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Ahmadābād, India

Wagh J.,M. S. University of Baroda | Patel K.J.,Sardar Patel University | Soni P.,Sardar Patel University | Desai K.,Sardar Patel University | And 2 more authors.
Luminescence | Year: 2015

We describe the formulation of bovine serum albumin nanoparticles (BSA-NPs) by the coacervation method using surfactants. Plasmids (pUC18, pUC18egfp and pBBR1MCS-2) isolated from E. coli were incorporated into the BSA matrix by incubating in albumin solution prior to formulation of NPs. Plasmid incorporation was calculated by % yield, entrapment efficiency, DNA loading capacity and release of entrapped DNA by comparing with blank NPs. BSA-DNA binding studies were carried out by using fluorescence spectroscopy and Fourier Transform Infra Red Spectroscopy (FT-IR). The surface charge distribution of the NPs loaded with plasmid was calculated using zeta potential. The photoluminescence of BSA-NPs was quenched when loaded with pDNA, confirming the interaction of DNA with BSA. Altogether, these results provide evidences for the excellent DNA carrying efficiency of BSA-NPs without loss of plasmid's integrity. The NPs were used to transfect E. coli DH5α strain lacking ampicillin resistance. They, however, showed ampicillin resistance subsequent to transfection with plasmid encoding ampicillin resistance gene. Effect of transfection was confirmed by confocal microscopy and by the isolation of the plasmid by agarose gel electrophoresis from the transfected bacterial culture. This study clearly demonstrates the efficacy of BSA-NPs as delivery vehicle for pDNA transfection. © 2014 John Wiley & Sons, Ltd. Source

Patel M.G.,Parul Institute of Pharmacy | Pundarikakshudu K.,LJ Institute of Pharmacy
Indian Journal of Pharmacology | Year: 2016

Objectives: To study the anti-arthritic activity of Pathyadya Churna ethanol extract (PCE) in rats. Materials and Methods: Formaldehyde (2% v/v) or complete Freund's adjuvant (CFA 0.l mL) was injected in the left hind paw of male Wistar rats to develop arthritis. These rats were treated with three doses (135, 270, and 540 mg/kg) of PCE and one dose (10 mg/kg) of indomethacin. Anti-arthritic activity of the extract was assessed by noting paw volumes, rheumatoid factor (RF), blood parameters, and histological changes. Results: PCE treatment reduced paw swelling in arthritis caused by both formaldehyde and CFA. In CFA-treated rats, a significant decrease (P < 0.001) was seen in hemoglobin (13.92 g/dL to 9.97 g/dL), red blood cell count (7.32 million/mm3 to 6.58 million/mm3), and packed cell volume (44.04% to 30.56%). There were also significant (P < 0.001) elevations in white blood cell count (8220/-11,420/mm3), platelets (2.46-4.15 lakhs/mL), erythrocyte sedimentation rate (3.76-8.03/60 min), RF (7.17-26.77 IU/mL), triglycerides (71.69-96.60 mg/dL), total cholesterol (96.85-145.05 mg/dL), low-density lipoprotein (53.11-109.60 mg/dL), and very low-density lipoprotein (14.34-19.32 mg/dL). In CFA-induced arthritic rats, high-density lipoprotein decreased significantly (29.40 mg/dL to 16.13 mg/dL). Marked changes were noted in the histology of ankles. Treatment with PCE significantly reversed all these hematological and histological changes in a dose-dependent manner. Conclusions: PCE has a significant anti-arthritic activity in rats and is free from toxic effects. © 2016 Indian Journal of Pharmacology Published by Wolters Kluwer - Medknow. Source

Vyas V.K.,Nirma University | Ghate M.,Nirma University | Chintha C.,Nirma University | Patel P.,LJ Institute of Pharmacy
Current Computer-Aided Drug Design | Year: 2013

