Verneuil-sur-Seine, France
Verneuil-sur-Seine, France

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Chapuis R.,French National Institute for Agricultural Research | Delluc C.,Limagrain Europe | Debeuf R.,Limagrain Europe | Tardieu F.,French National Institute for Agricultural Research | Welcker C.,French National Institute for Agricultural Research
European Journal of Agronomy | Year: 2012

Resilience to soil water deficit is a priority of many maize breeding programmes. Its genetic analysis requires estimators that characterise each genotype in a reproducible way. We have tested and compared three methods for that, namely (i) the ability of hybrids to maintain leaf growth in a range of soil water potentials in a phenotyping platform, (ii) a direct estimator of resilience of seed number to water deficit in a network of field experiments and (iii) classical methods involving tolerance indices and variance analysis. A drought index was obtained by averaging soil water potential, measured with tensiometers, for the phenological phase during which seed number is determined (evaluated for each individual hybrid in each site). It closely correlated with seed number in each of the 19 hybrids that were analysed over 14 environmental situations in France, Hungary and Chile. The slope of the regression line between drought index and seed number, established for each hybrid, was taken as an estimate of the resilience to soil water deficit of that hybrid. Resilience estimated in this way varied 2-fold in the set of studied hybrids, and correlated with resilience of leaf growth to soil water deficit in short-term experiments in the phenotyping platform. In contrast, the resilience estimated via the interaction genotype × watering treatment resulted non-significant because of large differences in drought indices between genotypes in a given watering treatment. We therefore propose that direct estimation of resilience to water deficit is feasible in the field with a minimum amount of environmental measurements. This provides estimations that are consistent with those measured in a phenotyping platform. The combination of both estimates provides insight into the mechanisms associated with resilience of each hybrid, potentially contributing to accelerate the genetic gain. © 2011 Elsevier B.V.


Van Inghelandt D.,University of Hohenheim | Van Inghelandt D.,Limagrain GmbH | Melchinger A.E.,University of Hohenheim | Martinant J.-P.,Limagrain Europe | And 2 more authors.
BMC Plant Biology | Year: 2012

Background: Setosphaeria turcica is a fungal pathogen that causes northern corn leaf blight (NCLB) which is a serious foliar disease in maize. In order to unravel the genetic architecture of the resistance against this disease, a vast association mapping panel comprising 1487 European maize inbred lines was used to (i) identify chromosomal regions affecting flowering time (FT) and northern corn leaf blight (NCLB) resistance, (ii) examine the epistatic interactions of the identified chromosomal regions with the genetic background on an individual molecular marker basis, and (iii) dissect the correlation between NCLB resistance and FT.Results: The single marker analyses performed for 8 244 single nucleotide polymorphism (SNP) markers revealed seven, four, and four SNP markers significantly (α=0.05, amplicon wise Bonferroni correction) associated with FT, NCLB, and NCLB resistance corrected for FT, respectively. These markers explained individually between 0.36 and 14.29% of the genetic variance of the corresponding trait.Conclusions: The very well interpretable pattern of SNP associations observed for FT suggested that data from applied plant breeding programs can be used to dissect polygenic traits. This in turn indicates that the associations identified for NCLB resistance might be successfully used in marker-assisted selection programs. Furthermore, the associated genes are also of interest for further research concerning the mechanism of resistance to NCLB and plant diseases in general, because some of the associated genes have not been mentioned in this context so far. © 2012 Van Inghelandt et al.; licensee BioMed Central Ltd.


Patent
Limagrain Europe and French National Institute for Agricultural Research | Date: 2016-11-09

The present invention concerns an isolated polynucleotide responsible of haploid induction in maize plants and related processes. Additionally, the invention relates to plants that have been genetically transformed with the polynucleotide of the invention. The invention also relates to a process for screening a mutant plant population for enhanced haploid induction by using said isolated polynucleotide. The invention further relates to molecular markers associated with haploid induction in maize plants and their use in quality control for inducer lines.


