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Wang Q.,Shanxi Agricultural University | Shi L.,Shanxi Agricultural University | Ren Y.,Shanxi Agricultural University | Yue W.,Shanxi Agricultural University | And 2 more authors.
Animal Reproduction Science | Year: 2012

In this experiment the effect of maternal dietary selenium on the expression of Sel P and apoER2 of goat offspring was studied. The experiment was conducted on 119 Taihang Black Goats randomly divided into 4 groups which were fed with a basal diet, supplemented with 0 (control), 0.5, 2 and 4mgkg-1 DM Se. Testis samples were collected from young male of each treatment group at the end of the study (30d after weaning) for mRNA expression using real-time PCR and for protein expression by immunohistochemistry assay. A significant decrease was observed in mRNA expression of Sel P and apoER2 in the testis of the Se-deficient (Group 1) and the Se-excess (Group 4) compared with that in Groups 2 and 3. A similar trend of the protein expression of Sel P and apoER2 was also found. These data indicate that maternal and dietary selenium has an effect on the expression of Sel P and apoER2 in testis of their offspring. In addition, both groups were similar suggesting that the relationship between Sel P and apoER2, and apoER2 is a receptor of Sel P in the seminiferous epithelium to uptake the selenium. © 2012 Elsevier B.V. Source


Shi L.,Shanxi Agricultural University | Zhang C.,Shanxi Agricultural University | Yue W.,Shanxi Agricultural University | Zhu X.,Shanxi Agricultural University | Lei F.,Licheng Breeding Goat Center
Animal Feed Science and Technology | Year: 2010

The objective of this study was to evaluate the short-term effect of dietary selenium (Se)-enriched yeast on semen parameters in goat during the breeding season. Also, the antioxidant status and Se content in seminal plasma was investigated. A total of 72 adult Taihang black goats of proven fertility were randomly assigned to four treatment groups. They were fed the basal diet supplemented with 0 (control), 0.5, 1.0 and 2.0 mg of Se/kg dry matter (DM) (from Se-enriched yeast). Semen was collected by an artificial vagina (AV) and semen characteristics (volume, motility, viability, concentration and abnormality) were recorded. Seminal plasma was harvested by centrifugation for analyzing enzyme activities and Se concentration. The result showed that short-term dietary Se supplementation has both linear (P<0.001) and quadratic (P<0.001) effects on semen parameters and antioxidant activities. It could be concluded that the short-term supplementation of Se-enriched yeast can improve semen quality in goat and Se plays an important role in oxidative systems of seminal plasma. The results of this study provide an effective approach to improve the reproductive performance at a lower feeding cost through short-term dietary supplementation of organic Se. © 2010 Elsevier B.V. All rights reserved. Source


Ren Y.,Shanxi Agricultural University | Wang Q.,Shanxi Agricultural University | Shi L.,Shanxi Agricultural University | Yue W.,Shanxi Agricultural University | And 2 more authors.
Animal Reproduction Science | Year: 2011

The aim of the study was to evaluate the effect of selenium on the expression of p34cdc2 and CyclinB1 (two components of MPF regulating cell cycle) of germ cells of their offspring in goats. A herd of 119 Taihang Black Goats, which was randomly divided into 4 treatments, received experimental diet with different Se levels (from Se-enriched yeast) for 174d. The four treatments, fed with a basal diet, were supplemented with 0(control), 0.5, 2 and 4mgkg-1 DM Se. Testis samples were collected from the young male goats of each treatment group at the end of the study (30d after weaning) for mRNA expression using real-time PCR and for protein expression by immunohistochemistry assay. Results show that a significant decrease was observed in mRNA expression of p34cdc2 and CyclinB1 in the testis of Se-deficient (Group 1) and Se-excess (Group 4) animals compared with that in Groups 2 and 3. However, no significant changes were found in mRNA expression of p34cdc2 between Se-deficient (Group 1) and Se-excess (Group 4). Also the immunohistochemistry assay detected similar results of protein expression of these two genes. These results suggest, that maternal and dietary Se-induced oxidative stress can modulate the mRNA and protein expression of the cell cycle related genes (p34cdc2 and CyclinB1) in the testis of their offspring. In addition, Se deficiency and Se excess could prevent the completion of the cell cycle. © 2011 Elsevier B.V. Source


Shi L.,Shanxi Agricultural University | Yue W.,Shanxi Agricultural University | Zhang C.,Shanxi Agricultural University | Ren Y.,Shanxi Agricultural University | And 4 more authors.
Animal Reproduction Science | Year: 2010

To investigate the effect of maternal and dietary selenium on antioxidant status in testis and apoptosis of germ cells during spermatogenesis of their offspring, selected Taihang Black Goats (n = 119) were randomly allotted to four treatment groups. They were fed the experimental diet with different Se levels (from Se-enriched yeast) for 174d from 60d prior to lactation to weaning of kids. The treatments were: (1) Group 1 (control), basal diet without Se supplementation, (2) Group 2, the same basal diet supplemented 0.5 mg Se/kg DM, (3) Group 3, the same basal diet supplemented 2 mg Se/kg DM and (4) Group 4, the same basal diet supplemented 4 mg Se/kg DM. Thirty days after weaning, testis samples of the young male goats were collected for mRNA expression and analyzing the antioxidant status and Se concentration, as well as the population of apoptotic germ cells by TUNEL assay. The results show that mRNA expression of apoptosis genes (Bcl-2, Bax and Caspase 8) were significantly higher in Groups 1 and 4 than that in Groups 2 and 3. The same trend was observed in the population of apoptotic cells analyzed by TUNEL assay. GSH-Px activity and Se concentration in testis of offspring was progressively increased with the increasing Se level in diet of dams. However, there was no significant difference in GSH-Px activity between Groups 3 and 4. The lowest MDA content was obtained in Group 2 and a significant decrease was observed in Groups 1, 3 and 4. These data suggest that doe maternal and dietary Se could influence antioxidant status in the testis of their offspring and the oxidative stress related to Se from the dam could modulate mRNA expression of apoptosis genes and apoptosis of germ cells during spermatogenesis. It is possible that Se supplementation of the dam's diet during gestation and lactation could be a way to supply the Se necessary for normal development of reproductive function of their offspring. © 2010 Elsevier B.V. All rights reserved. Source


Wang Q.,Shanxi Agricultural University | Zhang C.,Shanxi Agricultural University | Ren Y.,Shanxi Agricultural University | Yue W.,Shanxi Agricultural University | And 2 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

Selenoprotein P (Seppl) is a protein of considerable intrigue due to its unusual composition and requirements for its biosynthesis. Most selenoproteins contained a single selenocysteine residue, the human, bovine and rodent selenoprotein P genes encode proteins contained 10±12 selenocysteines. In this study, a full-length cDNA was cloned and characterized from Taihang black goat testes. The full-length of Seppl (1511 bp) cDNA contained an open reading frame encoding 347 amino acids. Sequence analysis showed that the predicted Seppl amino acids shared high identities with that of other species. The order of Seppl mRNA expression levels was: liver>lung>spleen>kidney, heart, testes, caput epididymidis and corpus epididymidis. © Medwell Journals, 2012. Source

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