Anyang, South Korea
Anyang, South Korea

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Park J.,Soongsil University | Park J.-H.,Soongsil University | Ock K.-S.,Soongsil University | Ganbold E.-O.,Soongsil University | And 4 more authors.
Journal of Colloid and Interface Science | Year: 2011

Intracellular uptake of serum-coated gold nanoparticles (AuNPs) in a single mammalian cell was examined in order to investigate the interactions of cell culture media and aromatic thiol-functionalized gold surfaces using micro-spectroscopic tools. The AuNPs modified by the aromatic thiols of para-aminobenzenethiol (ABT), para-hydroxy benzenethiol (HBT), and para-carboxylic benzenethiol (CBT, para-mercaptobenzoic acid) bearing NH 2, OH, and COOH surface functional groups are presumed to adsorb the serum proteins as indicated from the compiled quartz crystal microbalance (QCM) data. The QCM results indicate that among the constituents, fetal bovine serum (FBS) should be the major adsorbate species on AuNPs incubated in Roswell Park Memorial Institute (RPMI) medium. The functionalized AuNPs were found to be internalized as an aggregation state in mammalian cells as evidenced by transmission electron microscopy (TEM) images. We monitored such cellular uptake behaviors of aromatic thiol-modified AuNPs using dark-field microscopy (DFM)-guided confocal surface-enhanced Raman scattering techniques in order to identify the three-dimensional localization inside the single cell. We found that the uptake amounts of ABT, HBT, and CBT were similar by counting up to 70 particles inside the cells incubated in the solution mixture of the aromatic thiol and 1,4-phenylenediisocyanide (PDIC) as a reference. This result indicates for the short aromatic thiol compounds, the AuNPs should enter the cell after the serum-coating regardless of the surface functional groups. Considering that the aromatic thiols have little effect on the serum coating, the DFM/SERS method is an effective tool for monitoring the localization of AuNPs inside a single cell. © 2011 Elsevier Inc.


Seo J.H.,Soongsil University | Cho K.,Logos Biosystems Inc. | Lee S.Y.,Seoul National University | Joo S.-W.,Soongsil University
Nanotechnology | Year: 2011

Using live-cell imaging techniques we investigated concentration-dependent intracellular movements of fluorescence nanoparticles (NPs) in real-time after their entry into HeLa cells via incubation. Intracellular particle traces appeared to be a mixture of both random and fairly unidirectional movements of the particles. At rather low concentrations of NPs, a majority of the non-random intracellular particle trajectories are assumed to mostly go along microtubule networks after endocytosis, as evidenced from the inhibition test with nocodazole. On the other hand, as the concentrations of NPs increased, random motions were more frequently observed inside the cells. © 2011 IOP Publishing Ltd.


Kim M.,Soongsil University | Seo J.H.,Soongsil University | Jeon W.I.,Seoul National University | Kim M.-Y.,Soongsil University | And 3 more authors.
Talanta | Year: 2012

The anticancer drug doxorubicin (DOX) appeared to adsorb efficiently on TiO 2 nanoparticles (NPs) as evidenced by visible absorption and diffuse reflectance infrared spectroscopy data. The adsorbed drugs were found released in a controlled way by external glutathione (GSH). Fluorescence of DOX appeared to be quenched substantially by TiO 2 NPs. The fabrication and release of DOX on TiO 2 NPs were checked by monitoring the fluorescence. We could monitor real-time drug release in the live cell using fluorescence imaging techniques. By these methods, we were able to monitor up to a nanomolar amount of DOX release in vitro from TiO 2 NPs triggered by external GSH. In vivo fluorescence images of DOX were obtained from the subcutaneous site in living mice after GSH treatment. On the basis of label-free fluorescence quenching measurements, a real-time release of DOX on TiO 2 NPs can be monitored in vitro and in vivo after an external trigger of GSH. © 2011 Elsevier B.V. All rights reserved.


