Shankar S.,Life Teck Research Center |
Kansrajh C.,Life Teck Research Center |
Dinesh M.G.,Life Teck Research Center |
Satyan R.S.,Life Teck Research Center |
And 2 more authors.
International Journal of Environmental Science and Technology | Year: 2014
Bioremediation of oil spillage in soils using consortia of microbes beckons much exploration. The present study involves bioremediation of oil-contaminated soils from north Chennai, India, using indigenous microbial consortia. Totally, 32 positive oil degrading isolates were obtained from 3 different locations, i.e., petrol filling stations, automobile workshops and oil refineries. Substrate utilization patterns of individual isolates and the consortial sets were observed. Mixture of three common hydrocarbons (petrol, diesel and engine oil) was used for studies. The substrate oil utilized by consortia was taken for thin-layer and column chromatography which perfectly resulted in varied fractions of oil compared to the unused oil as control. The best consortia were used directly for bioremediation experiment. Three different oil-contaminated soils were used and bioremediation patterns were observed. The rate of bioremediation differed within soils, nevertheless all soils were almost 100 % reclaimed within 30 days. Bioremediation kinetics showed that the process corresponds to first-order kinetics and kinetic constants for the different soils ranged from 0.051 to 0.077/day. Assessment of detoxification of acute phytotoxicity owing to the pollutant oil was done, and results observed were significant. An increase of 25, 300 and 212 % in germination index, average growth index and sustenance index, respectively, of Trigonella foenum-graecum Linn. in treated soils was observed, compared to untreated soils. Thus, this study confirmed that microbes in 'Consortial Union' serve as better treating agents in bioremediation of oil-contaminated soils than individual microorganisms. © 2013 Islamic Azad University (IAU).
Rajalakshmi S.,CSIR - Central Leather Research Institute |
Weyhermuller T.,Max Planck Institute for Chemistry |
Dinesh M.,Life Teck Research Center |
Nair B.U.,CSIR - Central Leather Research Institute
Journal of Inorganic Biochemistry | Year: 2012
Two copper(II) complexes with terpyridyl conjugates, [Cu(meotpy)(dmp)] (NO3)2 (1) and [Cu(bitpy)(dmp)](NO3) 2 (2) where meotpy, bitpy and dmp stand for methoxybenzyl terpyridine, benzimidazolyl terpyridine and dimethyl phenanthroline respectively have been synthesized and characterized. Complex 1 has also been characterized crystallographically. Both the complexes have been found to bind CT-DNA intercalatively. The ability of these complexes to bring about DNA cleavage has been analyzed using gel electrophoresis. Both complexes 1 and 2 have been found to bring about hydrolytic cleavage of DNA. The cytotoxicity of both these complexes has been tested against cancerous as well as non-cancerous cell lines. Towards non-cancerous cell line complex 2 exhibited very low toxicity. On the other hand both the complexes have been found to exhibit cytotoxic effects against cancerous cell lines. Complex 2 which has lower IC50, was found to be a potent antiproliferative agent against MCF-7 cells and was able to induce mitochondrial-mediated and caspase-dependent apoptosis with increase in G0/G1 and subsequent arrest in the S phase, in cell cycle progression. Based on this study, it is hypothesized that 2 may be a suitable candidate for further evaluation as a chemopreventive and chemotherapeutic agent for human cancer. © 2012 Elsevier Inc. All rights reserved.
PubMed | Life Teck Research Center
Type: Journal Article | Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] | Year: 2013
The present study was conducted to determine the virulence and cytotoxicity of Aeromonas hydrophila strains isolated from seafood samples collected from 5 major fish markets in Chennai, Tamil Nadu, India. Among 73 A. hydrophila strains isolated from fish and shrimp samples, 86.3% exhibited haemolysis, 78.1% produced slime, 98.63% produced protease and also demonstrated cytotoxicity on Vero cells. Cell shrinkage, detachment and rounding of Vero cells were recorded as cytotoxic changes. Only one strain did not show haemolysis, slime production, proteolytic activity and cytotoxicity on treatment with Vero cells. Positive correlation was observed between proteolytic activity and cytotoxicity irrespective of haemolytic activity of the strains. These results demonstrated the presence of wide spread, pathogenically characterized, cytotoxic seafood borne A. hydrophila in Chennai.