Iruma, Japan
Iruma, Japan

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Hayakawa Y.,Nagahama Institute of Bio-Science and Technology | Matsuno M.,Nagahama Institute of Bio-Science and Technology | Tanaka M.,Nagahama Institute of Bio-Science and Technology | Wada A.,Nagasaki University | And 5 more authors.
Journal of Peptide Science | Year: 2015

Artificial peptides designed for molecular recognition of a bacterial toxin have been developed. Vacuolating cytotoxin A protein (VacA) is a major virulence factor of Helicobacter pylori, a gram-negative microaerophilic bacterium inhabiting the upper gastrointestinal tract, particularly the stomach. This study attempted to identify specific peptide sequences with high affinity for VacA using systematic directed evolution in vitro, a cDNA display method. A surface plasmon resonance-based biosensor and fluorescence correlation spectroscopy to examine binding of peptides with VacA identified a peptide (GRVNQRL) with high affinity. Cyclization of the peptide by attaching cysteine residues to both termini improved its binding affinity to VacA, with a dissociation constant (Kd) of 58nm. This study describes a new strategy for the development of artificial functional peptides, which are promising materials in biochemical analyses and medical applications. Copyright © 2015 European Peptide Society and John Wiley & Sons, Ltd.


Hirama T.,Saitama University | Hirama T.,Hospital for Sick Children | Mogi H.,Lifetech Co. | Egashira H.,Saitama University | And 4 more authors.
Clinica Chimica Acta | Year: 2015

Currently molecular techniques are a broadly accepted tool for diagnosis and are able to benefit patients in clinical practice. The polymerase chain reaction (PCR) has been especially incorporated into practical applications that are already in widespread use across the globe. With regard to the initial DNA extraction from clinically relevant samples, a number of commercially available kits are commonly used and are also designed to be easy to handle and less labor-intensive. In this study, the pressure system extracting DNA in column-based kit was developed, and its utility was compared with the centrifuge method using sputum from patients who were diagnosed with pneumonia. Also, due to the compact size and rapid processing time, the practical application of the pressure-based system incorporated into an automated pipetting machine was evaluated through clinical study. Our data suggests that DNA extraction by pressure was capable of serving as a substitute for the centrifuge method, and the compact and automatic nature of the pressure system device provided rapid and valuable information for clinical practice. © 2015 Elsevier B.V.


Sun G.,Tokyo Metroplitan University | Abe N.,Japan Self Defense Forces Central Hospital | Sugiyama Y.,Tokyo Metropolitan Government | Nguyen Q.V.,Tokyo Metroplitan University | And 6 more authors.
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS | Year: 2013

After the outbreak of severe acute respiratory syndrome (SARS) in 2003, many international airport quarantine stations conducted fever-based screening to identify infected passengers using infrared thermography for preventing global pandemics. Due to environmental factors affecting measurement of facial skin temperature with thermography, some previous studies revealed the limits of authenticity in detecting infectious symptoms. In order to implement more strict entry screening in the epidemic seasons of emerging infectious diseases, we developed an infection screening system for airport quarantines using multi-parameter vital signs. This system can automatically detect infected individuals within several tens of seconds by a neural-network-based discriminant function using measured vital signs, i.e., heart rate obtained by a reflective photo sensor, respiration rate determined by a 10-GHz non-contact respiration radar, and the ear temperature monitored by a thermography. In this paper, to reduce the environmental effects on thermography measurement, we adopted the ear temperature as a new screening indicator instead of facial skin. We tested the system on 13 influenza patients and 33 normal subjects. The sensitivity of the infection screening system in detecting influenza were 92.3%, which was higher than the sensitivity reported in our previous paper (88.0%) with average facial skin temperature. © 2013 IEEE.


Sun G.,Tokyo Metroplitan University | Abe S.,Takasaka Clinic | Takei O.,Lifetech Co. | Hakozaki Y.,Japan Self Defense Forces Central Hospital | Matsui T.,Tokyo Metroplitan University
Communications in Computer and Information Science | Year: 2011

This paper aims to evaluate the efficacy of our non-contact infection screening system which uses Kohonen's self-organizing map (SOM) with Kmeans clustering algorithm. In this study, the linear discriminant analysis (LDA) used in our previous system was replaced by SOM with K-means clustering algorithm to increase accuracy. The system simultaneously measures heart rate, respiratory rate, and facial skin temperature. The evaluation was done using the same data which we used in our previous study. The data was based on the test on 57 influenza patients and 35 normal control subjects at Japan Self-defense Forces Central Hospital. The system showed higher sensitivity of 98% and negative predictive value (NPV) of 96% compared to our previous system (sensitivity of 89%, NPV of 83%). The system can be used as a public health measure at points of entry where high sensitivity is most required in order to prevent the spread of the pandemic. © 2011 Springer-Verlag.


