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Fang R.,Forestry Academy of Zhejiang Province | Yang S.,Forestry Academy of Zhejiang Province | Yang S.,Lishui Academy of Forestry | Wang Y.,Forestry Academy of Zhejiang Province | Qian H.,Forestry Academy of Zhejiang Province

Paclitaxel (PTX) is usual for the treatment of a variety of malignancies, however, its applications are greatly limited due to its poor water solubility. Over the past years, there has been a considerable research interest in the area of nanoscale drug delivery systems (DDSs) as carrier for PTX due to their solubilization, safety, targeting and controlled release. There are many different types and shapes of nanoscale DDSs that have been prepared to deliver PTX, including nanoliposome, lipid nanoemulsion, nanosuspension, nanocapsule, nanofiber, nanotube, nanopolymersome, micelle and nanoparticle (NP). Nanoscale DDSs can be based on lipids, proteins, polysaccharides, polymers or other materials. The recent strategic developments of PTX formulation have been discussed with emphasis on lipid-, polymer- and protein-based nanoscale DDSs. Here we focus on the comparative analysis of the preparation, morphology, solubilization, targeting, penetrability, controllability and efficacy profile of various PTX-loaded nanoscale DDSs, which were reported in the different researches. Meanwhile the advantages and disadvantages are also discussed for each type of DDS. Furthermore, the current review embodies an in-depth discussion of human serum albumin (HSA) NP formulation, which showed significantly great efficacy and low toxicity. All the information obtained in this review might shed light on designing new and better nanoscale PTX formulations for potential anticancer applications in the clinic. © 2015 World Scientific Publishing Company. Source

Zhong Y.,Environment and Plant Protection Institute of Chinese Academy of Tropical Agriculture | Li Z.,Lishui Academy of Forestry | Li Z.,Central South University of forestry and Technology | Wei M.,Central South University of forestry and Technology

The Pachyprotasis melanosoma group is defined as a part of the former P. rapae group, and a key to this species of this group from China is provided. Six new species from China are described in this paper: P. coximaculata Zhong & Wei, sp. n.; P. coxipunctata Zhong & Wei, sp. n.; P. hengshani Zhong & Wei, sp. n.; P. maculoscutellata Zhong & Wei, sp. n.; P. pailongensis Zhong & Wei, sp. n. and P. qilianica Zhong & Wei, sp. n. Copyright © 2015 Magnolia Press. Source

Hong Z.,Lishui Academy of Forestry | Hong Z.,Lishui Vocational & Technical College | Zhu L.-J.,Zhejiang University | Fu X.-Q.,Zhejiang University | And 2 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal

In this paper, we studied in vitro culture of Bredia amoena by using leaf explants, including callus induction, adventitious shoot differentiation and plantlet regeneration. The results showed that the best medium for callus induction and adventitious shoot differentiation was MS+6-BA 1.0 mg·L-1+2,4-D 0.3 mg·L-1, with an induction rate of 100% and a differentiation rate of 85.83%. The best multiplication medium was MS+6-BA 1.0 mg·L-1+IBA 0.3 mg·L-1, the multiplication coefficient reached 7.38, and the shoots induced were strong. The optimum rooting medium was MS+NAA 0.5 mg·L-1+activated carbon (AC) 1 g·L-1, and the rooting rate reached 100%. The strong rooting plantlets were transplanted into greenhouse and the survival rate was 90%. ©, 2015, Science Press. All right reserved. Source

Li H.,Zhejiang Agriculture And forestry University | Li H.,Chinese Academy of Agricultural Sciences | Liu J.,Zhejiang Agriculture And forestry University | Zhu G.,Forestry Technology Popularization Master Station of Lishui | And 2 more authors.
Journal of the Chinese Cereals and Oils Association

A HPLC method for the determination of the two major flavonol glycosides {kaempferol-3-O-β-D-glucopyranosyl-[(2→1)-O-β-D-glucopyranosyl]-(6→1)-O-α-L-rhamnopynanoside (I) and kaempferol-3-O-[2-O-β-D-xylopyranosyl-6O-α-L-rhamnopyranosyl]-β-D-glucopyranoside (II)} from Camellia oleifera seeds was established, which was used to evaluate flavonoids contents among varieties of 'Chang Lin' series. Furthermore, the extraction process of the two major flavonol glycosides in Camellia oleifera seeds was optimized. The determination was performed on a Venusil MP C18 Column with a linear gradient elution consisting of acetonitrile and 0.1% phosphoric acid solution at a flow rate of 1.0 mL/min. The detection wavelength was 220 nm and column temperature was 25℃. An orthogonal test with methanol concentration, solid-liquid ratio, extraction temperature and time as factors was designed to analyze the extraction efficiency of flavonoid glycosides. The two flavonol glycosides could be extracted most on the condition of 80% methanol, solid-liquid ratio 1:50, and reflux extraction 60 min at 80℃. The contents of two major flavonol glycosides were ranged from 1.28% to 2.98% and from 1.42% to 2.37% respectively. This determination method has the advantages of more specificity, good accuracy and repeatability, which can be used to determinate the contents of main flavonol glycosides in different varieties of Camellia oleifera seeds. ©, 2014, Editorial Department, Chinese Cereals and Oils Association. All right reserved. Source

Huang J.,Zhejiang University | Shi K.,Forestry Bureau of Changxing County | Li Z.,Lishui Academy of Forestry | Wu H.,Forestry Bureau of Changxing County
Entomological News

The genus Pseudozygoneura Steffan (1969) is recorded and revised from China for the first time, and includes eleven species. Six new species, P. hexacantha sp. nov., P. brachyacantha sp. nov., P. bifida sp. nov., P. triacantha sp. nov., P. robustispina sp. nov. and P. quadridentata sp. nov. are described herein and illustrated in detail. Another five species, P. latidactyla Hippa, Vilkamaa & Heinakroon, 1998, P. index Hippa, Vilkamaa & Heinakroon, 1998, P. kirkspriggsi Hippa, Vilkamaa & Heinakroon, 1998, P. flagelloparva Hippa, Vilkamaa & Heinakroon, 1998 and P. lagena Hippa, Vilkamaa & Heinakroon, 1998 are reported. Geographical distribution of the eleven Chinese species is provided, as well as a key to all Chinese species. Source

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