Lithuanian Veterinary Academy

Kaunas, Lithuania

Lithuanian Veterinary Academy

Kaunas, Lithuania
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Bartkiene E.,Lithuanian Veterinary Academy | Juodeikiene G.,Kaunas University of Technology | Basinskiene L.,Kaunas University of Technology | Liukkonen K.-H.,Finnish Food Safety Authority | And 2 more authors.
LWT - Food Science and Technology | Year: 2011

The aim of this work was to investigate the production of enterolactone (ENL) and enterodiol (END) both enterolignans, from their precursors by the action of intestinal microflora and their relationship with non-starch polysaccharides (NSP) in common plant foods such as berries and vegetables. For the investigation of the bioconversion of plant lignans the technique of in vitro fermentation was used and the quantitative analysis of their metabolites ENL and END was performed by HPLC with coulometric electrode array detection. The enterolignan production from various berries ranged from 7.8 to 382.8 nmol/g as well as from vegetables - from 10.5 till 91.2 nmol/g. By comparing different kind of berries, the cloudberry, raspberry, and strawberry were the best enterolignan producers. Considering vegetables, potatoes produced the highest quantity of total enterolignans. Garlic, zucchini and broccoli were the other good producers of enterolignans in this product group. The quantitative relationship between NSP components and their associated lignan metabolites were determined. The results showed that there is a correlation between the particularities of fermented food matrices and the production of enterolignans. For berries, an intermediate correlation was found between the total NSP and ENL values. For vegetables, higher correlations between NSP and END were found. © 2010 Elsevier Ltd.


Sirvaityte J.,Kaunas University of Technology | Siugzdaite J.,Lithuanian Veterinary Academy | Valeika V.,Kaunas University of Technology
Revista de Chimie | Year: 2011

The aim of the study was to investigate the possibility to use the commercial essential oils of Eucalyptus slobulus and Lavandulae officinalis as alternative presewatives for various tanned leather. The analyses have shown the differences between commercial essential oils from different producers. It was determined that these differences did not have influence on antibacterial activity of essential oils or their mixture with leather fatliqourins emulsion. As a main result of this study, it is concluded that selected commercial essential oils can be used as presewation agents in leather tanning industry The received results have shown that leather presewed with 2-(thiocyanomthylthio) benzothiazole (TCMTB) have weaker protection after 24 weeks comparing with the samples treated with essential oil of Lavandulae officinalis.


Juodeikiene G.,Kaunas University of Technology | Basinskiene L.,Kaunas University of Technology | Vidmantiene D.,Kaunas University of Technology | Makaravicius T.,Kaunas University of Technology | And 2 more authors.
Catalysis Today | Year: 2011

Proteinaceous inhibitors of xylanase naturally occur in cereals where they are involved in various roles in the plant defence metabolism. This study focused on the inhibitors of xylanase present in local rye cultivars, and their influence on the efficiency of the fermentation processes during bioethanol production from rye residues in comparison with common wheat. Different origin xylanases from Thermomyces lanuginosus and Trichoderma reesei were the objects of the investigations. Kinetic studies of these xylanases in the presence of proteins with inhibitory activity indicated that Th. lanuginosus was found more sensitive to proteinaceous xylanase inhibitors presented in rye than T. reesei. The highest yield of xylose and arabinose was achieved by adding T. reesei to cell wall substrates, while Th. lanuginosus converted to arabinoxylans only into xylooligosaccharides and monosaccharide were not released. The activity of xylanase in composition with α-amylase and glucoamylase was selected to achieve a higher ethanol yield in the distillate. It improved the quality of bioethanol by increasing the content of ethanol and decreasing the concentrations of propanol, isobutanol, isoamyl and amyl alcohols and the methanol concentration. No significant differences were found between the contents of ethanol from different type of bran. © 2011 Elsevier B.V. All rights reserved.


Kudirkiene E.,Lithuanian Veterinary Academy | Buneviciene J.,Lithuanian Veterinary Academy | Brondsted L.,Copenhagen University | Ingmer H.,Copenhagen University | And 2 more authors.
International Journal of Food Microbiology | Year: 2011

While cross-contamination from equipment and scalding water containing Campylobacter jejuni is considered the main route of broiler carcass contamination during slaughtering, alternative sources of C. jejuni may have been overlooked because only a limited number of studies focus on sampling of one broiler flock along the entire food chain and not many include the slaughterhouse environment. In the present study we have traced the changes of C. jejuni genotypes within one broiler flock from the beginning of rearing to the final product at the slaughterhouse with the aim to evaluate the dynamics and possible sources of carcass contamination with C. jejuni. Genotyping of 345 isolates of C. jejuni by flaA-RFLP revealed ten different flaA genotypes of C. jejuni along the broiler meat production chain. Broiler fillets were mainly contaminated with flaA genotypes found on the surfaces of slaughterhouse equipment and in the scalding water after cleaning and disinfection. Finally, it was clearly demonstrated that C. jejuni isolates remaining in the slaughterhouse environment after disinfection is a potential source of broiler meat contamination. Thus, identification of the mechanisms that allow such strains to persist in the slaughterhouse and survive cleaning is important for the establishment of future practices that will ensure sufficient reduction of C. jejuni in the slaughterhouse environment. © 2010 Elsevier B.V.


