Liaoning Provincial Tumor Hospital

Shenyang, China

Liaoning Provincial Tumor Hospital

Shenyang, China
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Wang W.,Liaoning Provincial Tumor Hospital | Zhang L.,Liaoning Provincial Tumor Hospital | Zheng K.,Liaoning Provincial Tumor Hospital | Zhang X.,Liaoning Provincial Tumor Hospital
Oncology Reports | Year: 2016

MicroRNA-17-5p has been proven upregulated in many human malignancies and correlated with tumor progression. However, its expression and clinical significance in osteosarcoma is still unclear. Thus, the aim of the present study was to explore the effects of miR-17-5p in osteosarcoma tumorigenesis and development. The expression level of miR-17-5p was quantified by quantitative real-time reverse-transcriptasepolymerase chain reaction in primary osteosarcoma tissues and osteosarcoma cell lines. MTT, Transwell and matrigel assays were used to test the proliferation, migration and invasion of miR-17-5p transfection osteosarcoma cells, and a mouse model was used to investigate tumorigenesis. The expression levels of miR-17-5p in osteosarcoma tissues were significantly higher than those in corresponding non-cancerous bone tissues. In addition, miR-17-5p upregulation more frequently occurred in osteosarcoma specimens with advanced clinical stage, positive distant metastasis and poor response to neoadjuvant chemotherapy. After miR-17-5p transfection, cell proliferation, migration, invasion and tumorigenesis in the osteosarcoma cells were significantly promoted. We further demonstrated that BRCC2 is a direct target of miR-17-5p. These findings indicate that miR-17-5p may act not only as a novel diagnostic and prognostic marker, but also as a potential target for molecular therapy of osteosarcoma.


Hua X.-D.,Liaoning Provincial Tumor Hospital | He Z.-Y.,Liaoning Provincial Tumor Hospital
Chinese Journal of Oncology | Year: 2012

Objective: To evaluate the therapeutic efficacy of sorafenib in combination with microwave coagulation therapy (MCT) and trans-arterial chemoembolization (TACE) in patients with recurrent liver cancer. Methods: From January 2006 to January 2010, 90 patients with recurrent hepatocellular carcinoma (HCC) were treated with MCT and TACE in our hospital. The treatment group received sorafenib + MCT + TACE, and the control group received MCT + TACE. Results: RR of the treatment group was 66.7% , which of the control group was 52.0% (P > 0.05). DCR was 83.3% in the treatment group and 64.5% in the control group (P < 0.05). Through a comparison of survival curves along with the extension of time, the survival rates of the two groups were decreased, but the treatment group (group 1) had a significantly higher one than the control group (group 2), with a statistically significant difference (P > 0.05). Conclusion: Sorafenib combined with MCT and TACE can improve the disease control rate and prolong the survival in patients with recurrent HCC.


Lin T.,Liaoning Provincial Tumor Hospital | Song C.,Liaoning Provincial Tumor Hospital | Wang H.,Liaoning Provincial Tumor Hospital
Chinese Journal of Clinical Oncology | Year: 2014

Objective: This study aimed to observe the long-term effect of Pseudomonas aeruginosa preparation used in peritoneal injection of advanced colorectal cancer patients during surgery. Methods: A total of 83 colorectal cancer patients who received surgery between September 2006 and March 2008 were enrolled in this study. The patients were divided into two groups. Palliative resection and a 10 ml P. aeruginosa peritoneal injection were performed in 30 of 83 patients in the treatment group. Simple palliative resection was conducted in the other 53 patients, which comprised the control group. Both groups were then treated by regular chemotherapy and radiotherapy. Results: The follow-up visit was completed in 79 of 83 patients, with a high follow-up rate of 95.2%. No significant difference was found in the five-year overall survival time between the two groups (P=0.403). However, the five-year median survival time in the control group was only 13.9 ± 2.14 months, whereas that in the treatment group was 17.2 ± 2.12 months. Conclusion: Within a short period, peritoneal injection of P. aeruginosa during surgery could confer certain survival advantages for advanced colorectal cancer patients. However, the long-term effect of this therapy remains unknown.


Yang H.,Liaoning Provincial Tumor Hospital | Shen X.,Liaoning Provincial Tumor Hospital | Li B.,Liaoning Provincial Tumor Hospital | Ma R.,Liaoning Provincial Tumor Hospital
Tumor Biology | Year: 2014

The relationship between glutathione S-transferase T1 (GSTT1) gene polymorphism and the risk of lung cancer from the published reports are still conflicting. This study was conducted to evaluate the relationship between GSTT1 polymorphism and the risk of lung cancer. A comprehensive research was conducted through the databases, and 55 studies were recruited into this meta-analysis for the association of null genotype of GSTT1 with lung cancer susceptibility, consisting of 15,140 patients with lung cancer and 16,662 controls. There was a significant association between GSTT1 null genotype and lung cancer risk in the overall populations (OR = 1.138, 95 % CI = 1.032-1.255, P heterogeneity = 0.000, P = 0.009). Furthermore, GSTT1 null genotype was associated with the lung cancer risk in Asians (OR = 1.469, 95 % CI = 1.228-1.757, P heterogeneity = 0.000, P = 0.000). However, GSTT1 null genotype was not associated with the risk of lung cancer in Caucasians and Africans. In conclusion, GSTT1 null genotype is associated with the lung cancer in overall populations and in Asians. © 2013 International Society of Oncology and BioMarkers (ISOBM).


