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Kaunas, Lithuania

Bartkiene E.,Lithuanian Veterinary Academy | Juodeikiene G.,Kaunas University of Technology | Basinskiene L.,Kaunas University of Technology | Liukkonen K.-H.,Finnish Food Safety Authority | And 2 more authors.
LWT - Food Science and Technology | Year: 2011

The aim of this work was to investigate the production of enterolactone (ENL) and enterodiol (END) both enterolignans, from their precursors by the action of intestinal microflora and their relationship with non-starch polysaccharides (NSP) in common plant foods such as berries and vegetables. For the investigation of the bioconversion of plant lignans the technique of in vitro fermentation was used and the quantitative analysis of their metabolites ENL and END was performed by HPLC with coulometric electrode array detection. The enterolignan production from various berries ranged from 7.8 to 382.8 nmol/g as well as from vegetables - from 10.5 till 91.2 nmol/g. By comparing different kind of berries, the cloudberry, raspberry, and strawberry were the best enterolignan producers. Considering vegetables, potatoes produced the highest quantity of total enterolignans. Garlic, zucchini and broccoli were the other good producers of enterolignans in this product group. The quantitative relationship between NSP components and their associated lignan metabolites were determined. The results showed that there is a correlation between the particularities of fermented food matrices and the production of enterolignans. For berries, an intermediate correlation was found between the total NSP and ENL values. For vegetables, higher correlations between NSP and END were found. © 2010 Elsevier Ltd.

Sirvaityte J.,Kaunas University of Technology | Siugzdaite J.,Lithuanian Veterinary Academy | Valeika V.,Kaunas University of Technology
Revista de Chimie | Year: 2011

The aim of the study was to investigate the possibility to use the commercial essential oils of Eucalyptus slobulus and Lavandulae officinalis as alternative presewatives for various tanned leather. The analyses have shown the differences between commercial essential oils from different producers. It was determined that these differences did not have influence on antibacterial activity of essential oils or their mixture with leather fatliqourins emulsion. As a main result of this study, it is concluded that selected commercial essential oils can be used as presewation agents in leather tanning industry The received results have shown that leather presewed with 2-(thiocyanomthylthio) benzothiazole (TCMTB) have weaker protection after 24 weeks comparing with the samples treated with essential oil of Lavandulae officinalis.

Juodeikiene G.,Kaunas University of Technology | Basinskiene L.,Kaunas University of Technology | Vidmantiene D.,Kaunas University of Technology | Makaravicius T.,Kaunas University of Technology | And 2 more authors.
Catalysis Today | Year: 2011

Proteinaceous inhibitors of xylanase naturally occur in cereals where they are involved in various roles in the plant defence metabolism. This study focused on the inhibitors of xylanase present in local rye cultivars, and their influence on the efficiency of the fermentation processes during bioethanol production from rye residues in comparison with common wheat. Different origin xylanases from Thermomyces lanuginosus and Trichoderma reesei were the objects of the investigations. Kinetic studies of these xylanases in the presence of proteins with inhibitory activity indicated that Th. lanuginosus was found more sensitive to proteinaceous xylanase inhibitors presented in rye than T. reesei. The highest yield of xylose and arabinose was achieved by adding T. reesei to cell wall substrates, while Th. lanuginosus converted to arabinoxylans only into xylooligosaccharides and monosaccharide were not released. The activity of xylanase in composition with α-amylase and glucoamylase was selected to achieve a higher ethanol yield in the distillate. It improved the quality of bioethanol by increasing the content of ethanol and decreasing the concentrations of propanol, isobutanol, isoamyl and amyl alcohols and the methanol concentration. No significant differences were found between the contents of ethanol from different type of bran. © 2011 Elsevier B.V. All rights reserved.

Ruzauskas M.,Lithuanian Veterinary Academy | Suziedeliene E.,Vilnius University | Siugzdiniene R.,Lithuanian Veterinary Academy | Seputiene V.,Vilnius University | Povilonis J.,Vilnius University
Journal of Food Safety | Year: 2010

The objective of the study was to evaluate the antimicrobial susceptibility of enterococci spread in raw products of poultry origin intended for human consumption in Lithuania. Samples were obtained from retail markets all over the country. Fifty-eight samples (83%) from a total of 70 tested were positive for Enterococcus spp.Enterococcus faecium (36.2%), E. faecalis (29.3%) and E. hirae (17.2%) were the most prevalent species. Susceptibility testing was based on a microdilution test. Results were interpreted according to clinical breakpoints. The most frequent resistance was demonstrated to tetracycline (84.5%), tylosin (64.5%), erythromycin (63.8%) and ciprofloxacin (36.2%). No resistant strains were found to vancomycin, linezolid and tigecycline. Susceptibility to antimicrobials varied among different species of enterococci. E. faecalis demonstrated more frequent resistance to tylosin, E. hirae- to nitrofuranes. E. faecium was more frequently resistant to penicillin and tetracycline. Both E. faecium and E. hirae demonstrated more frequent resistance to fluoroquinolones than E. faecalis. Susceptibility to other antimicrobials statistically was unreliable between these species of enterococci. © 2010 Wiley Periodicals, Inc.

Lipinski K.,University of Warmia and Mazury | Szramko E.,University of Warmia and Mazury | Jeroch H.,Lithuanian Veterinary Academy | Matusevicius P.,Lithuanian Veterinary Academy
Annals of Animal Science | Year: 2012

One of the well known biological functions of betaine is that of a methyl donor. Therefore, betaine may partly replace choline and methionine in the diet. Another widely documented role of betaine is to restore and maintain the osmotic balance. As an organic osmotic compound, betaine regulates the water balance, thus exerting a stabilizing influence on tissue metabolism, particularly within the digestive tract. As a donor of methyl groups necessary for various reactions in the body, betaine is indirectly involved in lipid metabolism. Due to its metabolic functions, betaine is also believed to play a significant role in energy metabolism in pigs. Of particular note are the results of experiments in which a positive effect of betaine supplementation was observed as the energy content of the diet was decreased.

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