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Shen S.,Liuzhou Hospital of Traditional Chinese Medicine | Wang J.,Guangxi University | Liang J.,Guangxi Medical University | He D.,Xian Jiaotong University
World Journal of Urology | Year: 2013

Purpose: Idiopathic asthenozoospermia is considered as one of the causes of male infertility and characterized by reduced sperm motility. For a better determination of pathogenic mechanism of asthenozoospermia, the exploration of differentially expressed proteins in normal sperm motility and idiopathic asthenozoospermia was conducted in our study. Methods: Sperm proteins were extracted and isolated by two-dimensional electrophoresis. All significantly changed protein spots were picked up from 2D gels and identified by tandem mass spectrometry. Sixteen of the thirty-three total differentially expressed protein spots were successfully identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry. Results: Sixteen proteins identified belonged to 15 unique protein groups. GRP78, lactoferrin, SPANXB, PGK2, flagellin, DJ-1, XPA binding protein 2, CAB2, GPX4, and GAPDH were the first to be identified as differentially expressed proteins in idiopathic asthenospermia patients. Meanwhile, the analysis of quantitative RT-PCR was carried out to compare the protein levels, and the results indicated that the expression levels of the gene and protein were not entirely consistent. Conclusions: These experimental results expand the scope of the protein database, generating targets for further investigation of the pathogenic mechanism of idiopathic asthenozoospermia. © 2013 Springer-Verlag Berlin Heidelberg. Source


Li X.-M.,Guangdong Medical University | Lu C.-J.,Liuzhou Hospital of Traditional Chinese Medicine | Zhou Y.-F.,Guangdong Medical University | Zhou Z.-K.,Guangdong Medical University | And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Long-term clinical research has demonstrated that Huangqi Sanxian decoction exerts a certain curative effect on osteoporosis. In addition, experimental studies have also showed that Huangqi Sanxian decoction can improve the biomechanical indexes in experimental animals. The present study aimed to investigate the effect of Huangqi Sanxian decoction on osteoblasts in the cellular level. OBJECTIVE: To observe the effects of Huangqi Sanxian decoction on gene regulation of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in osteoblasts cultured in vitro and to investigate the action mechanism underlying the curative effect of Huangqi Sanxian decoction on osteoporosis. METHODS: Huangqi Sanxian decoction, Xianlinggubao capsule containing serum and blank serum were prepared to intervening osteoblasts from newborn Sprague-Dawley rats cultured in vitro. Cellular morphology was observed, and western blot analysis and determination of protein concentration were used to observe the effects of drug-containing serum on osteoblast proliferation, gene expression of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in osteoblasts cultured in vitro. RESULTS AND CONCLUSION: After drug intervention, osteoblasts were spindle-shaped and trabs-shaped, which were concentrated and confluent with undefined boundary. Huangqi Sanxian decoction could increased the protein expression of osteoprotegerin in osteoblasts (P < 0.01), and also increased the protein expression of receptor activator of nuclear factor kappa B ligand (P < 0.01) to increase ratio of osteoprotegerin/receptor activator of nuclear factor kappa B ligand (P < 0.01). Huangqi Sanxian decoction can achieve the therapeutic effect on osteoporosis through regulating the expression of osteoprotegerin and receptor activator of nuclear factor kappa B ligand. Source


Shen S.L.,Liuzhou Hospital of Traditional Chinese Medicine
Zhonghua nan ke xue = National journal of andrology | Year: 2010

OBJECTIVE: To evaluate the clinical effect of the Chinese medicine Yangjing Decoction on idiopathic asthenospermia. METHODS: This study included 62 patients with idiopathic asthenospermia diagnosed with the computer-assisted semen analysis system and other methods based on the WHO guidelines. The patients were equally randomized to a trial and a control group, the former treated with Yangjing Decoction at the dose of 400 ml bid, and the latter with Yougui Capsules tid, both for a course of 6 months. Then we analyzed the changes in sperm concentration and percentage of grade a + b sperm in the patients, as well as the pregnancy in their wives. RESULTS: Compared with the controls, the patients of the trial group showed a significantly elevated percentage of grade a + b sperm after 6 months medication (P < 0.01), though no statistically significant differences were observed in sperm concentration (P > 0.05). The total rate of effectiveness was 87.09% and 7 pregnancies were achieved in the trial group, as compared with 66.74% and 3 pregnancies in the controls (P < 0.05). CONCLUSION: Yangjing Decoction can significantly improve sperm vitality, and has a desirable effect on idiopathic asthenospermia. Source


