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Lincang, China

Jin L.,Sun Yat Sen University | Jin L.,Lincang Teachers College | Wang T.,Nanjing Southeast University | Wang T.,Jinan University | And 6 more authors.
Journal of Materials Chemistry B | Year: 2013

The development of modern biomedical nanotechnology requires conductive polymeric nanofibers with excellent mechanical and biocompatible properties to meet the needs of practical applications in complex biological systems. In the study, we developed a novel facile method to fabricate poly(3,4- ethylenedioxythiophene) (PEDOT) nanofiber mats by electrospinning combined with in situ interfacial polymerization. The PEDOT nanofiber mats displayed superior mechanical properties (tensile strength: 8.7 ± 0.4 MPa; Young's modulus: 28.4 ± 3.3 MPa) and flexibility, which can almost be restored to its original shape even after serious twisting and crimping. Especially, from the results of the cellular morphology and proliferation of human cancer stem cells (hCSCs) cultured on the PEDOT nanofiber mats for 3 days, evidence was provided that the PEDOT nanofiber mats have similar biocompatibility to tissue culture plates (TCPs). Combined with an outstanding electrical conductivity of 7.8 ± 0.4 S cm-1, these excellent mechanical and biocompatible properties make the PEDOT nanofiber mats promising candidates in biotechnology applications, such as electroactive substrates/scaffolds for tissue engineering, drug delivery, cell culture, and implanted electrodes. © The Royal Society of Chemistry 2013. Source


Jin L.,Sun Yat Sen University | Jin L.,Lincang Teachers College | Feng Z.-Q.,Sun Yat Sen University | Zhu M.-L.,Sun Yat Sen University | And 3 more authors.
Journal of Biomedical Nanotechnology | Year: 2012

In this study, a novel three-dimensional fluffy PPy conductive fibrous scaffold (3D-cFSs) was fabricated by electrospinning technique combined with situ surface polymerization. Chemical compositions, morphology were characterized by fourier transform infrared (FTIR) and scanning electron microscopy (SEM). The results showed that the average diameter of PPy coated PLLA fibers in the 3D-cFSs was 2.086 μm, the thickness of PPy nano-layer was ∼45 nm. These PPy coated PLLA fibers were in discrete state, the size of interconnected pores in the 3D-cFSs was from 50 μm to 100 μm, this unique structure ensured that cells can entry into internal of 3D-cFSs smoothly without any other extra help to achieve three-dimensional cell culture (3D-culture). Rat pheochromocytoma 12 (PC12) cells (as model cell) were cultured in the 3D-cFSs to evaluate its potential application for nerve tissue engineering. The interaction between cell and scaffold was test by detecting the cell proliferation, viability, and morphology. After 3 days culture, the number of PC12 in 3D-cFSs were much higher than that on the conductive fibrous meshes (cFMs) and well developed cell-fibers constructs were observed from fluorescence image and SEM of PC12 in the central of 3D-cFSs. These results showed that the 3D-cFSs provided cell 3D-culture, and improved cell growth. Therefore, we suggest that the 3D-cFSs maybe a suitable scaffold for the nerve tissue engineering as cells substrate to apply electrical stimulation. Copyright © 2012 American Scientific Publishers All rights reserved. Source


Jin L.,Sun Yat Sen University | Jin L.,Lincang Teachers College | Wang T.,Nanjing Southeast University | Wang T.,Jinan University | And 5 more authors.
Journal of Materials Chemistry | Year: 2012

Three dimensional (3D) cell culture in functional scaffolds to mimic the cell natural growth state is important for the construction of cell based implants in vitro for tissue engineering applications. Herein, we report a novel fluffy polypyrrole (PPy) fibrous scaffold (fluffy-PPy scaffold) fabricated by means of an improved electrospinning process combined with in situ surface polymerization, in which PPy hollow fibers are discrete from one another with deep interconnected pores of ∼100 μm. This unique spatial structure permits the easy entry of cells into the fluffy-PPy scaffold with no extra help to achieve complicated 3D cell culture methodologies. The cell proliferation and morphology of cardiomyocytes (as a model cell) cultured in the fluffy-PPy scaffold were tested over a 3 day culture period. Evidence was provided that cardiomyocytes entered into the interior of the fluffy-PPy scaffold and formed stable cell-fiber constructs, and the rate of cell proliferation was higher than that on a traditional electrospun PPy fibrous mesh (mesh-PPy scaffold) and tissue culture plates (TCP). These results demonstrate that the fluffy-PPy scaffold not only achieved 3D cell culture, but also resulted in increased cell proliferation. Therefore, we suggest that the fluffy-PPy scaffold may be an appropriate choice as a functional scaffold capable of supporting 3D cell culture in the field of cardiac tissue engineering. © 2012 The Royal Society of Chemistry. Source


Fu Y.-P.,Lincang Teachers College | Fu Y.-P.,Yunnan University | Li Y.-D.,Yunnan University
Chinese Physics Letters | Year: 2012

We calculate the production of real photons originating from the photoproduction in relativistic pp collisions. The Weizsäcker - Williams approximation in the photoproduction is considered. Numerical results agree with the experimental data from the Relativistic Heavy Ion Collider and the Large Hadron Collider. We find that the modification of the photoproduction is more prominent in large transverse momentum regions. © 2012 Chinese Physical Society and IOP Publishing Ltd. Source


Jin L.,Sun Yat Sen University | Jin L.,Lincang Teachers College | Wang T.,Nanjing Southeast University | Zhu M.-L.,Sun Yat Sen University | And 5 more authors.
Journal of Biomedical Nanotechnology | Year: 2012

Electrospinning is an exciting technique attracting more and more attention as a potential solution to the current challenges in the field of tissue engineering. This technique can be used to produce fibrous scaffolds with excellent biocompatibility and biodegradability, as well as suitable micro-/nano-structure to induce desired cellular activities and to guide tissue regeneration. In order to develop electrospun fibrous scaffolds for these applications, different biocompatible materials including natural polymers, synthetic polymers and inorganic substances and preparations have been used to fabricate electrospun fibers with different structures and morphologies. This review briefly describes the development of the technique, focusing on several typically electrospun materials, surface modification of electrospun fibers, and current applications in tissue engineering. Copyright © 2012 American Scientific Publishers All rights reserved. Source

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