Sun G.,Tokyo Metroplitan University | Vinh N.Q.,Tokyo Metroplitan University | Matsuoka A.,Tokyo Metroplitan University | Miyata K.,Tokyo Metroplitan University | And 7 more authors.
2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014 | Year: 2014

The outbreak of infectious diseases such as influenza, dengue fever, and severe acute respiratory syndrome (SARS) are threatening the global health. Especially, developing countries in the South-East Asia region have been at serious risk. Rapid and highly reliable screening of infection is urgently needed during the epidemic season at mass gathering places, such as airport quarantine facilities, public health centers, and hospital outpatients units, etc. To meet this need, our research group is currently developing a multiple vital-signs based infection screening system that can perform human medical inspections within 15 seconds. This system remotely monitors facial temperature, heart and respiration rates using a thermopile array and a 24-GHz microwave radar, respectively. In this work, we redesigned our previous system to make a higher performance with a user-friendly interface. Moreover, the system newly included a multivariable logistic regression model (MLRM) to determine the possibility of infection. We tested the system on 34 seasonal influenza patients and 35 normal control subjects at the Japan Self-Defense Forces Central Hospital. The sensitivity and specificity of the screening system using the MLRM were 85.3% and 88.6%, respectively. © 2014 IEEE.


PubMed | University of Toronto, Saitama University and Lifetech Co.
Type: | Journal: Clinica chimica acta; international journal of clinical chemistry | Year: 2015

Currently molecular techniques are a broadly accepted tool for diagnosis and are able to benefit patients in clinical practice. The polymerase chain reaction (PCR) has been especially incorporated into practical applications that are already in widespread use across the globe. With regard to the initial DNA extraction from clinically relevant samples, a number of commercially available kits are commonly used and are also designed to be easy to handle and less labor-intensive. In this study, the pressure system extracting DNA in column-based kit was developed, and its utility was compared with the centrifuge method using sputum from patients who were diagnosed with pneumonia. Also, due to the compact size and rapid processing time, the practical application of the pressure-based system incorporated into an automated pipetting machine was evaluated through clinical study. Our data suggests that DNA extraction by pressure was capable of serving as a substitute for the centrifuge method, and the compact and automatic nature of the pressure system device provided rapid and valuable information for clinical practice.


Ando K.,Tokyo Denki University | Uchida H.,Saitama University | Ahmed S.,Shahjalal University of Science and Technology | Nishigaki K.,Saitama University | And 2 more authors.
Artificial Life and Robotics | Year: 2014

In this paper, we propose a position detection method for a microwell with manageable volume (MMV) chip on the stage of the inverted microscope. When the manipulation robot arm puts an MMV chip on the microscope stage, there is alignment error up to 500 μm. This error affects measurement accuracy of fluorescence from solution filled in a tiny hall of the MMV chip. Usually, CCD image processing is used for detecting a position. However, appending a CCD camera to our microscope increases the complexity of the optical system. Therefore, we used a photon multiplier tube which was already equipped for measuring fluorescence. In the result, the position of the MMV chips was detected by putting a pilot LED directly above the objective lens. The detection error was less than 10 μm. Although this method was devised for our system, we considered that it was available in other micro optics systems. © 2014 ISAROB.


Sun G.,Tokyo Metroplitan University | Vinh N.Q.,Tokyo Metroplitan University | Abe S.,Takasaka Clinic | Takei O.,Lifetech CO. | And 2 more authors.
International Journal of E-Health and Medical Communications | Year: 2013

After outbreak of severe acute respiratory syndrome (SARS) in 2003, many international airport quarantines adopted fever-based screening to identify infected individuals using infrared thermography to control global pandemic. Unfortunately, the sensitivity of fever-based screening system did not exceed 70.4% at Narita International Airport. In order to achieve accurate onboard entry screening for highly contagious infectious diseases, the authors developed a portable system designed for onboard entry screening with linear discriminant analysis. Within several tens of seconds, the system automatically discriminates infected individuals from normal subjects using measured heart rate, respiratory rate, as well as facial surface temperature determined by thermography. The size of system is small enough to be placed on airplane tray tables. The authors tested on 68 subjects including 12 influenza patients to evaluate the system. The result showed sensitivity of 91.7% and specificity of 92.9%. The system seems to be promising for onboard infection screening to safeguard public health. Copyright © 2013, IGI Global.


Sun G.,Tokyo Metroplitan University | Abe S.,Takasaka Clinic | Takei O.,Lifetech. Co. | Matsui T.,Tokyo Metroplitan University
Proceedings - International Conference on Instrumentation, Communication, Information Technology and Biomedical Engineering 2011, ICICI-BME 2011 | Year: 2011

We developed a portable screening system designed for onboard entry screening at international airports. The system can identify passengers who are potentially infected with epidemic diseases such as severe acute respiratory syndrome (SARS) or pandemic influenza. With its size being small enough to be placed on airplane tray tables, the system enables entry screening while passengers are onboard. In addition, this automatic system may greatly reduce time and effort required for screening. © 2011 IEEE.


Sun G.,Tokyo Metroplitan University | Matsui T.,Tokyo Metroplitan University | Kim S.,Tokyo Metroplitan University | Takei O.,LIFETECH Co.
2014 IEEE 3rd Global Conference on Consumer Electronics, GCCE 2014 | Year: 2014

The outbreak of infectious diseases such as swine flu (H1N1) and avian flu (H5N1) are threatening global health. Especially, developing countries in the South-East Asia region have been at serious risk. Rapid and reliable screening methods are urgently needed for preventing the spread of infection at mass gathering places such as, airport quarantine facilities and hospital outpatient units. In order to meet this need, we developed a multiple vital-signs based infection screening system (KAZEKAMO) that can perform medical inspections within 15 seconds. The system monitors not only body temperature but also heart and respiration rates. By using these three vital-signs, the detection accuracy of the system improved significantly. © 2014 IEEE.

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