Vilnius, Lithuania
Vilnius, Lithuania

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PubMed | 4 Joint Stock Company Akiu Gydytoju Praktika, Vilnius University and 2 Light Conversion Ltd.
Type: Journal Article | Journal: Photomedicine and laser surgery | Year: 2015

The purpose of this study was to investigate the possible genotoxic impact of new generation 205nm femtosecond solid-state laser irradiation on the DNA of murine bone marrow cells in vitro, and to compare the DNA damage caused by both femtosecond and nanosecond UV laser pulses.Recent experiments of corneal stromal ablation in vitro and in vivo applying femtosecond UV pulses showed results comparable with or superior to those obtained using nanosecond UV lasers. However, the possible genotoxic effect of ultrashort laser pulses was not investigated.Mouse bone marrow cells were exposed to different doses of 205nm femtosecond, 213 and 266nm nanosecond lasers, and 254nm UV lamp irradiation. The comet assay was used for the evaluation of DNA damage.All types of irradiation demonstrated intensity-dependent genotoxic impact. The DNA damage induced depended mainly upon wavelength rather than on other parameters such as pulse duration, repetition rate, or beam delivery to a target.Both 205nm femtosecond and clinically applied 213nm nanosecond lasers pulses induced a comparable amount of DNA breakage in cells exposed to the same irradiation dose. To further evaluate the suitability of femtosecond UV laser sources for microsurgery, a separate investigation of the genotoxic and mutagenic effects on corneal cells in vitro and, particularly, in vivo is needed.

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