Liaoning Province Tumor Hospital

Shenyang, China

Liaoning Province Tumor Hospital

Shenyang, China

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Hu B.,Liaoning Province Tumor Hospital | Yang H.,Liaoning Province Tumor Hospital | Yang H.,General Hospital of Shenyang Military Command
Tumor Biology | Year: 2014

The aim of this study is to explore the diagnostic role of urine prostate cancer antigen 3 (PCA3) in detecting prostate cancer (PCa) through a systematic review and meta-analysis. Relevant research studies aiming at the application of urine PCA3 level in PCa diagnosis were searched in PubMed, Embase, Chinese Biomedical Database (CBM), Chinese National Knowledge Infrastructure (CNKI), VIP, and Wan Fang databases independently, which were published up to May 8, 2014. The pooled sensitivity, specificity, positive diagnostic likelihood ratio (DLR+), negative diagnostic likelihood ratio (DLR−), diagnostic odds ratio, and the area under the summary receiver operating characteristic were used to evaluate the value of urine PCA3 in diagnosis of PCa by using the Meta-DiSc and STATA 12.0 statistical software. Sixteen research studies with a total 2,457 PCa patients and 4,236 control individuals were included in this meta-analysis. Overall, the results showed sensitivity and specificity of urine PCA3 in the diagnosis of PCa was 0.57 (95 % CI = 0.55–0.59), and 0.71 (95 % CI = 0.70–0.73), respectively. The DLR + and PLR − in the diagnosis of PCa were 2.12 (95 % CI = 1.89–2.38), and 0.55 (95 % CI = 0. 50–0.61), respectively. The pooled diagnostic odds ratio was 3.93 (95 % CI = 3.28–4.72). The area under the curve (AUCs) and *Q index estimate were 0.7118 and 0.6623, respectively. Urine PCA3 is a potential biomarker for the diagnosis of PCa. However, further well-designed studies with large samples will be needed to confirm the results got from present meta-analysis. © 2014, International Society of Oncology and BioMarkers (ISOBM).


Meng J.,Shenyang University | Lu X.-B.,Shenyang University | Tang Y.-X.,Shenyang University | Sun G.-P.,Shenyang University | And 5 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2013

The aim of the present study was to determine whether allogeneic red blood cell transfusions showed a deleterious effect and what might be preoperative risk factors for blood transfusion in patients with TNM stage II colon cancer. Total 470 patients who fulfilled inclusion criteria were selected for a further 10-year followup study. We found that there were statistical significance between non-transfused and transfused group in mortality (P=0.018), local recurrence (P=0.000) and distant metastasis (P=0.040). Local recurrence and distant metastasis between 1 to 3 units and more than 3 units group did not show any significant differences. There was no difference in survival rate between non-transfused and 1 to 3 units group (log rank =0.031, P=0.860). The difference between different blood transfusion volume in transfused patients was found (78.77% vs 63.83%, P=0.006). Meanwhile, the significant difference of survival rate was existed between non-transfused group and more than 3 units group (84.83% vs 63.83%, P=0.002 ). Univariate analysis showed the following 3 variables to be associated with an increased risk of allogeneic blood transfusions: preoperative CEA level (P<0.05), location of tumor (P<0.01) and diameter of tumor (P<0.01). Multivariate analysis revealed that location of tumor and diameter of tumor are two independent factors for requirement of perioperative transfusions. Therefore, allogeneic transfusion increase the postoperative tumor mortality, local recurrence and distant metastasis in patients with stage II colon cancer. The postoperative tumor mortality, local recurrence and distant metastasis were not associated with the blood transfusion volume. The blood transfusion volume was associated with the survival rate. Location of tumor and diameter of tumor were the independent preoperative risk factors for blood transfusion.


Ren Y.I.,Shenyang University | Ren Y.I.,Liaoning Province Tumor Hospital | Li Y.,Shenyang Medical College | Tian D.,Shenyang University
Oncology Reports | Year: 2012

ATP-binding cassette transporter E1 (ABCE1), also known as RLI (RNase L inhibitor), is a new type of endo-ribonuclease inhibitor, which can specifically bind to RNase L and abolish its effect. ABCE1 binds to eIF2α and eIF5 to form a pre-translation initiation complex, suggesting its crucial role in cell growth, development and certain pathological processes. To probe the role of ABCE1 in the development and progress of human lung adenocarcinoma, we first detected the changes of its mRNA and protein expression in tissues, and found a high expression level of ABCE1 in human lung adenocarcinoma tissues and metastatic lymph nodes, which was also correlated with clinical stages. Moreover, human lung adenocarcinoma A549 cells were infected with lentiviral vectors containing ABCE1-specific shRNA, and resulted in significant inhibition of cell growth. Using microarray assay, a number of differentially expressed genes were found after ABCE1 suppression. Our results demonstrated the potential role of ABCE1 in human lung adenocarcinoma, which may provide some molecular basis for the mechanisms of development and progress of human lung adenocarcinoma, and help to find new pharmacological targets.


