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Liu Y.-G.,Yantai University | Liu L.-X.,Yantai University | Liu L.-X.,Linyi Academy of Agricultural science | Xing S.-C.,Qingdao University
Conservation Genetics Resources | Year: 2014

Thirteen polymorphic microsatellite loci have been isolated and characterized from Cynoglossus semilaevis for the purpose of protecting its wild resources. They had between three and nine alleles. Observed and expected heterozygosities varied from 0.57 to 0.93, and from 0.56 to 0.82, respectively. All the loci conformed to Hardy-Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected among the loci (P > 0.004). They have provided sufficient level of genetic diversity to investigate the fine-scale population structure and species conservation and management in the C. semilaevis. © 2014 Springer Science+Business Media Dordrecht. Source


Li C.-F.,Chinese Academy of Agricultural Sciences | Tao Z.-Q.,Chinese Academy of Agricultural Sciences | Liu P.,Shandong University | Zhang J.-W.,Shandong University | And 3 more authors.
Journal of Integrative Agriculture | Year: 2015

The grain yield of maize has increased continuously in past decades, largely through hybrid innovation, cultivation technology, and in particular, recent genetic improvements in photosynthesis. Elite inbred lines are crucial for innovating new germplasm. Here, we analyzed variations in grain yield and a series of eco-physiological photosynthetic traits after anthesis in sixteen parental lines of maize (Zea mays L.) released during three different eras (1960s, 1980s, 2000s). We found that grain yield and biomass significantly increased in the 2000s than those in the 1980s and 1960s. Leaf area, chlorophyll, and soluble protein content slowly decreased, and maintained a higher net photosynthesis rate (Pn) and improved stomatal conductance (Gs) after anthesis in the 2000s. In addition, the parental lines in the 2000s obtained higher actual photochemistry efficiency (ΦPSII) and the maximum PSII photochemistry efficiency (Fv/Fm), which largely improved light partitioning and chlorophyll fluorescence characteristic, including higher photochemical and photosystem II (PSII) reaction center activity, lower thermal energy dissipation in antenna proteins. Meanwhile, more lamellae per granum within chloroplasts were observed in the parental lines of the 2000s, with a clear and complete chloroplast membrane, which will greatly help to improve photosynthetic capacity and energy efficiency of ear leaf in maize parental lines. It is concluded that grain yield increase in modern maize parental lines is mainly attributed to the improved chloroplast structure and more light energy catched for the photochemical reaction, thus having a better stay-green characteristic and stronger photosynthetic capacity after anthesis. Our direct physiological evaluation of these inbred lines provides important information for the further development of promising maize cultivars. © 2015 Chinese Academy of Agricultural Sciences. Source


Wang C.,Anhui University of Science and Technology | Liu L.-X.,Linyi Academy of Agricultural science | Liu Y.-G.,Xinjiang University
Conservation Genetics Resources | Year: 2015

Twenty polymorphic mirosatellite markers have been developed and characterized from RAPD product in Populus euphratica for protection of its natural resources. Polymorphisms of these microsatellite markers were evaluated in a natural population of 30 individuals collected from Xinjiang Region in China. The number of alleles ranged from 3 to 12. Observed and expected heterozygosities varied from 0.53 to 0.97, and from 0.56 to 0.94, respectively. All the microsatellite loci conformed to Hardy–Weinberg equilibrium after Bonferroni correction. They have showed sufficient level of polymorphisms to estimate the population structure, genetic diversity and species conservation. It is an efficient method to isolate microsatellite markers from RAPD product in P. euphratica. © 2015, Springer Science+Business Media Dordrecht. Source


Liu Y.-G.,Xinjiang University | Liu L.-X.,Linyi Academy of Agricultural science
Conservation Genetics Resources | Year: 2015

A total of 22 polymorphic simple sequence repeat (SSR) markers have been developed and characterized from AFLP sequences of Crassostrea gigas in order to protect its natural resources. Polymorphisms of these SSR markers were evaluated in a natural population of 30 individuals collected from Shandong peninsula in China. The number of alleles ranged from three to thirteen. Observed and expected heterozygosities varied from 0.50 to 0.97, and from 0.55 to 0.91, respectively. All the SSR loci conformed to Hardy–Weinberg equilibrium after Bonferroni correction. They have showed sufficient level of polymorphisms to estimate the genetic diversity, population structure and species conservation in the C. gigas. © 2015, Springer Science+Business Media Dordrecht. Source


Zhou L.,Huazhong Agricultural University | Zhou L.,Jiangsu Academy of Agricultural Sciences | Zhou L.,Nanjing Agricultural University | Luo L.,BGI Shenzhen | And 14 more authors.
Plant Genome | Year: 2016

Soybean, an important source of vegetable oils and proteins for humans, has undergone significant phenotypic changes during domestication and improvement. However, there is limited knowledge about genes related to these domesticated and improved traits, such as flowering time, seed development, alkaline-salt tolerance, and seed oil content (SOC). In this study, more than 106,000 single nucleotide polymorphisms (SNPs) were identified by restriction site associated DNA sequencing of 14 wild, 153 landrace, and 119 bred soybean accessions, and 198 candidate domestication regions (CDRs) were identified via multiple genetic diversity analyses. Of the 1489 candidate domestication genes (CDGs) within these CDRs, a total of 330 CDGs were related to the above four traits in the domestication, gene ontology (GO) enrichment, gene expression, and pathway analyses. Eighteen, 60, 66, and 10 of the 330 CDGs were significantly associated with the above four traits, respectively. Of 134 traitassociated CDGs, 29 overlapped with previous CDGs, 11 were consistent with candidate genes in previous trait association studies, and 66 were covered by the domesticated and improved quantitative trait loci or their adjacent regions, having six common CDGs, such as one functionally characterized gene Glyma15 g17480 (GmZTL3). Of the 68 seed size (SS) and SOC CDGs, 37 were further confirmed by gene expression analysis. In addition, eight genes were found to be related to artificial selection during modern breeding. Therefore, this study provides an integrated method for efficiently identifying CDGs and valuable information for domestication and genetic research. © Crop Science Society of America. Source

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