Grant
Agency: European Commission | Branch: FP7 | Program: CP-FP | Phase: KBBE.2012.1.1-01 | Award Amount: 3.83M | Year: 2013

Seed quality is of paramount importance to agriculture, food security and the conservation of wild species. Considerable economic losses result from sub-optimal seed performance, undermining food security and livelihoods. Seed quality is strongly influenced by the environmental stresses experienced by the mother plant. Climate change will further exacerbate economic losses and decrease the predictability of seed yield and quality for the farmer. The looming challenges of climate change and food security require new knowledge of how stress impacts on seed quality, as well as a re-appraisal of optimal storage conditions. EcoSeed addresses these challenges by bringing together a group of distinguished European experts in seed science and converging sciences to characterise seed quality and resilience to perturbation. EcoSeed combines state-of the-art omics, epigenetics, and post-omics approaches, such as nuclear and chromatin compaction, DNA repair, oxidative and post-translational modifications to macromolecules, to define regulatory switchboards that underpin the seed phenotype. Special emphasis is placed on the stress signalling hub that determines seed fate from development, through storage, germination and seedling development, with a particular focus on seed after-ripening, vigour, viability and storability. Translation of new knowledge gained in model to crop and wild species is an integral feature of EcoSeed project design, which will create a step-change in our understanding of the regulatory switchboards that determine seed fate. Novel markers for seed quality and new omics information generated in this project will assist plant breeders, advise the seed trade and conservationists alike. In this way, EcoSeed will not only be proactive in finding solutions to problems of ensuring seed quality and storability but also play a leading role in enabling associated industries to better capture current and emerging markets.


Patent
Limagrain Europe | Date: 2010-11-05

The present invention relates to isolated nucleotide sequences useful for the production of plants with a modified embryo sac, embryo and or endosperm development, and to transgenic cells and plants transformed with the nucleotide sequences.


Patent
Limagrain Europe | Date: 2015-07-15

The invention relates to a method for genotyping individuals, through multiplexing genotyping, when samples carrying different loci of various individuals are pooled and genotyping is performed on this pool.


Patent
Limagrain Europe | Date: 2015-01-09

The invention relates to a method for genotyping individuals, through multiplexing genotyping, when samples carrying different loci of various individuals are pooled and genotyping is performed on this pool.


Patent
Limagrain Europe | Date: 2015-09-16

Disclosed is a method of producing hybrid seeds, in particular hybrid cereal seeds, comprising crossing a stand of shorter female (male sterile) plants (shorter than the fertile plants) with a stand of taller male fertile plants (taller than the sterile plants). The method comprises limiting the proportion of self-fertilized male seed in the final produced seed stock. In particular, the method limits the seed development on male plants after pollination to reduce or eliminate the proportion of self-fertilized male seed in the final produced seed stock. The method limits this proportion of self-fertilized male seed in the final produced seed stock, under a threshold value, preferably under a value to be compliant with a regulatory hybridity level. Limiting the seed stock of male plant comprises passing, at least once, a tool extending above the height of the shorter female plants, but below the level of the height of the taller male fertile plants, between anthesis, preferably end of anthesis, and harvest. The tool is intended to prevent or reduce normal development of these male fertile plants standing above this height. The crossing of female and male fertile plants comprises sowing the seeds as a mix or drilling male sterile seeds and male fertile plant seeds in separate lines. Preferably, said eliminating tool has means to apply an herbicide, preferably systemic, such as glyphosate.


Patent
Limagrain Europe | Date: 2011-04-13

The present invention relates to isolated nucleotide sequences useful for the production of plants with a modified embryo sac, embryo and/or endosperm development, e.g. female sterility, pathenogenetic embryo and/or autonomous endosperm development.


Patent
Limagrain Europe | Date: 2016-04-06

Method of continuously harvesting, chopping and parameter measuring for silage plants, comprising two successive plots designated A and B, plot A being harvested before plot B, comprising (a) harvesting and chopping plants from a plot, collecting all or part of the crop of the plot in a waiting bin, (b) transferring this crop from the waiting bin to a measuring bin, (c) determining silage parameter during the stay in the measuring bin, and (d) withdrawing crop from measuring bin, wherein step (a) for plot B occurs during step (c) for plot A, and step (b) for plot B is performed after steps (c) and (d) for plot A. Silage harvester comprising a silage collecting unit having a waiting bin (2) and a measuring bin (7), waiting bins exit being placed in communication with measuring bins entry, bins exit having monitored means allowing to close or open said exit, parameter measuring sensor allowing parameter determination in the measuring bin, electronic processing means recovering parameters and controlling opening/closing of waiting and measuring bins.

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