Zhu X.,Shenyang University | Zhu X.,Liaoning Province Tumor Hospital | Liu J.,Shenyang University | Xu X.,Shenyang University | And 2 more authors.
International Journal of Oncology | Year: 2015

The Pleckstrin and Sec7 domain-containing (PSD) gene has been recently found to participate in the progression of several types of cancer. In the present study, we identified PSD as a candidate tumor suppressor gene silenced through epigenetic modification in gastric cancer (GC). PSD mRNA expression and DNA methylation were evaluated by real-time reverse-transcriptase polymerase chain reaction (RT-PCR) and methylation-specific PCR in GC cell lines and tissue samples. Involvement of histone modification in GC cell lines was examined by chromatin immunoprecipitation assay. We also used an siRNA-mediated approach to knock down the PSD gene in SGC7901 cells, which was utilized to detect the role of PSD in GC progression, followed by analysis of cell apoptosis and invasion. PSD gene expression was reduced in all GC cell lines compared with GES1 (an immortalized normal gastric cell line). In addition, PSD expression was markedly downregulated in gastric carcinoma tissues when compared to adjacent non-tumor tissues. Our data also indicated that PSD mRNA and protein levels were associated with tumor differentiation and lymph node metastasis. Aberrant DNA methylation status and histone modification were also found in GC cell lines. Enhanced gene expression was detected when the HGC27, AGS and BGC823 GC cell lines were treated with the DNA-demethylating agent 5-aza-2'-deoxycytidine. However, treatment with trichostatin A, a histone deacetylase inhibitor, had no effect on PSD expression in any of the GC cell lines. Suppression of PSD by siRNA led to enhanced SGC7901 cell invasion. The depletion of PSD expression inhibited cell proliferation and decreased apoptosis in SGC7901 cell lines. Knockdown of the PSD expression decreased caspase-3 and -7 protein levels in SGC7901 cells. PSD gene may function as a tumor suppressor in GC suggesting a vital role for DNA methylation and histone modification in PSD silencing. PSD expression might be a useful biomarker for epigenetic-based GC early diagnosis and may lead to the identification of new targets for pharmacological intervention.


Xu F.,Shenyang University | Dai C.,Shenyang University | Zhang R.,Liaoning Province Tumor Hospital | Zhao Y.,Shenyang University | And 2 more authors.
Digestive Diseases and Sciences | Year: 2012

Background: At present, the relationship between Nanog expression and the biological behavior and prognosis of colorectal cancer is still unclear. Aim: The purpose of this study was to evaluate the expression and regulatory effects of Nanog in colorectal cancer and the correlation between Nanog protein expression and the prognosis of patients with colorectal cancer. Materials and Methods: The differential expression of genes between CD133+ tumor cells and CD133- tumor cells were detected using RT 2 Profiler™ PCR Array. The Nanog mRNA expression level was detected by RT-PCR and the protein level was detected using immunohistochemistry staining. The relationship between Nanog expression and clinicopathological parameters of colorectal cancer was determined. Results: Nanog were expressed significantly higher in CD133+ tumor cells compared to CD133- tumor cells. It was observed that 72 (20.00 %) of the 360 cases positively expressed Nanog. Univariate analyses indicated that Nanog expression was related to histological grade, lymph node metastasis, TNM stage, and liver metastasis (P = 0.005, 0.001, 0.001 and 0.012, respectively). Spearman correlation analysis showed that Nanog expression has a linear correlation to liver metastasis (P = 0.001). After conducting multivariate analysis, histological grade, TNM stage, and Nanog were found to be related to liver metastasis (P = 0.020, 0.01 and 0.001, respectively). In the Cox regression test, the histological grade, Lymph node metastasis, TNM stage, liver metastasis, and Nanog were detected as the independent prognostic factors (P = 0.02, 0.045, 0.01, 0.001 and 0.001, respectively). Conclusions: Nanog protein may be a potential biomarker for postoperative liver metastasis of colorectal cancer. © 2012 Springer Science+Business Media, LLC.


