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Xu F.,Shenyang University | Dai C.,Shenyang University | Zhang R.,Liaoning Province Tumor Hospital | Zhao Y.,Shenyang University | And 2 more authors.
Digestive Diseases and Sciences | Year: 2012

Background: At present, the relationship between Nanog expression and the biological behavior and prognosis of colorectal cancer is still unclear. Aim: The purpose of this study was to evaluate the expression and regulatory effects of Nanog in colorectal cancer and the correlation between Nanog protein expression and the prognosis of patients with colorectal cancer. Materials and Methods: The differential expression of genes between CD133+ tumor cells and CD133- tumor cells were detected using RT 2 Profiler™ PCR Array. The Nanog mRNA expression level was detected by RT-PCR and the protein level was detected using immunohistochemistry staining. The relationship between Nanog expression and clinicopathological parameters of colorectal cancer was determined. Results: Nanog were expressed significantly higher in CD133+ tumor cells compared to CD133- tumor cells. It was observed that 72 (20.00 %) of the 360 cases positively expressed Nanog. Univariate analyses indicated that Nanog expression was related to histological grade, lymph node metastasis, TNM stage, and liver metastasis (P = 0.005, 0.001, 0.001 and 0.012, respectively). Spearman correlation analysis showed that Nanog expression has a linear correlation to liver metastasis (P = 0.001). After conducting multivariate analysis, histological grade, TNM stage, and Nanog were found to be related to liver metastasis (P = 0.020, 0.01 and 0.001, respectively). In the Cox regression test, the histological grade, Lymph node metastasis, TNM stage, liver metastasis, and Nanog were detected as the independent prognostic factors (P = 0.02, 0.045, 0.01, 0.001 and 0.001, respectively). Conclusions: Nanog protein may be a potential biomarker for postoperative liver metastasis of colorectal cancer. © 2012 Springer Science+Business Media, LLC. Source

Sun T.,Liaoning Province Tumor Hospital | Fu X.,Shenyang University | Song Y.,Shenyang University | Wei M.,Shenyang University | Jin W.,Shenyang University
Chinese Journal of Clinical Oncology | Year: 2010

Objective: To study the effect of docetaxel (DOC) combined with 4-AP on human breast cancer MCF-7 cells and to explore whether 4-AP could strengthen the effect of docetaxel. Methods: MTT assays were performed to investigate the effect of docetaxel, 4-AP and the combination of them on the proliferation of MCF-7 cells. Flow cytometry was employed to detect cell cycles and cell apoptosis after the cells were stained by Pl alone or by Annexin-V and Pl. Results: Docetaxel could significantly inhibit the proliferation of MCF-7 cells in a dose- and time- dependent manner. 4-AP could inhibit the proliferation of MCF-7 cells and the inhibitory rates were 11.9%±1.7%, 42.1%±3.2%, and 44.2±1.6% at 24h, 48h and 72h after adding 4-AP. Moreover 4-AP (5mmol/L) could strengthen the effect of docetaxel. 4-AP (25μmol/L) could increase the effect of Docetaxel. Docetaxel at 5 μ mol/L could significantly increase the percentage of cells at G 2/ M (53.58%±1.44% vs. 8.83%±0.44%, P<0.01) and decrease the percentage of cells at G 0/G 1 (11.48%±0.14% vs. 63.89%± 0.98%, P<0.01), indicating that docetaxel blocked MCF-7 cells at G 2/M phase. 4-AP at 5mmol/L could increase the percentage of MCF-7 cells at G 0/G 1 and decrease the percentage of cells at G 2/M (0.42%±0.17% vs. 8.83%±0.44%, P<0.05). Docetaxel could significantly increase late apoptosis and death of MCF-7 cells after treatment over 24h (from 6.97%±0.75% to 20.77%±0.75%, P<0.05). Docetaxel combined with 4-AP could increase early apoptosis'rate from 4.60%±0.91% to 12.20%±0.82% (P<0.05) and could increase late apoptosis rate and death rate from 4.60%±0.91% to 12.20%±0.82% (P<0.05). Conclusion: Both docetaxel and 4-AP can inhibit the proliferation of MCF-7 cells. Docetaxel can increase the percentage of cells at G 2/M phase and 4-AP can increase the percentage of cells at G 0/G 1 phase. 4-AP could strengthen the inhibitory effect of docetaxel on the proliferation of MCF-7 cells through inducing cell apoptosis. Source

Pang K.,Xuzhou Central Hospital | Pang K.,Shanghai JiaoTong University | Liu S.-B.,Liaoning Province Tumor Hospital | Wei H.-B.,Shanghai JiaoTong University | And 4 more authors.
Lasers in Medical Science | Year: 2014

