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Wang Q.,Sichuan University | Wang Q.,Chengdu University of Technology | Yu Q.,Sichuan University | Yu Q.,Liuzhou Vocational and Technical College | And 2 more authors.
Clinica Chimica Acta | Year: 2014

Human saliva, a multi-constituent oral fluid, has a high potential for early diagnosis of disease. Proteomic analysis of saliva holds promise as a non-invasive method that is advantageous over serum. This non-invasive diagnostic method represents developing trends in analytical and clinical chemistry. Significant technological advances in the field of proteomics during the last two decades have greatly facilitated the research toward this direction. However, these technologies still require integration and standardization of validation against accepted clinical and pathologic parameters. In this review, a summary of mass spectrometry-based technologies of saliva biomarker discovery, potential clinical applications, and challenges of saliva proteomics have been discussed, as well as latest technologies of validation and quantification of saliva biomarkers. It is likely that the use of saliva for early diagnostics of diseases will continue to expand thus providing a new approach of instrumental investigation for physiologic and physiological states. These novel biomarkers have obvious clinical utility that will help to diagnose many diseases at early stage. © 2014 Elsevier B.V.


Yang L.,Liuzhou Vocational and Technical College | Hua J.,Guilin University of Electronic Technology
Tezhong Zhuzao Ji Youse Hejin/Special Casting and Nonferrous Alloys | Year: 2016

The surface of AZ91 alloy engine cylinder was modified by surface plasma cladding. The micro-structure and phase constituent of AZ91 alloy substrate, TiB2-Al2O3 and varied Al: (TiB2-Al2O3) compound modified layer were analyzed, and the hardness, wear resistance and corrosion resistance of the modified layer were investigated. The results show that with the increase of distance from the surface of the modified layer, the micro-hardness of the modified layer is decreased gradually, however, the micro-hardness of the modified layer is the higher than that of AZ91 alloy substrate. The micro-hardness of the modified layer is decreased gradually with the increase of Al content, while the micro-hardness of TiB2-Al2O3 modified layer has the maximum value. With prolonging wear time, the wear weight loss of the substrate and modified layer are increased gradually, and the wear resistance modified layer with ω (Al): ω (TiB2-Al2O3) = 1:2 exhibits desirable. The corrosion resistance of the modified layer is improved compared with AZ91 alloy substrate, and the modified layer with ω (Al): ω (TiB2-Al2O3) = 1:2 has the satisfied corrosion resistance. © 2016, Journal Office of Special Casting and Nonferrous Alloys. All right reserved.


Yu Q.,University of Sichuan | Yu Q.,Liuzhou Vocational and Technical College | Zhan X.,University of Sichuan | Liu K.,University of Sichuan | And 3 more authors.
Analytical Chemistry | Year: 2013

In this study, antibody immobilization using a microwave-induced H 2O/Ar plasma pretreatment was achieved for the first time. Plasma was used to activate the surface of a capillary-based immunosensor by increasing the density of silicon hydroxyls and dangling bonds to ensure better silanization. The capture antibodies were covalently immobilized after the silanized surface reacted with glutaraldehyde and antibodies. A Cy3-labeled detection antibody was used in combination with the antigen captured by the immunosensor to complete the sandwich-type immunoassay, and the signals were measured using a laser-induced fluorescence system. Microwave-induced H 2O/Ar plasma pretreatment of the carcinoembryonic antigen (CEA) immunosensor improved the antibody immobilization, and there was an obvious improvement in the linear detection range, i.e., 1 order of magnitude compared with a commercial enzyme-linked immunosorbent assay (ELISA). This novel immobilization method dramatically improved the detection limit (0.5 pmol/L CEA) and sensitivity. Assay validation studies indicated that the correlation coefficient reached 0.9978, and the relative standard deviations were <7% for all samples, with recoveries of 99.7-107.1%. Furthermore, the immunosensor was applied successfully to CEA determination in actual saliva specimens with high sensitivity, acceptable precision, and reasonable accuracy. This enhanced CEA immunosensor based on microwave-induced H2O/Ar plasma was demonstrated to be a sensitive tool for CEA diagnostics. © 2013 American Chemical Society.


Yu Q.,University of Sichuan | Yu Q.,Liuzhou Vocational and Technical College | Wang Q.,University of Sichuan | Li B.,University of Sichuan | And 2 more authors.
Critical Reviews in Analytical Chemistry | Year: 2015

The high sensitivity of optical detection techniques and the highly specific reactions between antibodies and antigens mean that optical immunoassays have attracted much interest in the fields of protein, hormone, drug, and microorganism detection, without the need for complex separation and extraction steps. The immobilization of an antibody on a solid support is a crucial step for optical immunoassays. This review surveys the latest advances in current antibody immobilization techniques in detail, including physical adsorption, covalent attachment, bioaffinity immobilization, and some recently developed methods. Furthermore, specific consideration is given to oriented immobilization, which may improve the homogeneous surface covering the accessibility of the active site and surface coverage, and the analytical performance of immunoassays. Finally, new perspectives for antibody immobilization techniques in optical immunoassays are outlined. © 2015, Copyright © Taylor and Francis Group, LLC.


