Heo Y.J.,Tokyo University of Science |
Heo Y.J.,Life Bio Electromechanical Autonomous Nano Systems Center Project |
Takahashi M.,Life Bio Electromechanical Autonomous Nano Systems Center Project |
Takahashi M.,TERUMO Co. RandD Headquarters |
And 9 more authors.
Micro and Nano Letters | Year: 2012
Polyacrylamide (PAM) hydrogels are widely used in bioanalysis and biosensing applications. Scaling down of PAM patterns to micro/nanosize extends PAM applications to lab-on-a-chip, highly sensitive biosensors and cell/tissue analysis. Proposed is a replica moulding technique to pattern the PAM surface down to nanosize. Various patterns on silicon moulds successfully transferred to PAM with a minimum dimension of 60 nm and aspect ratio of up to ∼9. PAM is characterised as a platform for a single cell array and the analysis of cell responses to topographic cues. Moreover, micro/nanopatterned PAM samples were implanted under the back skin of rats to study the topological effects on immuno responses in vivo. Therefore the use of micro/nanoreplica moulding to pattern PAM hydrogel provides opportunities for studying the relationship between soft nanoscaled structures and cell/tissue. © 2012 The Institution of Engineering and Technology.