Bourassa D.,Georgia Institute of Technology |
Gleber S.-C.,Argonne National Laboratory |
Vogt S.,Argonne National Laboratory |
Yi H.,Emory University |
And 4 more authors.
Metallomics | Year: 2014
Synchrotron X-ray fluorescence (SXRF) microtomography has emerged as a powerful technique for the 3D visualization of the elemental distribution in biological samples. The mechanical stability, both of the instrument and the specimen, is paramount when acquiring tomographic projection series. By combining the progressive lowering of temperature method (PLT) with femtosecond laser sectioning, we were able to embed, excise, and preserve a zebrafish embryo at 24 hours post fertilization in an X-ray compatible, transparent resin for tomographic elemental imaging. Based on a data set comprised of 60 projections, acquired with a step size of 2 μm during 100 hours of beam time, we reconstructed the 3D distribution of zinc, iron, and copper using the iterative maximum likelihood expectation maximization (MLEM) reconstruction algorithm. The volumetric elemental maps, which entail over 124 million individual voxels for each transition metal, revealed distinct elemental distributions that could be correlated with characteristic anatomical features at this stage of embryonic development. © 2014 The Royal Society of Chemistry.
Richter H.,LLS Rowiak LaserLabSolutions GmbH |
Ojeda D.F.R.,LLS Rowiak LaserLabSolutions GmbH |
Will F.,LLS Rowiak LaserLabSolutions GmbH
BioSpektrum | Year: 2014
In tissue engineering multiple tasks have to be solved: from cell isolation for cultivation to histological sections of implants in animal models. However, it still lacks proper tools to section and image biomaterials and tissue for histology, 3D-culture or further biochemical analysis. This article describes the use of ultrashort pulse laser technology for sectioning for sample preparation. Integration of imaging technologies facilitates deep tissue imaging and guided cutting. © 2014, Springer-Verlag Berlin Heidelberg.