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Ling Y.,Anhui Agricultural University | Ling Y.,Local Animal Genetic Resources Conservation and Bio Breeding Laboratory of Anhui Province | Wang K.,Anhui Agricultural University | Wang K.,Local Animal Genetic Resources Conservation and Bio Breeding Laboratory of Anhui Province | And 6 more authors.
Journal of Animal and Plant Sciences | Year: 2015

The experiment was conducted to determine the genetic relationship between polymorphism of lipoprotein lipase(LPL) gene and intramuscular fat content in goat. 337 goats were collected from 6 goat populations including Anhui white goats, Boer goats, Linqu dairy goats, Minshan black fur goats, Jining grey goats and Matou goats. The genetic variations in exon 1,6, and 7 of LPL gene were detected by PCR-SSCP. Genetic effect of LPL gene on intramuscular fat content was analyzed by Anhui White and Boar populations. The results exhibited as follows: one polymorphism site was found in the exon1 of LPL and five genotypes were defined. Besides that, one polymorphism site was found in exon 6 and 7 of LPL gene, respectively, andthree genotypes AA, AB and BB were defined in both of exon 6 and 7. LPL gene increased intramuscular in the order of P1 (exon 1 of LPL): BB>AB>AA, P6 (exon 6 of LPL): AA>AB>BB, and P7 (exon 7 of LPL): BB>AB>AA. These results indicated that LPL gene could be considered as a candidate gene affecting intramuscular fat content in special goat populations. © 2015, Pakistan Agricultural Scientists Forum. All rights reserved. Source


Yin H.-Q.,Anhui Agricultural University | Cao H.-G.,Anhui Agricultural University | Cao H.-G.,Local Animal Genetic Resources Conservation and Bio Breeding Laboratory of Anhui Province | Sun X.-P.,Anhui Agricultural University | And 13 more authors.
Progress in Biochemistry and Biophysics | Year: 2010

In order to establish pig induced pluripotent stem cells (iPS) with defined factor fusion protein, four defined factors genes Oct4, Sox2, c-Myc and Klf4 were delivered into porcine fetal fibroblasts by lentiviral transfection. The porcine fetal fibroblasts expressed exogenous defined factor genes were sub-cultured, and the clear-cut cell clones were gradually isolated. The cell colones grew at similar rates and stability, exhibited normal karyotype, and expressed alkaline phosphatase, Oct4, Nanog and SSEA1. And these cells could differentiate into various kinds of tissue in teratomas. The results confirmed that the isolated cell clones were iPS cells. This would greatly facilitate the further improvement of the induction protocol and in-depth study and application of pig iPS cells. Source


Cao H.-G.,Anhui Agricultural University | Cao H.-G.,Local Animal Genetic Resources Conservation and Bio Breeding Laboratory of Anhui Province | Chen T.,Anhui Agricultural University | Yin H.-Q.,Anhui Agricultural University | And 15 more authors.
Progress in Biochemistry and Biophysics | Year: 2013

To investigate the effects of lentivirus on the growth and development of porcine fetal fibroblasts (PFBs), in this study PFBs were repeatedly infected by lentivirus. The results showed that lentivirus-mediated enhanced green fluorescent protein (EGFP) had stable and efficient expression in PFBs. Under the condition with leukemia inhibitory factor (LIF) and basic fibroblast growth factor (bFGF), some PFBs gradually changed their fibrous growth pattern into round cell morphology. These round cells proliferated and formed cell clones with clear edge boundary. Clones grew rapidly on feeder layers and passaged stably with normal karyotypes. These cells were positive for alkaline phosphatase (AP) and expressed stem cell markers Oct4, Nanog, and SSEA1. In addition, these cells formed embryoid bodies (EB) in vitro and three germ layers in vivo. After the cells were used as nuclear donors, the cleavage rate of the cloned embryos was 53.33%, the morula rate 9.03%, the blastocyst rate 2.07%, and the total cell number per hatched blastocyst was 26.5. Compared with embryos cloned from non- lentivirus PFBs, the morula rate and blastocyst rate were lower and significantly different (P < 0.05). Lentivirus can result in the generation of porcine iPS cells from PFBs, so it can be used as ideal material and tool for research such as epigenetic modification and cell reprogramming. Source

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