Sun T.,Liaoning Province Tumor Hospital | Fu X.,Shenyang University | Song Y.,Shenyang University | Wei M.,Shenyang University | Jin W.,Shenyang University
Chinese Journal of Clinical Oncology | Year: 2010

Objective: To study the effect of docetaxel (DOC) combined with 4-AP on human breast cancer MCF-7 cells and to explore whether 4-AP could strengthen the effect of docetaxel. Methods: MTT assays were performed to investigate the effect of docetaxel, 4-AP and the combination of them on the proliferation of MCF-7 cells. Flow cytometry was employed to detect cell cycles and cell apoptosis after the cells were stained by Pl alone or by Annexin-V and Pl. Results: Docetaxel could significantly inhibit the proliferation of MCF-7 cells in a dose- and time- dependent manner. 4-AP could inhibit the proliferation of MCF-7 cells and the inhibitory rates were 11.9%±1.7%, 42.1%±3.2%, and 44.2±1.6% at 24h, 48h and 72h after adding 4-AP. Moreover 4-AP (5mmol/L) could strengthen the effect of docetaxel. 4-AP (25μmol/L) could increase the effect of Docetaxel. Docetaxel at 5 μ mol/L could significantly increase the percentage of cells at G 2/ M (53.58%±1.44% vs. 8.83%±0.44%, P<0.01) and decrease the percentage of cells at G 0/G 1 (11.48%±0.14% vs. 63.89%± 0.98%, P<0.01), indicating that docetaxel blocked MCF-7 cells at G 2/M phase. 4-AP at 5mmol/L could increase the percentage of MCF-7 cells at G 0/G 1 and decrease the percentage of cells at G 2/M (0.42%±0.17% vs. 8.83%±0.44%, P<0.05). Docetaxel could significantly increase late apoptosis and death of MCF-7 cells after treatment over 24h (from 6.97%±0.75% to 20.77%±0.75%, P<0.05). Docetaxel combined with 4-AP could increase early apoptosis'rate from 4.60%±0.91% to 12.20%±0.82% (P<0.05) and could increase late apoptosis rate and death rate from 4.60%±0.91% to 12.20%±0.82% (P<0.05). Conclusion: Both docetaxel and 4-AP can inhibit the proliferation of MCF-7 cells. Docetaxel can increase the percentage of cells at G 2/M phase and 4-AP can increase the percentage of cells at G 0/G 1 phase. 4-AP could strengthen the inhibitory effect of docetaxel on the proliferation of MCF-7 cells through inducing cell apoptosis.


Sun P.,China Medical University at Heping | Sun P.,Liaoning Province Tumor Hospital | Liu Y.,China Medical University at Heping | Ying H.,China Medical University at Heping | Li S.,China Medical University at Heping
Oncology Reports | Year: 2012

Medulloblastoma (MB) is one of the most common malignant brain tumors of childhood and is associated with a poor prognosis. Gap-junctional intercellular communication (GJIC) is an important mode for cell-to-cell communication. Dysfunctional GJIC is exhibited in most cancer cells. There is significant evidence that GJIC is important in at least some prodrug/suicide gene systems by augmenting the bystander effect (BE). GJIC is made up of connexins (Cxs), among which Cx43 is present in most tissues. Bcl-2, an important apoptosis blocker, is closely associated with the sensitivity to anticancer drugs. Our study showed that dibutyryl cyclic adenosine monophosphate (db-cAMP) upregulated the Cx43 expression and GJIC function in Daoy medulloblastoma cells. It directly enhanced the BE using a herpes simplex virus thymidine kinase (HSV-tk)/ganciclovir (GCV) system, which was blocked by a Cx43 inhibitor. In addition, db-cAMP increased the cytotoxicity of temozolomide and teniposide, possibly by downregulating the Bcl-2 expression and inducing apoptosis. Taken together, we demonstrated the beneficial effect of db-cAMP in treating medulloblastoma depending on the upregulation of BE and chemosensitivity through Cx43 and Bcl-2-mediated pathways.