Lin J.,Guangxi Medical University | Zeng D.,Guangxi University of Science and Technology | He H.,Guangxi Medical University | Tan G.,Guangxi Medical University | And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

Objectives: To evaluate the therapeutic value of treatment model of conservative surgery in combination with chemotherapy for malignant ovarian germ cell tumor (MOGCT). Methods: A multi-center, prospective clinical trial was conducted on patients with MOGCT confirmed by intraoperative frozen section diagnosis during 2009 and 2013. These patients were randomly allocated into conservative surgery group (n=33) or extensive surgery group (n=36), and received either conservative or extensive surgery followed by chemotherapy. The health economics, safety, rate of regular menstruation and pregnancy, and efficacy of both models were compared. Results: (1) Health economics: The average initial hospital stays and hospital costs in conservative surgery group was significantly lower compared with extensive surgery group (P<0.01). (2) Safety: the average length of operation, amount of bleeding, recovery time of intestinal tract, and rate of operative complications in conservative surgery group was significantly lower compared with extensive surgery group (P<0.01). No significant difference in average time of indwelling urinary catheter was observed between the two groups (P>0.05). (3) The postoperative rate of both regular menstruation and pregnancy in conservative surgery group was similar to that in extensive surgery group (P>0.05). (4) Efficacy: no significant difference in the 3-year and 5-year accumulate survival rate or accumulate progressive-free survival rate was detected between the two groups (P>0.05). Conclusions: The treatment model of conservative surgery can reduce the cost of surgery and ensure the safety, and improve the quality of patients’ life without affecting the prognosis of MOGCT. © 2016, E-Century Publishing Corporation. All rights reserved. Source


Liu B.X.,Liuzhou Hospital of Traditional Chinese Medicine
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2011

To study the therapeutic efficacy of embedding thread according to staging and wholism syndrome differentiation and its effect on correlated indices of patients with postmenopausal osteoporosis. 135 patients with postmenopausal osteoporosis were randomly assigned to the control group A (treated with Calcichew D3 Tablet), the control group B (treated with Calcichew D3 Tablet and Xianling Gubao Capsule), and the treatment group (treated with Calcichew D5 Tablet and embedding thread according to staging and wholism syndrome differentiation). The visual analogue scale (VAS), Chinese medicine syndrome integral, and the quality of life scale before treatment, 3 months after treatment, and 6 months after treatment were assessed. Changes of the lumbar bone mineral density (BMD) and the serum level of estradiol (E2) were also assessed before and after six-month treatment. And the therapeutic efficacy of each group was also assessed after 6 months of treatment. Before treatment, there was no significant difference in scores of VAS, Chinese medicine syndrome integral and the quality of life scale, the.serum level of E2, and the lumbar BMD of the patients in three groups (all P>0.05). After three months of treatment, there was significant difference in scores of VAS, Chinese medicine syndrome integral and the quality of life scale of the patients in the three groups (all P<0.01). Of them, the improvement of the three indices in the control group A was the worst in three groups (P< 0.05, P<0.01). The VAS in the treatment group was superior to those in control group B (P<0.01). But the difference of Chinese medicine syndrome integral and the quality of life scale was insignificant in the three groups. After six months of treatment, significant difference was shown in the scores of VAS, Chinese medicine syndrome integral, or the quality of life scale of the patients in the three groups when compared with the corresponding index before treatment and after three months of treatment (all P<0.01). Of them the improvement of the three indices of patients in the treatment group and the control group B was better than that in the control group A (all P<0.01), and the improvement in the treatment group were superior to that in the control group B (P<0.05, P<0. 01). Significant difference was shown in the serum level of E2 and the lumbar BMD of the patients in the treatment group and the control group B when compared with before treatment of the same group (both P<0.01). But there was no difference in the control group A between before and after treatment, with better effects obtained in the treatment group and the control group B. And the serum level of E, of the patients in the treatment group after treatment was higher than that in the control group B (P<0.01), but there was no difference in the lumbar BMD. The therapeutic efficacy in the treatment group and the control group B were superior to that in the control group A (P<0.01, P<0.05), but no difference existed between the treatment group and the control group B. The therapy of embedding thread according to staging and wholism syndrome differentiation could reduce the scores of VAS and Chinese medicine syndrome integral, enhance the serum lever of E2, the quality of life scale and the lumbar BMD of patients with postmenopausal osteoporosis. So it was an effective method. Source

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