Life Science Research Laboratory

Solāpur, India

Life Science Research Laboratory

Solāpur, India
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Gaikwad S.,Life Science Research Laboratory | Gore R.,Life Science Research Laboratory | Garad K.,Life Science Research Laboratory | Gaikwad S.,Shivaji College
Check List | Year: 2014

A checklist of endemic flowering plant species of the northern Western Ghats (Sahyadri Ranges) of India is provided along with their IUCN threatened category. There are 159 flowering plant taxa belonging to 81 genera and 31 families found to be strictly endemic to the Sahyadri Ranges. The genus Ceropegia has the largest number (17) of endemic species. Five monotypic genera are restricted to the Sahyadri Ranges. Most of these endemic taxa are restricted to small biogeographical areas and are rare in occurrence. Field assessment has shown that 34 endemic taxa fall into IUCN category Critically Endangered, 18 into Endangered and 20 into Vulnerable. A large number of endemic taxa (34) are known only by their type collection, which could not be recollected even after repeated field explorations undertaken by several workers in their habitat of occurrence in last two decades. Hence, they are put in the category ‘Data Deficient’. © 2014 Check List and Authors.


Kitano S.,Life Science Research Laboratory | Nakayama M.,Life Science Research Laboratory | Yamane A.,Life Science Research Laboratory | Tsukahara Y.,RIKEN | Amano M.,Life Science Research Laboratory
Analytical Biochemistry | Year: 2011

Molecularly targeted agents for cancer therapy are recognized as being effective and are gaining in popularity. However, the efficacy of the agents depends on the status of the targeted molecule such as the number of molecules expressed, activity, and mutation. Therefore, the use of companion diagnostics for investigating the status of the targeted molecule prior to therapy is highly important. We developed a simple and cost-effective somatic mutation detection method called the fluorescence resonance energy transfer-based preferential homoduplex formation assay (FRET-PHFA). By using double-stranded labeled DNA and fluorescence measurement with thermal control, this method provides higher reproducibility, easier handling, less risk for contamination, shorter assay time (only ~15. min), and less cost compared with conventional PHFA. Here we report the evaluation of FRET-PHFA on the detection of multiallelic KRAS mutations in codons 12 and 13 compared with the TheraScreen clinical diagnostics kit. We found that FRET-PHFA detected KRAS mutations (1.25-50%) from all cell line DNA titration samples. © 2010 Elsevier Inc.


Gore R.D.,Life Science Research Laboratory | Garad K.U.,Life Science Research Laboratory | Gaikwad S.P.,Life Science Research Laboratory
Rheedea | Year: 2014

Crypsis aculeata (L.) Ait. is reported for the first time from Peninsular India. It occurs on mud in drying lake near Osmanabad town of Maharashtra state. A detailed description, illustration and key to the Indian species of Crypsis Ait. are provided here.


Gaikwad S.P.,Life Science Research Laboratory | Gore R.D.,Life Science Research Laboratory | Garad K.U.,Life Science Research Laboratory
Rheedea | Year: 2012

Astraea lobata (L.) Klotzsch (Euphorbiaceae) is recorded for the first time from India. It occurs on roadsides near Akkalkot in the Solapur district of Maharashtra. A detailed description with illustration of the species is provided here.


Gaikwad S.,Life Science Research Laboratory | Gore R.,Life Science Research Laboratory | Garad K.,Life Science Research Laboratory
Check List | Year: 2014

A detailed inventory of all indigenous and exotic species of trees from the Balaghat Ranges of Maharashtra, India is provided. About 228 tree taxa belonging to 44 families and 132 genera have been recorded from the study region, of which 174 are indigenous and 53 are exotic. The families Fabaceae, Malvaceae, Bignoniaceae, Rubiaceae and Moraceae are dominant in the arborescent flora. Acacia Mill. is the largest genus with 19 tree taxa. Vegetation type and the months of flowering and fruiting are given for each tree taxon. Trees of Balaghat Ranges have many potential uses like sources of food, fodder, timber, fuel wood, dye, essential oils, medicines etc. However, forests of Balaghat Ranges have been declining due to anthropogenic activities and tourism. Hence, the present study was undertaken to generate baseline data, which will prove helpful for the conservation and protection the forest of Balaghat Ranges. © 2014 Check List and Authors.