This study investigated 3D quantitative structure-activity relationships (QSAR) for a range of substituted benzimidazole derivatives as AngII-AT1 receptor antagonists by comparative molecular field analysis (CoMFA) and comparative molecular similarity indices (CoMSIA). The alignment strategy was used for these compounds by means of Distill function defined in SYBYL X 1.2. The best CoMFA and CoMSIA models were obtained for the training set compounds was statistically significant with leave-one-out (LOO) validation correlation coefficient (q2) of 0.613 and 0.622, cross validated coefficient (r2 cv) of 0.617 and 0.607, respectively and conventional coefficient (r2 ncv) of 0.886 and 0.859, respectively. Both the models were validated by a test set of 18 compounds giving satisfactory predicted correlation coefficient (r2 pred) of 0.714 and 0.549 for CoMFA and CoMSIA models, respectively. Generated 3D QSAR models were used for the prediction of pIC50 of an external dataset of 10 compounds for predictive validation, which gave conventional r2 of 0.893 for CoMFA model, and 0.774 for CoMSIA model. We identified some key features in substituted benzimidazole derivatives, such as the importance of lipophilicity and H-bonding at 2- and 5, 6, 7- position of benzimidazole ring, respectively, for good antagonistic activity. CoMFA and CoMSIA models generated in this work provide useful information for the design of new compounds and helped in prediction of antagonistic activity. © 2013 Bentham Science Publishers. Source

Khan M.Y.,Nirma University | Ali S.A.,Nirma University | Pundarikakshudu K.,LJ Institute of Pharmacy
Pharmaceutical Biology | Year: 2016

Context: Shorea robusta Gaertn.f. (Dipterocarpaceae) resin is used for treating infected wounds and burns by tribals in India.Objectives: The objective of this study was to investigate wound-healing activity of S. robusta resin extracts and essential oil in rats.Materials and methods: Methanol extract (SRME), petroleum ether, benzene insoluble fraction of methanol extract (SRPEBIME), and essential oil (SREO) of S. robusta resin were incorporated in soft yellow paraffin (10% w/w) and applied once daily on incision and excision wounds of Wistar rats. Framycetin ointment (1.0% w/w) was applied to the standard group. Tensile strength (on the 10th day), wound contraction, and scar area (on the 14th day) were recorded. On the 15th day, granulation tissues of excision wounds were analyzed for total protein, hydroxyproline, and hexosamine contents and activities of lipid peroxidation and super oxide dismutase (SOD). Histopathology of the wounds was also studied.Results and discussion: SRPEBIME and SREO healed incision and excision wounds faster than plain ointment base and framycetin. Tensile strength of SRPEBIME-treated incision wounds was 53% higher than that of control animals. In excision wounds, wound contraction and scar areas were found to be 99% and 7.7 mm2 (SRPEBIME) and 71.7% and 21 mm2 (control). Protein and hydroxyproline contents were higher in SRPEBIME (20.8 and 3.5% w/w) and SREO (17.4 and 2.8% w/w) groups as against 9.95 and 1.48% w/w in control groups. Histopathology revealed complete epithelization and new blood vessel formation in SRPEBIME groups.Discussion and conclusion: SRPEBIME and SREO have significant wound-healing activities on incision and excision wounds. © 2015 Informa Healthcare USA, Inc. Source

Senjalia H.K.,LJ Institute of Pharmacy | Shah S.,LJ Institute of Pharmacy
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014

The objective of the study was to develop and evaluate once daily sustained release tablet of Eprosartan mesylate involving dissolution enhancement approach for treating hypertentsion. For dissolution enhancement approach solid dispersion of Eprosartan mesylate in carrier PVP K30 at Drug: carrier ratio of 1: 0.25 was done by solvent evaporation method using methanol as solvent. Sustained release tablets were prepared by direct compression method using different polymers Ethocel 10FP, Eudragit RSPO and Eudragit RLPO. SR tablets containing Eprosartan mesylate were developed using different drug: polymer concentration. Evaluation of solid dispersion blend was done by FT-IR study, DSC and saturated solubility. FT-IR study revealed no chemical interaction between drug and polymers used. Pre compression parameters like angle of repose, bulk density, tapped density, Carr's index and Hausner's ratio were within the limits. Post compression parameters like hardness, thickness, friability, weight variation test and drug content complied with pharmacopoeial limit for the tablets. The result of in vitro dissolution studies indicated tablets containing blend of Eudragit RSPO and Ethocel 10FP (B11) has better sustained release action. To evaluate the effect of Eudragit RSPO and Ethocel 10FP, 32 factorial design was employed. The mechanism of drug release was found to follow First order kinetic model, because derived correlation coefficient 'r' (0.8555) and Korsmeyer-Peppas kinetic model suggesting that erosion is the predominant mechanism controlling the drug release. Stability study at 40°C±2°C/75 ± 5 % RH revealed that there was no significant change in disintegration time, drug content and % CDR after 30 days. Source

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