Hao Z.-Q.,Liaoning Provincial Tumor Hospital | Fu X.-B.,Liaoning Provincial Tumor Hospital | Hua X.-D.,Liaoning Provincial Tumor Hospital | Fu Q.-C.,Liaoning Provincial Tumor Hospital
National Medical Journal of China | Year: 2012

Objective: To explore the diagnosis and treatment of focal nodular hyperplasia (FNH) of liver. Methods: The clinical data were retrospectively analyzed for 26 cases with confirmed FNH of liver from January 2006 to July 2011. Enhanced computed tomography and magnetic resonance imaging were performed. Results Among them, 22 cases underwent surgical resection, including left hemihepatectomy (n=4), left lateral lobe hepatectomy (n=5) and partial hepatectomy (n=13). The pathological diagnosis was FNH. Most tumors were of soft texture. The gross surface was brown or yellow-brown in color. Central scar and radiating fibrous septas were spotted in some cases. There was no recurrence during a follow-up period of 4 months to 5 years. Serial observations were conducted for 4 cases with a follow-up period of 2-4 years. No growth was observed. Conclusions: Enhanced CT and MRI are important diagnostic tools. The confirmed cases may be followed up. Surgical resection is effective with an excellent prognosis.


Liu B.,Liaoning Provincial Tumor Hospital | Xu K.,Liaoning Provincial Tumor Hospital | Han H.-B.,Liaohe Oilfield second Hospital Workers | Wang T.-L.,Liaoning Provincial Tumor Hospital | Song Y.-Q.,Liaoning Provincial Tumor Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2014

OBJECTIVE: To investigate the function of resveratrol (Res) and its mechanism in lung adenocarcinoma A549 cells. METHODS: A549 cells were treated with resveratrol at different concentretions for 24 h and the morphological change was observed by phase contrast microscope. Apoptosis features were observed by fluorescence microscope after nuclear DAPI staining. The proliferation of A549 cells was analyzed using methyl thiazolyl tetrazolium (MTT) assay. The expression of p53, Bax, Bcl-2 and Cleaved-Caspase3 after Res treatment in A549 cells was measured by Western blot. RESULTS: After Res treatment, we found that the interspace of A549 cells was decreased and granular material was found in nucleus. These changes were remarkably associated with the increase of resveratrol concentration. DAPI staining indicated that Res induces apoptosis in A549 cells. After treated with 25-200 μmol/L Res, the proliferation was markedly inhibited with a does dependent manner (25 μmol/L, 12.4%; 50 μmol/L, 21.6%; 100 μmol/L, 51.3%; 200 μmol/L, 89.7%), and IC50 was 98 μmol/L. Otherwise, after Res treatment, the expression of p53, Bax and Cleaved-Caspase3 protein was upregulated (r=0.930, P<0.001; r=0.874, P<0.001), Bcl-2 and the ratio of Bcl-2/Bax were downregulated (r=0.753, P=0.01). CONCLUSION: Resveratrol could inhibit the proliferation and induce apoptosis through p53 method in A549 cells, and the expression of Bax, Bcl-2 and Cleaved-Caspase3 participate in the apoptotic process modulated by resveratrol.


PubMed | Liaoning Provincial Tumor Hospital
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2014

The relationship between glutathione S-transferase T1 (GSTT1) gene polymorphism and the risk of lung cancer from the published reports are still conflicting. This study was conducted to evaluate the relationship between GSTT1 polymorphism and the risk of lung cancer. A comprehensive research was conducted through the databases, and 55 studies were recruited into this meta-analysis for the association of null genotype of GSTT1 with lung cancer susceptibility, consisting of 15,140 patients with lung cancer and 16,662 controls. There was a significant association between GSTT1 null genotype and lung cancer risk in the overall populations (OR=1.138, 95% CI=1.032-1.255, P heterogeneity=0.000, P=0.009). Furthermore, GSTT1 null genotype was associated with the lung cancer risk in Asians (OR=1.469, 95% CI=1.228-1.757, P heterogeneity=0.000, P=0.000). However, GSTT1 null genotype was not associated with the risk of lung cancer in Caucasians and Africans. In conclusion, GSTT1 null genotype is associated with the lung cancer in overall populations and in Asians.