Lee M.,Chonbuk National University | Cho K.,Logos Biosystems Inc. | Yoon D.,South Korean National Institute of Animal Science | Yoo D.J.,Seonam University | Kang S.H.,Chonbuk National University
Electrophoresis | Year: 2010

A portable CE system was developed for the identification of cattle breeds. The system had a width of 44 cm, depth of 27 cm, height of 13 cm, and a weight of only ∼8 kg and included an LIF detector, with everything integrated into a small box. The specific sizes of genes were quickly separated and detected with a high sensitivity based on the difference in the DNA mobility using a diode-pumped solid-state LIF detector. Using this system, the 100-bp DNA ladder was analyzed under a 1.0% PVP (M r = 300 000) sieving gel matrix in a fused silica capillary with LODs of 4.4-13.0 pg/mL (S/N = 3) for 100-3000 bp DNAs, which indicates ten times improved value than other commercialized portable CE system. The migration times and the peak areas showed good reproducibilities with relative standard deviations that were less than 0.49 and 1.3% (n = 5), respectively. Based on the difference in the DNA mobility of the microsatellite and SNP markers, Korean cattle and Holstein were exactly identified as the model cattle breeds within 32 and 3.5 min, respectively. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.


Patent
Logos Biosystems Inc. | Date: 2014-06-25

Disclosed is a microchip. The microchip of the present invention is characterized by comprising: a first plate; and a second plate coupled to the first plate to form a channel, wherein the first plate comprises: a channel cover part; a first connection part spaced apart from the outer periphery of the channel cover part by a certain distance; and a tensile strength generation connecting part for mutually connecting the channel cover part and the first connection part so that the channel cover part elastically contacts the channel region formed on the second plate when the first plate is coupled to the second plate. According to the present invention, the channel cover part forming the channel elastically contacts the channel region formed on the second plate, thereby providing a microchip capable of providing a channel having a stable structure.


Patent
Logos Biosystems Inc. | Date: 2012-12-10

A cell counter includes: a sample slide configured to accommodate cells; a housing configured to be inserted into the inside of the sample slide from the outside of the sample slide; an object lens configured to be mounted within the housing, and to image-form a cell image for the cells projected from the sample slide; an image acquisition unit configured to be mounted within the housing together with the object lens, and to acquire the cell image image-formed by the object lens; and a first reflecting mirror provided between the sample slide and the object lens within the housing, and configured to change a projection direction of the cell image projected from the sample slide to the object lens.


Patent
Logos Biosystems Inc. | Date: 2011-11-18

Disclosed is a microchip. The microchip of the present invention is characterized by comprising: a first plate; and a second plate coupled to the first plate to form a channel, wherein the first plate comprises: a channel cover part; a first connection part spaced apart from the outer periphery of the channel cover part by a certain distance; and a tensile strength generation connecting part for mutually connecting the channel cover part and the first connection part so that the channel cover part elastically contacts the channel region formed on the second plate when the first plate is coupled to the second plate. According to the present invention, the channel cover part forming the channel elastically contacts the channel region formed on the second plate, thereby providing a microchip capable of providing a channel having a stable structure.


Patent
Logos Biosystems Inc. | Date: 2014-03-25

An excitation light from a first light source is adapted to be irradiated to a subject without passing through an objective lens so that the first light source and the subject may be arranged to be adjacent to each other. As a result, an excitation light having a high intensity of radiation may be irradiated to the subject to obtain a strong fluorescence signal. In addition, since the optical path of the excitation light from the first light source and the optical path of the fluorescent emission light emitted from the first dichroic mirror and the white light do not coincide with each other, a high S/N ratio may be obtained.


A method of deciding detachment time of cells, and a method and apparatus for subculturing the cells using the method. The method of deciding detachment time of the cells from a culturing vessel using a separation enzyme in subculturing the cells includes: a photographing step of photographing an image of the cells treated with the enzyme; a computing step of calculating a contrast value of the photographed image, and computing a change rate of the contrast value; a comparison step of repeating the photographing step and the computing step until an increasing change rate of the contrast value is reduced to a predetermined change rate or less; and a reporting step of reporting a time to remove the enzyme from the cells and to detach the cells from the culturing vessel when the increasing change rate of the contrast value is reduced to the predetermined change rate or less.


Patent
Logos Biosystems Inc. | Date: 2011-03-09

Disclosed is a microscope module. The microscope module includes an objective lens magnifying an image of a sample subject for observation; an observation part for observing the image of the sample magnified through the objective lens; and a reflective mirror provided between the objective lens and the sample such that the vertical distance between the sample and the objective lens is substantially reduced. Since the reflective lens is disposed between the objective lens and the observation part, the working distance from the sample to the objective lens is substantially reduced, so that the height of the microscope is remarkably reduced. Thus, the microscope may be used in a place with vertical spatial limitations.

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