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Mahsud T.,University of Agriculture at Faisalabad | Jamil H.,University of Agriculture at Faisalabad | Qureshi Z.I.,University of Agriculture at Faisalabad | Asi M.N.,University of Agriculture at Faisalabad | And 3 more authors.
Small Ruminant Research | Year: 2013

The current study was conducted to determine the level of some major biochemical constituents in seminal plasma of Lohi rams, and its correlation with semen physical characteristic. For this purpose six mature healthy rams were selected from the sheep herd maintained at Livestock Production and Research Institute, Bahadurnagar, Okara. All the experimental rams were kept under similar feeding and management conditions. Total 48 semen samples were collected during the breeding season (mid-autumn). Immediately after the collection samples were subjected to quantitative and qualitative sperm parameters. Seminal plasma was separated by centrifugation at 3000 × g for 20. min and samples were stored at -20°C till the analysis. The mean concentration of Na, K, Mg, Cu, P, Zn, and total protein (TP) in seminal plasma was 222.90. mg/dl, 48.21. ppm, 10.77. mg/dl, 2.22. mg/dl, 10.26. mg/dl, 0.35. ppm and 1.87. mg/dl respectively. Statistical analysis of the data showed the significant negative correlation between biochemical constituent (Na, Mg, Cu) and sperm characteristics (ejaculated volume, mass activity, motility percentage, sperm concentration and spermatozoa abnormalities). There was significantly positive correlation between biochemical constituent (Zn, TP, P) and sperm characteristics (ejaculated volume, mass activity, motility percentage and sperm concentration). However they were negatively correlated with sperm abnormalities. © 2013 Elsevier B.V. Source


Shahzad W.,Livestock Production Research Institute Bahadurnagar | Aslam W.,Livestock Production Research Institute Bahadurnagar | Shakil M.,Livestock Production Research Institute Bahadurnagar | Munir R.,Veterinary Research Institute | And 4 more authors.
Pakistan Journal of Zoology | Year: 2012

A study was conducted to determine the prevalence of Mycoplasma capricolum subspecies capripneumoniae (Mccp) antibodies in beetal and beetal teddy cross breeds of goats in Okara, Sahiwal and Faisalabad districts by using field diagnostic Latex Agglutination Test (LAT) kit "CapriLAT" during September, 2010 to February, 2011. Out of 750 serum samples, 343 samples (45.70 %) were positive for Mccp antibodies. Prevalence of Mccp antibodies attributed to goat breeds significantly differs in Okara and Sahiwal districts as well as in the aggregate of three districts altogether. Copyright 2012 Zoological Society of Pakistan. Source


Shahzad W.,Livestock Production Research Institute Bahadurnagar | Munir R.,Veterinary Research Institute | Khan M.S.,University of Veterinary and Animal Sciences | Ahmad M.D.,University of Veterinary and Animal Sciences | And 3 more authors.
Tropical Animal Health and Production | Year: 2010

The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3. 5%) samples were positive by microscopy, while 97 (7. 7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies. © 2010 Springer Science+Business Media B.V. Source


Shahzad W.,Livestock Production Research Institute Bahadurnagar | Noor H.,University of Veterinary and Animal Sciences | Ud-Din Ahmad M.,University of Veterinary and Animal Sciences | Munir R.,Foot and Mouth Disease Research Center | And 5 more authors.
Iranian Journal of Parasitology | Year: 2013

Background: Babesia ovis and Theileria ovis are among the important and main etiological agents causing ovine babesiosis and ovine theileriosis, causing severe economic losses among sheep and goats. The aim of the present study was to determine the prevalence and molecular diagnosis of B. ovis and T. ovis in Lohi sheep at Livestock Experiment Station Bahadurnagar, Okara, Pakistan. Methods: The prevalence of B. ovis and T. ovis was investigated in 200 Lohi sheep of mixed age and sex by PCR during 2011. The assay was employed using primers Bbo-F & Bbo-R, specific for a 549-bp fragment in B. ovis genomic DNA and primers TSsr 170F & TSsr 670R, specific for a 520-bp fragment in T. ovis genomic DNA. The animals were also screened for both haemoparasites through stained thin blood smears. Results: Thirty two (16%), 48 (24%) and 26 (13%) were the number of animals found positive for B. ovis, T. ovis and for mixed infection with both parasites respectively, through microscopy. Sixty eight (34%), 73 (37%) and 42 (21%) were the number of animals found positive for B. ovis, T. ovis and for mixed infection with both parasites respectively, through PCR test. Conclusion: The results indicate the high sensitivity of PCR for surveying babesiosis and theileriosis and there is noteworthy prevalence of these diseases in sheep at an experimental station where environmental conditions are relatively controlled as compared to field conditions. Source


Shahzad W.,Livestock Production Research Institute Bahadurnagar | Munir R.,Veterinary Research Institute | Rana M.Y.,University of Veterinary and Animal Sciences | Ahmad R.,Livestock Production Research Institute Bahadurnagar | And 4 more authors.
Tropical Animal Health and Production | Year: 2013

Mycoplasma conjunctivae are etiological agents of infectious keratoconjunctivitis (IKC), commonly known as pink-eye in domestic sheep, goats and other wild animals in many parts of the world. A few young Lohi lambs maintained at Livestock Experiment Station (LES), Bahadurnagar, Okara, Pakistan showed clinical signs and symptoms of conjunctivitis, keratitis, severe lacrimation and varying degree of blindness. During January to March, 2011, a total of 36 ocular swabs were collected from IKC affected animals and were processed for isolation, identification, and characterization of M. conjunctivae. Sixteen (44. 44 %) out of 36 samples showed turbidity in PPLO broth. Twelve (75 %) out of 16 broth samples showed colony growth on PPLO agar. All 16 (44. 44 %) out of 36 turbid broth samples, 12 (75 %) out of 16 cultured on agar plate samples, and 21 (59 %) out of 36 sheep ocular direct swab samples were found positive for M. conjunctivae through polymerase chain reaction test by using M. conjunctivae-specific primer pair McoF1 and McoR1 and detecting a 750 base pair fragment on agarose gel. Topical application of 0. 5 % sterile solution of gentamycin (100 mg/ml) (Gentafar 10 %, FARVET, Netherlands) proved suitable for the treatment of IKC in Lohi lambs as all clinical signs of IKC disappeared after 5 days of treatment with this antibiotic. This is the first report about the prevalence, molecular diagnosis, and treatment of M. conjunctivae in Lohi sheep affected with infectious keratoconjunctivitis at LES, Bahadurnagar, Okara, Pakistan. © 2012 Springer Science+Business Media Dordrecht. Source

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