PubMed | Liaoning Provincial Tumor Hospital
Type: Journal Article | Journal: Zhonghua yi xue za zhi | Year: 2012

To explore the diagnosis and treatment of focal nodular hyperplasia (FNH) of liver.The clinical data were retrospectively analyzed for 26 cases with confirmed FNH of liver from January 2006 to July 2011. Enhanced computed tomography and magnetic resonance imaging were performed.Among them, 22 cases underwent surgical resection, including left hemihepatectomy (n = 4), left lateral lobe hepatectomy (n = 5) and partial hepatectomy (n = 13). The pathological diagnosis was FNH. Most tumors were of soft texture. The gross surface was brown or yellow-brown in color. Central scar and radiating fibrous septas were spotted in some cases. There was no recurrence during a follow-up period of 4 months to 5 years. Serial observations were conducted for 4 cases with a follow-up period of 2 - 4 years. No growth was observed.Enhanced CT and MRI are important diagnostic tools. The confirmed cases may be followed up. Surgical resection is effective with an excellent prognosis.


PubMed | Liaoning Provincial Tumor Hospital
Type: Journal Article | Journal: Oncology reports | Year: 2016

MicroRNA-17-5p has been proven upregulated in many human malignancies and correlated with tumor progression. However, its expression and clinical significance in osteosarcoma is still unclear. Thus, the aim of the present study was to explore the effects of miR-17-5p in osteosarcoma tumorigenesis and development. The expression level of miR-17-5p was quantified by quantitative real-time reverse-transcriptase-polymerase chain reaction in primary osteosarcoma tissues and osteosarcoma cell lines. MTT, Transwell and matrigel assays were used to test the proliferation, migration and invasion of miR-17-5p transfection osteosarcoma cells, and a mouse model was used to investigate tumorigenesis. The expression levels of miR-17-5p in osteosarcoma tissues were significantly higher than those in corresponding non-cancerous bone tissues. In addition, miR-17-5p upregulation more frequently occurred in osteosarcoma specimens with advanced clinical stage, positive distant metastasis and poor response to neo-adjuvant chemotherapy. After miR-17-5p transfection, cell proliferation, migration, invasion and tumorigenesis in the osteosarcoma cells were significantly promoted. We further demonstrated that BRCC2 is a direct target of miR-17-5p. These findings indicate that miR-17-5p may act not only as a novel diagnostic and prognostic marker, but also as a potential target for molecular therapy of osteosarcoma.


PubMed | Liaoning Provincial Tumor Hospital
Type: Journal Article | Journal: International immunopharmacology | Year: 2015

Non-small cell lung cancer (NSCLC) is highly prevalent and needs novel therapies. Melanoma-associated antigen 3 (MAGE-A3) is a lung cancer antigen and calreticulin (CALR) can modulate immune responses. Our previous study has shown that up-regulated MAGE-A3 and CALR expression inhibits the proliferation and invasion of glioma cells. In this study, we examined the effect of adenovirus (Ad)-mediated MAGE-A3 and/or CALR expression on the proliferation, invasion, and apoptosis of human NSCLC cells and on the vascular tube formation of human endothelial cells as well as on dendritic cell (DC) activation and induced CD8(+) cytotoxic T lymphocyte (CTL) activity in vitro. We found that low levels of CALR and MAGE-A3 were expressed by A549 cells, but only very low CALR was expressed by DC. Up-regulated CALR and MAGE-A3 expression by infection with Ad-CALR/MAGE-A3 significantly inhibited the proliferation and invasion, but promoted the apoptosis of A549 cells. Up-regulated CALR and MAGE-A3 expression significantly inhibited cyclin D1 expression and the AKT, ERK1/2 and NF-B expression and phosphorylation in A549 cells. Up-regulated CALR expression inhibited the tube formation in human endothelial cells. Up-regulated CALR and MAGE-A3 expression synergistically enhanced classical DC activation by enhancing IL-12, but reducing IL-10 secretion. Furthermore, CTLs induced by up-regulated CALR and MAGE-A3 expressing DCs synergistically triggered A549 cell apoptosis, which was abrogated by treatment with anti-HLA I, but not anti-HLA II antibodies. Moreover, CTLs induced by CALR and MAGE-A3-expressing DCs had a higher frequency of A549-specific IFN--secreting T cells. Our data indicated that up-regulated CALR and MAGE-A3 expression inhibited the carcinogenesis of NSCLC by modulating the AKT, ERK MAPK and NF-B signaling and enhanced classical DC activation and MAGE-A3-specific CTL cytotoxicity. Therefore, our findings may provide new insights in understanding the role of CALR in modulating antigen-specific T cell immunity and may aid in the design of new therapies for NSCLC.

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