health GmbH
Avian Diseases | Year: 2013
A possible interference after Marek's disease (MD) vaccination using an experimental bivalent vaccine, consisting of a redesigned CVI-988/Rispens-type MDV-1 strain and herpesvirus of turkeys, with vaccination against infectious bronchitis (IB) virus (IBV) or Newcastle disease (ND) virus (NDV) was examined. Day-old specific-pathogen-free chicks were concomitantly vaccinated with the bivalent MD vaccine (either intramuscularly or subcutaneously) and with commercially available vaccines against ND or IB. Afterward chickens were challenged with either lethal MD virus (MDV) or NDV strains or with a pathogenic IBV strain. After challenge, neither mortality nor notable clinical signs of MD, ND, or IB were observed in the vaccinated birds. The experimental bivalent MDV vaccine proved efficacious against lethal MDV challenge and did not affect the efficacy of the NDV or IBV vaccines. In conclusion, no signs of interference or adverse effects were detected. Thus, the vaccines can be administered concomitantly on chickens' first day of life. © American Association of Avian Pathologists.