Kudirkiene E.,Lithuanian Veterinary Academy | Malakauskas M.,Lithuanian Veterinary Academy | Malakauskas A.,Lithuanian Veterinary Academy | Bojesen A.M.,Copenhagen University | Olsen J.E.,Copenhagen University
Journal of Applied Microbiology | Year: 2010

Aims: The aim of the study was to investigate the flock prevalence of Campylobacter jejuni and Campylobacter coli in broiler farms in Lithuania and to identify possible persistent strains of Camp. jejuni using amplified fragment length polymorphism (AFLP) typing method. Methods and Results: During 1 year, 42 broiler flocks from 9 broiler farms were examined to determine the prevalence of Campylobacter-positive broiler flocks in Lithuania. Among 42 broiler flocks examined, 31 flocks (73·8%) were positive for Camp. jejuni and 17 flocks (40·48%) for Camp. coli. Campylobacter jejuni isolates were genotyped by AFLP method using BspDI and BglII restriction enzymes. Typing of 190 isolates generated 50 AFLP genotypes with the highest diversity of strains found in the summer season. Each farm showed one or more predominant AFLP types, and one AFLP type (A32) was found in five broiler farms over a 1-year period. Conclusions: Campylobacter jejuni and Camp. coli are highly prevalent in broiler farms in Lithuania. Farm-specific genotypes were identified in all farms examined. Type A32 was present and persisted in different broiler farms, and a common source of transmission of Camp. jejuni was suspected. Significance and Impact of the Study: For the first time, Camp. jejuni in broiler flocks has been genetically characterized in Lithuania. Persistent strains of Camp. jejuni were detected over one period at the beginning of broiler meat production chain and, therefore, the identification of contamination source of such strains and the mechanism of their particular ability to persist are crucial to establish effective control measures against Camp. jejuni infection in broiler farms. © 2009 The Society for Applied Microbiology.


Baliukoniene V.,Lithuanian Veterinary Academy | Bakutis B.,Lithuanian Veterinary Academy | Januskeviciene G.,Lithuanian Veterinary Academy | Miseikiene R.,Milking Training Center
Journal of Animal and Feed Sciences | Year: 2011

The impact of two tillage systems (conventional and no-tillage) on cereal contamination with fungi and Fusarium mycotoxins was investigated. Samples of soil, cereal seeds, seedlings and freshly harvested grain were taken from plots of an agricultural cooperative in Lithuania. All samples were subjected to mycological analyses. Grain samples were also used for analysis of mycotoxins. In the soil under no-tillage, contamination with fungai spores was 92.9% higher compared to the soil under conventional tillage. In the soil of the no-tillage system we found 20 fungal genera, while in the soil under conventional tillage the number of genera was 15% lower. On cereal seedlings in the no-tillage system fungal spore content was 24.6% higher (P>0.05) and detection frequency of Fusarium fungi was 22.9% higher (P>0.05) than on seedlings in the conventional system. During harvest in feeding grain grown in the conventional tillage system, 2.2-8.0 log 10 CFU g -1 fungal spores were detected, compared to 2.5-12.0 log 10 CFU g -1 in grain in the no-tillage system. A significantly higher grain contamination with Fusarium spp. was detected in the no-tillage system. However, the deoxynivalenol, zearalenone and T-2 toxin content of grain was not considerably influenced by the different tillage systems, although it varied between species.