Yang L.,Liuzhou Vocational and Technical College
Jinshu Rechuli/Heat Treatment of Metals | Year: 2014

Taking the hot rolled Mg-Al-Sn alloy plate as the research object, the rolled samples were annealed at 250~450℃, the effects of grain size and strain rate on room temperature deformation behavior of Mg-Al-Sn alloy were analyzed. The results show that at the same strain, with grain size increasing, the yield strength, tensile strength and elongation of the alloy decrease, and the number of twins raise. With the increase of strain, the number of twins in the same grain size of the alloy increase gradually. With the strain rate decreasing, the yield strength and tensile strength of the alloy decrease, while the elongation increases. With the strain rate decreasing, the volume fraction of twins in the alloy decreases at the same strain.


Yang L.,Liuzhou Vocational and Technical College | Zou J.,Liuzhou Vocational and Technical College
Jinshu Rechuli/Heat Treatment of Metals | Year: 2014

Al-Mg-Si-Mn-Cr-Ti alloy ingots were prepared by semi continuous casting. Mechanical properties of the tested alloy treated with different heat treatment processes were tested by universal tensile testing machine. Microstructure of the alloy under different heat treatment state was analyzed by means of optical microscope and TEM. The effects of heat treatment processes on microstructure and mechanical properties of the Al-Mg-Si-Mn-Cr-Ti alloy were investigated. The results show that the mechanical properties of the Al-Mg-Si-Mn-Cr-Ti alloy after spray quenching is good, and the proper heat treatment process for the alloy is solid solution treated at 480 ℃ for 1 h and then aged at 175 ℃ for 10 h.


Li B.,University of Sichuan | Yu Q.,University of Sichuan | Yu Q.,Liuzhou Vocational and Technical College | Duan Y.,University of Sichuan
Critical Reviews in Biotechnology | Year: 2015

Infectious diseases caused by pathogens have become a life-threatening problem for millions of people around the world in recent years. Therefore, the need of efficient, fast, low-cost and user-friendly biosensing systems to monitor pathogen has increased enormously in the last few years. This paper presents an overview of different fluorescent labels and the utilization of fluorescence-based biosensor techniques for rapid, direct, sensitive and real-time identification of bacteria. In these biosensors, organic dyes, nanomaterials and rare-earth elements are playing an increasing role in the design of biosensing systems with an interest for applications in bacterial analysis. © 2015 Informa Healthcare USA, Inc.


Qu S.G.,South China University of Technology | Lou H.S.,South China University of Technology | Lou H.S.,Liuzhou Vocational and Technical College | Li X.Q.,South China University of Technology
Journal of Composite Materials | Year: 2015

SiC particles reinforced aluminum matrix composites (SiCp/Al) with SiC content of more than 60%, without particle segregation for mirror substrate applications, are prepared by using the improved process. The improved process contains sintering process design, preform quality traceability, and equipment designing. The maximum SiC volume fraction of 70.4% is obtained by optimizing the selection of the following proportion: 71.4% coarse, 7.1% medium, and 21.5% fine particles. The variation of SiC volume fraction is correlated with the percentage of fine particles by using regression analysis. The preforms with the compressive strength above 10.34 MPa are prepared by sintering at 1600°C for 2 h. Results showed that no defects in the scale of more than 10 μm are observed in the preforms. Due to the generation of a SiO2 layer during the sintering process, the formation of Al4C3 phase was inhibited. As the SiC particle volume fraction increased from 65.6% to 70.4%, the bending strength increased from 483.2 MPa to 585.2 MPa. The elastic modulus also increased from 174.2 GPa to 206.1 GPa, while the coefficient of thermal expansion between 50°C and 350°C decreased from 11.02 × 10-6/°C to 9.53 × 10-6/°C. © The Author(s) 2015.


Yu Q.,University of Sichuan | Yu Q.,Liuzhou Vocational and Technical College | Wang X.,University of Sichuan | Duan Y.,University of Sichuan
Analytical Chemistry | Year: 2014

A novel capillary-based three-dimensional (3D) fluoroimmunosensor for carcinoembryonic antigen detection was explored for the first time. The immunosensor was designed in symmetrical cylinder structure and fabricated with capillary tubes encapsulated in a quartz tube. The 3D design of the sensor increased the area of sensing surface, flexibility in light path design and efficiency of fluorescence collection by aluminum foil, resulting in analytical performance improvement. The CEA immunosensor was constructed in double antibody sandwich format. Fluorescence signals from DyLight 550-labeled antibody were measured using a laser-induced fluorescence spectrometry. There is an obvious improvement in the linear detection range of 0.7-80 ng/mL. This novel 3D immunosensor dramatically improved the detection limit (1.1 pmol/L CEA) and sensitivity. Assay validation studies indicated that the correlation coefficient reached 0.9935, with recoveries of 92.82-118.81%. Furthermore, the immunosensor was successfully applied to CEA determination in actual saliva specimens with high sensitivity and acceptable precision. Regarding accuracy, the results obtained by 3D immunosensor were not significantly different (t test) from those obtained by validated electrochemiluminescence immunoassay. This new 3D CEA immunosensor was demonstrated to be a high-performance tool for CEA diagnostics. © 2014 American Chemical Society.


Ji D.,Liuzhou Vocational and Technical College | Zhang H.F.,Liuzhou Vocational and Technical College | Yang J.,Liuzhou Wuling Automobile Industry Co.
Advanced Materials Research | Year: 2014

Based on design requirements of plug-in hybrid vehicle, using the method of theoretical analysis, the parameters matching design of drive motor, engine-generator set, battery, transmission and other subsystems is executed, and modeling and simulation are carried out using AVL-CRUISE software, simulation results show that the design is reasonable, laying a good foundation for the smooth development of the plug-in hybrid vehicle. © (2014) Trans Tech Publications, Switzerland.

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