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Kalmar, Sweden

Linnaeus University is a state university in the Småland region of Sweden. It has two campuses, one in Växjö and one in Kalmar. Linnaeus University was established in 2010 by a merger of former Växjö University and Kalmar University , and has been named in honour of botanist Carl Linnaeus. Wikipedia.

Mansson A.,Linnaeus University
Journal of Muscle Research and Cell Motility

This review describes the development towards actomyosin based nanodevices taking a starting point in pioneering studies in the 1990s based on conventional in vitro motility assays. References are given to parallel developments using the kinesin-microtubule motor system. The early developments focused on achieving cargotransportation using actin filaments as cargo-loaded shuttles propelled by surface-adsorbed heavy meromyosin along micro- and nanofabricated channels. These efforts prompted extensive studies of surface-motor interactions contributing with new insights of general relevance in surface and colloid chemistry. As a result of these early efforts, a range of complex devices have now emerged, spanning applications in medical diagnostics, biocomputation and formation of complex nanostructures by selforganization. In addition to giving a comprehensive account of the developments towards real-world applications an important goal of the present review is to demonstrate important connections between the applied studies and fundamental biophysical studies of actomyosin and muscle function. Thus the manipulation of the motor proteins towards applications has resulted in new insights into methodological aspects of the in vitro motiliy assay. Other developments have advanced the understanding of the dynamic materials properties of actin filaments. © Springer Science+Business Media B.V. 2012. Source

Proteins interact with ions in various ways. The surface of proteins has an innate capability to bind ions, and it is also influenced by the screening of the electrostatic potential owing to the presence of salts in the bulk solution. Alkali metal ions and chlorides interact with the protein surface, but such interactions are relatively weak and often transient. In this paper, computer simulations and analysis of protein structures are used to characterize the interactions between ions and the protein surface. The results show that the ion-binding properties of protein residues are highly variable. For example, alkali metal ions are more often associated with aspartate residues than with glutamates, whereas chlorides are most likely to be located near arginines. When comparing NaCl and KCl solutions, it was found that certain surface residues attract the anion more strongly in NaCl. This study demonstrates that protein-salt interactions should be accounted for in the planning and execution of experiments and simulations involving proteins, particularly if subtle structural details are sought after. © 2011 American Chemical Society. Source

Gossling S.,Linnaeus University
Tourism Management

Tourism is increasingly recognized as a significant water-consuming sector on local, regional and global scales. As a consequence, the efficient use of water resources is now considered a key sustainability challenge for the tourism industry. To date, most research has focused on direct (on site) water consumption, with tourism water management based almost exclusively on direct water use benchmarks. This paper argues that such an approach overlooks the complexity of 'local' and 'global' water use, with local water use affecting sustainable water use in the destination and global water use representing the sustainability of water embodied in goods produced elsewhere, including fuels and food. Focussing on tourism accommodation as the locus of tourism water consumption, conventional water indicators are reviewed and discussed, and knowledge gaps identified. New data accounting for food consumption are then presented for a case study of resort hotels in Rhodes, Greece. The results are used to develop a novel set of performance indicators suitable for resort hotels and other accommodation, considering water availability, planning and operation, as well as complexities of direct vis-à-vis indirect water consumption. The findings suggest a significant potential for water and related cost savings, indicating that holistic water management should be an operational imperative. © 2014 Elsevier Ltd. Source

Despite intense efforts to elucidate the molecular mechanisms that determine the maximum shortening velocity and the shape of the force-velocity relationship in striated muscle, our understanding of these mechanisms remains incomplete. Here, this issue is addressed by means of a four-state cross-bridge model with significant explanatory power for both shortening and lengthening contractions. Exploration of the parameter space of the model suggests that an actomyosin-ADP state (AM*ADP) that is separated from the actual ADP release step by a strain-dependent isomerization is Important for determining both the maximum shortening velocity and the shape of the force-velocity relationship. The model requires a velocity-dependent, cross-bridge attachment rate to account for certain experimental findings. Of interest, the velocity dependence for shortening contraction is similar to that for population of the AM*ADP state (with a velocity-independent attachment rate). This accords with the idea that attached myosin heads in the AM*ADP state position the partner heads for rapid attachment to the next site along actin, corresponding to the apparent increase in attachment rate in the model. © 2010 by the Biophysical Society. Source

Friedman R.,Linnaeus University
Biochemical Journal

Amyloid-related diseases are a group of illnesses in which an abnormal accumulation of proteins into fibrillar structures is evident. Results from a wide range of studies, ranging from identification of amyloid-β dimers in the brain to biophysical characterization of the interactions between amyloidogenic peptides and lipid membranes during fibril growth shed light on the initial events which take place during amyloid aggregation. Accounts of fibril disaggregation and formation of globular aggregates due to interactions with lipids or fatty acids further demonstrate the complexity of the aggregation process and the difficulty to treat amyloid-related diseases. There is an inherent difficulty in generalizing from studies of aggregation in vitro, but the involvement of too many cellular components limits the ability to follow amyloid aggregation in a cellular (or extracellular) context. Fortunately, the development of experimental methods to generate stable globular aggregates suggests new means of studying the molecular events associated with amyloid aggregation. Furthermore, simulation studies enable deeper understanding of the experimental results and provide useful predictions that can be tested in the laboratory. Computer simulations can nowadays provide molecular or even atomistic details that are experimentally not available or very difficult to obtain. In the present review, recent developments on modelling and experiments of amyloid aggregation are reviewed, and an integrative account on how isolated interactions (as observed in vitro and in silico) combine during the course of amyloidrelated diseases is presented. Finally, it is argued that an integrative approach is necessary to get a better understanding of the protein aggregation process. ©The Authors Journal compilation © 2011 Biochemical Society. Source

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