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Fushimi T.,Kyoto University | Miura N.,Kyoto University | Shintani H.,Kyoto University | Tsunoda H.,Life Science Research Laboratory | And 2 more authors.
Applied Microbiology and Biotechnology | Year: 2013

Pyrosequencing system utilizing luciferase is one of the next-generation DNA sequencing systems. However, there is a crucial problem with the current pyrosequencing system: luciferase cannot discriminate between ATP and dATP completely, and dATPαS must be used as the dATP analogue. dATPαS is expensive and has low activity for the enzyme. If luciferase can clearly recognize the difference between ATP and dATP, dATP could be used instead of the expensive dATPαS in the pyrosequencing system. We attempted to prepare a novel luciferase with improved specific activity and dATP discrimination with the molecular display method. First, we selected two amino acid residues, Ser440 and Ser456, as target residues for mutation from the whole sequence of Photinus pyralis luciferase; we comprehensively mutated these two amino acids. A mutant luciferase library was constructed using yeast cell surface engineering. Through three step-wide screenings with individual conditions, we easily and speedily isolated three candidate mutants from 1,152 candidates and analyzed the properties of these mutants. Consequently, we succeeded in obtaining interesting mutant luciferases with improved specific activity and dATP discrimination more conveniently than with other methods. © Springer-Verlag Berlin Heidelberg 2012.


Yamashita K.,Shikoku Chemicals Corporation | Ide H.,Shikoku Chemicals Corporation | Asada H.,Shikoku Chemicals Corporation | Narita Y.,Life Science Research Laboratory | Nozaki A.,Tohoku Bunka Gakuen University
Indoor Air 2014 - 13th International Conference on Indoor Air Quality and Climate | Year: 2014

The porous raw materials such as zeolite, high-grade charcoal produced from ubame oak, diatomaceous soil are noted as a moisture and pollutant absorbing materials. In this project experiments were carried out to quantify the adsorption properties of both the porous raw materials and interiors plaster using the porous raw materials. The experiments were peformed both small and large chambers. In a small chamber, the capability of porous materials to abosorb formaldehyde and VOC(toluene) was confirmed. In a large chamber similar to real-world environments, the evaluation of the effectiveness of walls treated with diatomaceous soil to adsorb formladehyde and ammonia confirmed storng capacity. These suggest that the use of diatomaceous soil and other porous materials in treating walls could be effective in reducing the concentration of harmful chemical compounds inside rooms.


Tanaka T.,Daikin Industries Ltd | Kagawa K.,Daikin Industries Ltd | Narita Y.,Life Science Research Laboratory | Nozaki A.,Tohoku Bunka Gakuen University
Indoor Air 2014 - 13th International Conference on Indoor Air Quality and Climate | Year: 2014

In this study, we suggest that the performance of a filter media cannot always represent the performance of the equipment containing that filter in the real condition of use. We evaluated the gas removal performances by the different three ways; a) one-pass removal performance of the filter media itself: Eo, b) one-pass removal performance of the air purifier equipment: Ee, and c) overall gas removal performance in the real scale chamber by the use the of air purifier equipment: Ec. The result was Eo > Ee > Ec. It is inferable that the actual performance as an air purifier cannot be shown by the performance of the filter because of the ununiform air flow within the air purifier and the ununiformity of gas concentration in the chamber.


Gaikwad S.,Life Science Research Laboratory | Gore R.,Life Science Research Laboratory | Garad K.,Life Science Research Laboratory | Gaikwad S.,Shivaji College | Mulani R.,Dr. Babasaheb Ambedkar Marathwada University
Check List | Year: 2015

The geophytes in the northern Western Ghats are a significant component of the herbaceous flora, both in terms of abundance and diversity, and comprise approximately 10% of plant species. About 186 geophytic taxa were here recorded from northern Western Ghats of India during the present work. Of them, 62 geophytes are known only from northern Western Ghats. At least 22 plant families exhibit this life form. In the study region, geophytes have adapted to a monsoon seasonality. They sprout new foliage with the onset of monsoon rain in June and complete their life cycle in October when monsoon rains halt. Many of them produce flowers during the vegetative growing season but some charismatically flowers in summer season (April–May), often without leaves. A few taxa have abandoned their geophytic habit and grow in seasonal water bodies, and possess leaves without stomata and non-buoyant seeds. © 2015 Check List and Authors.


Gaikwad S.,Life Science Research Laboratory | Gore R.,Life Science Research Laboratory | Garad K.,Life Science Research Laboratory | Gaikwad S.,Shivaji College
Check List | Year: 2014

A checklist of endemic flowering plant species of the northern Western Ghats (Sahyadri Ranges) of India is provided along with their IUCN threatened category. There are 159 flowering plant taxa belonging to 81 genera and 31 families found to be strictly endemic to the Sahyadri Ranges. The genus Ceropegia has the largest number (17) of endemic species. Five monotypic genera are restricted to the Sahyadri Ranges. Most of these endemic taxa are restricted to small biogeographical areas and are rare in occurrence. Field assessment has shown that 34 endemic taxa fall into IUCN category Critically Endangered, 18 into Endangered and 20 into Vulnerable. A large number of endemic taxa (34) are known only by their type collection, which could not be recollected even after repeated field explorations undertaken by several workers in their habitat of occurrence in last two decades. Hence, they are put in the category ‘Data Deficient’. © 2014 Check List and Authors.

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