Ribikauskas V.,Lithuanian Veterinary Academy | Ribikauskiene D.,Lithuanian Veterinary Academy | Skurdeniene I.,Lithuanian Veterinary Academy
World Rabbit Science | Year: 2010

In the same room of an experimental rabbit house 2 housing systems were compared, namely three pens to keep rabbits on bedding and six wire cages for unbedded rabbit keeping. In total, 69 New Zealand White crossbred rabbits 8-12 wk old were used in the study. The rabbits were kept in groups with 0.6 m 2 floor area per animal in pens with thinly scattered straw. Wire cages had no bedding and there were 4 rabbits in each cage and 0.4 m2 floor area per rabbit. The study lasted for 8 wk. Rabbit behaviour was observed once 1 wk for 24 h. Behaviour was recorded using a scan sampling every hour. Results were analysed by calculating the percentage frequency of each behaviour. Behaviour analysis according to the keeping method indicated that rabbits kept on bedding spent on average less time resting and grooming (41.53% and 5.18% vs. 53.60% and 8.48%) and more time eating (29.86% vs. 18.03%, respectively) in comparison with those kept in wire cages. There was a statistically significant positive correlation between environmental temperature, illumination, ammonia and carbon dioxide gas concentrations and activity of rabbits. © WRSA UPV 2003.


Lipinski K.,University of Warmia and Mazury | Szramko E.,University of Warmia and Mazury | Jeroch H.,Lithuanian Veterinary Academy | Matusevicius P.,Lithuanian Veterinary Academy
Annals of Animal Science | Year: 2012

One of the well known biological functions of betaine is that of a methyl donor. Therefore, betaine may partly replace choline and methionine in the diet. Another widely documented role of betaine is to restore and maintain the osmotic balance. As an organic osmotic compound, betaine regulates the water balance, thus exerting a stabilizing influence on tissue metabolism, particularly within the digestive tract. As a donor of methyl groups necessary for various reactions in the body, betaine is indirectly involved in lipid metabolism. Due to its metabolic functions, betaine is also believed to play a significant role in energy metabolism in pigs. Of particular note are the results of experiments in which a positive effect of betaine supplementation was observed as the energy content of the diet was decreased.


Ruzauskas M.,Lithuanian Veterinary Academy | Suziedeliene E.,Vilnius University | Siugzdiniene R.,Lithuanian Veterinary Academy | Seputiene V.,Vilnius University | Povilonis J.,Vilnius University
Journal of Food Safety | Year: 2010

The objective of the study was to evaluate the antimicrobial susceptibility of enterococci spread in raw products of poultry origin intended for human consumption in Lithuania. Samples were obtained from retail markets all over the country. Fifty-eight samples (83%) from a total of 70 tested were positive for Enterococcus spp.Enterococcus faecium (36.2%), E. faecalis (29.3%) and E. hirae (17.2%) were the most prevalent species. Susceptibility testing was based on a microdilution test. Results were interpreted according to clinical breakpoints. The most frequent resistance was demonstrated to tetracycline (84.5%), tylosin (64.5%), erythromycin (63.8%) and ciprofloxacin (36.2%). No resistant strains were found to vancomycin, linezolid and tigecycline. Susceptibility to antimicrobials varied among different species of enterococci. E. faecalis demonstrated more frequent resistance to tylosin, E. hirae- to nitrofuranes. E. faecium was more frequently resistant to penicillin and tetracycline. Both E. faecium and E. hirae demonstrated more frequent resistance to fluoroquinolones than E. faecalis. Susceptibility to other antimicrobials statistically was unreliable between these species of enterococci. © 2010 Wiley Periodicals, Inc.


Miezeliene A.,KTU Food Institute | Alencikiene G.,KTU Food Institute | Gruzauskas R.,Lithuanian Veterinary Academy | Barstys T.,Lithuanian Veterinary Academy
Biotechnology, Agronomy and Society and Environment | Year: 2011

Currently there is a focus on the development of functional poultry products capable of enrichment by selenium, vitamin E, iodine and fatty acids of the third order. Since there is a lack of data about various selenium sources and its synergistic effect on sensory and other properties of vitamin E enriched poultry, the objective of this research was to examine the effect of addition of selenium in broilers diet on meat quality. The amount of Se in the diet was increased from 0.15 mgkg 1 feed (control group) to 0.5 mg. kg 1 feed. Addition of Se had no signifcant effect (p > 0.05) on cooking and thawing losses, as well as on the majority of sensory attributes, adhesiveness, cohesiveness of chicken breast samples. Aftertaste (p < 0.05), hardness (p < 0.001) and resilience (p < 0.001) of the texture of the samples increased along with the increased amount of Se in bird diet. Mean values of the sensory attributes of thigh muscles showed no signifcant differences among the samples in case of intensities of odor and taste attributes; however, rmness and chewiness of the tested samples increased by increasing the amount of Se in the feed (p > 0.05). In addition, Se did not have signifcant infuence on meat cooking or thawing losses. The results of this study showed that 0.15 mg and 0.5 mg of selenium in complex with 40 mg of vitamin E could be added to broiler diet without having negative effect on technological or sensory properties of the broiler meat and acceptability.

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