Su X.,Liaoning Province Tumor Hospital | You Z.,202 Hospital of PLA | Zheng Z.,Liaoning Province Tumor Hospital
Chinese Journal of Clinical Oncology | Year: 2011

Objective: To investigate and discuss the relationship between the expressions of E-cadherin and S100A2 genes in gastric cancer tissues, as well as their clinical characters. Methods: Semi-quantitative RT-PCR and immunohistochemistry were performed in the tumor tissues with various grades, paracancerous, and normal gastric tissues to observe the functions of E-cadherin and S100A2 in gastric cancer tissues. Sixty-four cases of tumor tissues with various grades were collected from July 2006 to December 2006 in the Liaoning Province Tumor Hospital, and 15 gastric para-neoplastic and normal gastric tissues were collected as controls. Statistical methods were used to analyze the relationship between the clinical characters of E-cadherin and S100A2 and their roles in tumor progression. Results: The length of the amplified products of E-cadherin and S100A2 was 487 bp and 362 bp, respectively. The positive expression of E-cadherin and S100A2 genes in normal gastric tissues was both 100%, whereas the expression was lower in gastric cancer tissues. Moreover, same results were obtained in the positive expression of E-cadherin and S100A2 proteins. The expression of E-cadherin and S100A2 had high consistency in gastric cancer tissues. The positive expression of E-cadherin and S100A2 proteins was statistically significant in clinical characters, such as gross type, differentiation, invasion depth, and lymph node metastasis. Conclusion: E-cadherin and S100A2 are tumor-inhibiting factors, and their expression in gastric cancer tissues decreased. The expression was negatively correlated with to tumor differentiation grade, lymph node metastasis, and invasion grade.


Zhu X.,Liaoning Medical University | Zhu X.,Liaoning Province Tumor Hospital | Liu J.,Liaoning Medical University | Xu X.,Liaoning Medical University | And 2 more authors.
Oncology Reports | Year: 2015

The present study aimed to identify novel histone modification markers in gastric cancer (GC) by chromatin immunoprecipitation microarray (ChIP-chip) analysis and to determine whether these markers were able to discriminate between normal and GC cells. We also tested for correlations with DNA methylat ion. We prob e d a hu man CpG isla nd m icro - array with DNA from a GC cell line (MKN45) by chromatin immunoprecipitation (ChIP). ChIP-reverse-transcriptase quantitative polymerase chain reaction PCR (RT-qPCR) was used to validate the microarray results. Additionally, mRNA expression levels and the DNA methylation of potential target genes were evaluated by RT-qPCR and methylation-specific PCR (MSP). The moults showed that 134 genes exhibited the highest signal-to-noise ratio of H3-K 9 trimethylation overacetylation and 46 genes exhibited the highest signal-to-noise ratio of H3-K9 trimethylation over H3-K4 trimethylation in MKN45 cells. The ChIP-qPCR results agreed with those obtained from the ChIP-chip analysis. Aberrant DNA methylation status and mRNA expression levels were also identified for selected genes (PSD, SMARCC1 and Vps37A) in the GC cell lines. The results suggest that CpG island microarray coupled with ChIP (ChIP-chip) can identify novel targets of gene silencing in GC. Additionally, ChIP-chip is the best approach for assessing the genome-wide status of epigenetic regulation, which may allow for a broader genomic understanding compared to the knowledge that has been accumulated from single-gene studies.


PubMed | Liaoning Province Tumor Hospital
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2014

The aim of this study is to explore the diagnostic role of urine prostate cancer antigen 3 (PCA3) in detecting prostate cancer (PCa) through a systematic review and meta-analysis. Relevant research studies aiming at the application of urine PCA3 level in PCa diagnosis were searched in PubMed, Embase, Chinese Biomedical Database (CBM), Chinese National Knowledge Infrastructure (CNKI), VIP, and Wan Fang databases independently, which were published up to May 8, 2014. The pooled sensitivity, specificity, positive diagnostic likelihood ratio (DLR+), negative diagnostic likelihood ratio (DLR-), diagnostic odds ratio, and the area under the summary receiver operating characteristic were used to evaluate the value of urine PCA3 in diagnosis of PCa by using the Meta-DiSc and STATA 12.0 statistical software. Sixteen research studies with a total 2,457 PCa patients and 4,236 control individuals were included in this meta-analysis. Overall, the results showed sensitivity and specificity of urine PCA3 in the diagnosis of PCa was 0.57 (95 % CI=0.55-0.59), and 0.71 (95 % CI=0.70-0.73), respectively. The DLR + and PLR - in the diagnosis of PCa were 2.12 (95 % CI=1.89-2.38), and 0.55 (95 % CI=0. 50-0.61), respectively. The pooled diagnostic odds ratio was 3.93 (95 % CI=3.28-4.72). The area under the curve (AUCs) and *Q index estimate were 0.7118 and 0.6623, respectively. Urine PCA3 is a potential biomarker for the diagnosis of PCa. However, further well-designed studies with large samples will be needed to confirm the results got from present meta-analysis.

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