Ling Y.,Anhui Agricultural University |
Ling Y.,Local Animal Genetic Resources Conservation and Biobreeding Laboratory |
Wang L.,Anhui Agricultural University |
Wang L.,Local Animal Genetic Resources Conservation and Biobreeding Laboratory |
And 11 more authors.
Journal of Animal and Veterinary Advances | Year: 2012
This study is about the correlation between COX II gene genetic variation and adult buck sperm motility traits, to provide genetics reference for the goat breeding. In this study, 120 adult male goat semen samples were collected from four populations. COXII gene polymorphism information was rapid screened using DNA pool and sequencing method. Each individual polymorphism of these goats was detected by RFLP. Sequencing results of DNA pool showed a single nucleotide mutation in COXII gene (A→G). The mutation caused a change of Hind III restriction site. RFLP results showed that the mutation associated with goat sperm traits. The vitality of fresh sperm and frozen sperm from BB-type goats was significantly higher than AA-type. This study provided foundation for establishment relationship between COX II gene SNP and goat sperm motility traits. It could be considered as a reference marker-assisted selection for buck semen quality traits and goat breeding. © Medwell Journals, 2012. Source