The thulium laser (Tm-laser) technique has been used in the management of many urologic conditions. The present study aimed to evaluate the use of this technique for distal ureter and bladder cuff (DUBC) excision during nephroureterectomy for upper urinary tract urothelial carcinoma (UUT-UC). Fifty-eight patients with UUT-UC who underwent radical nephroureterectomy were included in this retrospective study. DUBC was managed by open excision in 24 cases, by transurethral electrosurgery in 17 cases, and by transurethral Tm-laser in 17 cases. Perioperative measures and oncologic outcomes were compared among the three groups. Furthermore, 11 human ureteral segments were collected to measure the burst pressure and show physical pressure tolerance, and six ureteral segments were assessed histologically to investigate the sealing effect. Operative time and hospital stay were significantly longer, and intraoperative blood loss was significantly greater in the open excision group than in the electrosurgery and Tm-laser groups (P<0.05 for all). There were no significant differences in these parameters between the electrosurgery and Tm-laser groups. In addition, there were no significant differences in the incidences of bladder tumors and retroperitoneal recurrence of urothelial carcinoma among the three groups. The coagulation time and resection time were significantly shorter in the Tm-laser group than in the electrosurgery group. The mean burst pressure did not differ significantly between the tissues sealed by electrosurgery and by Tm-laser. Histopathological analyses showed that distal ureters were completely sealed by both electrosurgery and Tm-laser. The Tm-laser technique is superior to open excision and comparable to transurethral electrosurgery in the management of DUBC during nephroureterectomy for UUT-UC, offering an alternative treatment option for this condition. © 2013 Springer-Verlag. Source

Hu B.,Liaoning Province Tumor Hospital | Yang H.,Liaoning Province Tumor Hospital | Yang H.,General Hospital of Shenyang Military Command
Tumor Biology | Year: 2014

The aim of this study is to explore the diagnostic role of urine prostate cancer antigen 3 (PCA3) in detecting prostate cancer (PCa) through a systematic review and meta-analysis. Relevant research studies aiming at the application of urine PCA3 level in PCa diagnosis were searched in PubMed, Embase, Chinese Biomedical Database (CBM), Chinese National Knowledge Infrastructure (CNKI), VIP, and Wan Fang databases independently, which were published up to May 8, 2014. The pooled sensitivity, specificity, positive diagnostic likelihood ratio (DLR+), negative diagnostic likelihood ratio (DLR−), diagnostic odds ratio, and the area under the summary receiver operating characteristic were used to evaluate the value of urine PCA3 in diagnosis of PCa by using the Meta-DiSc and STATA 12.0 statistical software. Sixteen research studies with a total 2,457 PCa patients and 4,236 control individuals were included in this meta-analysis. Overall, the results showed sensitivity and specificity of urine PCA3 in the diagnosis of PCa was 0.57 (95 % CI = 0.55–0.59), and 0.71 (95 % CI = 0.70–0.73), respectively. The DLR + and PLR − in the diagnosis of PCa were 2.12 (95 % CI = 1.89–2.38), and 0.55 (95 % CI = 0. 50–0.61), respectively. The pooled diagnostic odds ratio was 3.93 (95 % CI = 3.28–4.72). The area under the curve (AUCs) and *Q index estimate were 0.7118 and 0.6623, respectively. Urine PCA3 is a potential biomarker for the diagnosis of PCa. However, further well-designed studies with large samples will be needed to confirm the results got from present meta-analysis. © 2014, International Society of Oncology and BioMarkers (ISOBM). Source

Wang Y.,Liaoning Province Tumor Hospital | Chou W.-P.,Liaoning Province Tumor Hospital | Shen C.-J.,Shenyang Medical College | Wang X.-Y.,Shenyang Medical College | Luo J.,Shenyang University
Journal of Dalian Medical University | Year: 2013

Objective: To discuss the independent prognostic factors influencing long-term survival rate of the patients with colon cancer after radical resection. Methods: Univariate and Cox multivariate regression analysis was performed on 165 patients treated with radical resection from Jan. 1995 to Jan. 2009. Results: The overall recurrence was 16.36%. Univariate analysis showed that lymph node involvement, tumor size, Dukes'stage and histological differentiation were correlated to recurrence of colon cancer after operation; Multivariate analysis showed that lymph node involvement and histological differentiation were significant factors for recurrence rate of colon cancer. Conclusions: Histological defferentiation is the most important prognositc factor for patients with colon cancer after radical resection, whereas lymph node metastasis is an important prognostic factor. Source

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