Fushimi T.,Kyoto University | Miura N.,Kyoto University | Shintani H.,Kyoto University | Tsunoda H.,Life Science Research Laboratory | And 2 more authors.
Applied Microbiology and Biotechnology | Year: 2013

Pyrosequencing system utilizing luciferase is one of the next-generation DNA sequencing systems. However, there is a crucial problem with the current pyrosequencing system: luciferase cannot discriminate between ATP and dATP completely, and dATPαS must be used as the dATP analogue. dATPαS is expensive and has low activity for the enzyme. If luciferase can clearly recognize the difference between ATP and dATP, dATP could be used instead of the expensive dATPαS in the pyrosequencing system. We attempted to prepare a novel luciferase with improved specific activity and dATP discrimination with the molecular display method. First, we selected two amino acid residues, Ser440 and Ser456, as target residues for mutation from the whole sequence of Photinus pyralis luciferase; we comprehensively mutated these two amino acids. A mutant luciferase library was constructed using yeast cell surface engineering. Through three step-wide screenings with individual conditions, we easily and speedily isolated three candidate mutants from 1,152 candidates and analyzed the properties of these mutants. Consequently, we succeeded in obtaining interesting mutant luciferases with improved specific activity and dATP discrimination more conveniently than with other methods. © Springer-Verlag Berlin Heidelberg 2012.


Tanaka T.,Daikin Industries Ltd | Kagawa K.,Daikin Industries Ltd | Narita Y.,Life Science Research Laboratory | Nozaki A.,Tohoku Bunka Gakuen University
Indoor Air 2014 - 13th International Conference on Indoor Air Quality and Climate | Year: 2014

In this study, we suggest that the performance of a filter media cannot always represent the performance of the equipment containing that filter in the real condition of use. We evaluated the gas removal performances by the different three ways; a) one-pass removal performance of the filter media itself: Eo, b) one-pass removal performance of the air purifier equipment: Ee, and c) overall gas removal performance in the real scale chamber by the use the of air purifier equipment: Ec. The result was Eo > Ee > Ec. It is inferable that the actual performance as an air purifier cannot be shown by the performance of the filter because of the ununiform air flow within the air purifier and the ununiformity of gas concentration in the chamber.


Gaikwad S.P.,Life Science Research Laboratory | Garad K.U.,Life Science Research Laboratory | Gore R.D.,Life Science Research Laboratory
Kew Bulletin | Year: 2014

A new species, Crinum solapurense S. P. Gaikwad, K. U. Garad & R. D. Gore, is described from a swamp bordering the Bhima River in Solapur district of Maharashtra State, India. It resembles C. viviparum (Lam.) R. Ansari & V. J. Nair var. viviparum and C. lorifolium Roxb. ex Ker Gawl. but differs in having 1 - 10 bulblets on the mother bulb, sturdy and canaliculate 12 - 27 leaves, 10 - 30-flowered umbels, undivided stigma and 3 - 12-seeded fruits. © 2014 The Board of Trustees of the Royal Botanic Gardens, Kew.


PubMed | Life Science Research Laboratory
Type: | Journal: Biodiversity data journal | Year: 2015

The present paper provides an enumeration of leguminous taxa of Balaghat Ranges of Maharashtra along with their habits, phenological deta and voucher specimen numbers. During the present work, a total of 123 species, 4 subspecies and 17 varieties of Fabaceae have been recorded for Balaghat Ranges of Maharashtra, of which 119 taxa are occurring in wild while 25 are under cultivation. The members of Fabaceae are dominant in herbaceous vegetation of the Balaghat Ranges. There are more species in genera like Crotalaria (23 taxa), Indigofera (16 taxa), Alysicarpus (14 taxa), Vigna (11 taxa) and Desmodium (8 taxa). Twelve taxa are endemic to India of which Indigoferadeccanensis falls into Critically Endangered IUCN Red data category. The legumes of Balaghat Ranges have many actual and potential uses such as food, fodder and sources of edible oil, natural dyes, industrial lubricants, timber and medicines. About 19 leguminous taxa are wild relatives of food and fodder crops have resistance to pests and diseases, and abiotic stresses such as drought and salinity, can be used for crop improvement.


PubMed | Life Science Research Laboratory
Type: | Journal: Biodiversity data journal | Year: 2015

Vigna subg. Ceratotropis (Piper) Verdc. represents a homogenous and distinct group of species with highly specialized complex floral characters. It is most diverse in Asia. India, with 24 species, represents a secondary center of species diversity of the subgenus.A new species, Vignapandeyana RD Gore, SP Gaikwad & SD Randive, is described from hill slopes of the northern Western Ghats of India. It resembles Vignayadavii Gaikwad et al. and Vignadalzelliana (Kuntze) Verdc. but differs from the latter in its dimorphic shoots (some subterranean, with cleistogamous flowers) and densely hairy pods, from the former by its curved style, flattened style beak, foveolate seed coat and absence of standard protuberance and horn-like keel pocket in cleistogamous flowers.

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