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Feng W.-H.,Liaoning Medical University | Feng W.-H.,Liaoning Province Research Institute of Family Planning | Xin Y.,Liaoning Medical University
Tumor | Year: 2013

CypA (cyclophilin A), the main intracellular receptor of immunosuppressant CsA (cyclosporin A), is the first discovered and most abundant one in cyclophilin family and has been studied in-depth. CypA has PPIase (peptidyl-prolyl cis-trans isomerase) activity and molecular chaperone functions which enable it playing a role in correct protein folding. CypA also involves in a variety of biological functions such as immune suppression, inflammatory response, and oxidative stress response. Recent studies have found that CypA was overexpressed in many malignant tumors, and it can promote the proliferation of tumor cells, inhibit apoptosis, and mediate tumor invasion and metastasis. It may become a new diagnostic and therapeutic target of some malignant tumors in future. Copyright © 2013 by TUMOR.

Li H.-T.,Shenyang University | Li H.-T.,Liaoning Province Research Institute of Family Planning | Jiang F.-X.,University of Western Australia | Shi P.,Shenyang University | And 4 more authors.
Biochemical and Biophysical Research Communications | Year: 2012

Islet cell replacement therapy represents the most promising approach for the cure of type 1 diabetes if autoimmunity to β cells is under control. However, this potential is limited by a shortage of pancreas donors. To address the donor shortage problem, we determined whether bone marrow-derived mesenchymal stem cells (bmMSCs) can be directly reprogrammed to islet lineages by simultaneously forced suppression and over-expression of key regulator genes that play critical roles during pancreas development. Here, we report that rat bmMSCs were converted . in vitro into insulin-producing cells by suppressing two-repressor genes repressor element-1 silencing transcription factor/neuronal restrictive silencing factor (Rest/Nrsf) and sonic hedgehog (Shh) and by over-expressing pancreas and duodenal transcription factor 1 (Pdx1). The reprogrammed bmMSCs expressed both genes and proteins specific for islet cells. These converted cells were capable of releasing insulin in a glucose-responsive manner. Our study suggests that bmMSCs may ultimately be reprogrammed to functional insulin-secreting cells. © 2012.

Liu K.,Liaoning Medical University | Lu Y.,Liaoning Province Research Institute of Family Planning | Ai L.,Liaoning Medical University | Jiao B.,Liaoning Medical University | And 3 more authors.
British Journal of Oral and Maxillofacial Surgery | Year: 2015

Non-syndromic orofacial clefts are among the most common congenital defects, and several reports have shown that the FOXE1 gene has strong associations with them. To find out if the gene was a risk factor we used a case-control and family-based analysis, and recruited 230 patients with non-syndromic oral clefts including 179 with non-syndromic cleft lip with or without cleft palate, and 51 with non-syndromic cleft palate alone, their parents (166 mothers and 161 fathers, including 135 complete trios), and 180 healthy controls. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to genotype the 2 most strongly associated markers (rs4460498 and rs3758249) in FOXE1, and case-control and family-based associations were analysed. In the case-control analyses we found a significant association with non-syndromic cleft lip and palate in rs4460498 (p=0.009) and rs3758249 (p=0.014), but no association in patients with cleft palate alone. For rs4460498 in FOXE1, the odds ratio (OR) for cases with CC homozygotes compared with TC+CC genotypes was 1.813 (95% CI 1.176 to 2.796), and for rs3758249 in FOXE1, the OR for cases with GG homozygotes compared with those with AG+AA genotypes was 0.561 (95%CI 0.371 to 0.848). The results of transmission-disequilibrium tests for rs4460698 and rs3758249 for non-syndromic orofacial clefts were p=0.003, OR=2.781 (95% CI 1.414 to 5.469) and p=0.001, OR=2.552 (95%CI 1.574 to 4.138), respectively. This suggests that FOXE1 (rs4460498 and rs3758249) is strongly associated with non-syndromic cleft lip and palate in populations in northeast China, and further study between FOXE1 and non-syndromic orofacial clefts is necessary. © 2015 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

Lu Y.,Liaoning Province Research Institute of Family Planning | Liu Q.,Shenyang University | Xu W.,Liaoning Province Research Institute of Family Planning | Li Z.,Shenyang University | And 9 more authors.
PLoS ONE | Year: 2013

Nonsyndromic cleft lip with or without cleft palate (NSCL/P) are common birth defects with a complex etiology. Multiple interacting loci and possible environmental factors influence the risk of NSCL/P. 12 single nucleotide polymorphisms (SNPs) in 7 candidate genes were tested using an allele-specific primer extension for case-control and case-parent analyses in northeast China in 236 unrelated patients, 185 mothers and 154 fathers, including 128 complete trios, and 400 control individuals. TGFA and IRF6 genes showed a significant association with NSCL/P. In IRF6, statistical evidence of an association between rs2235371 (p = 0.003), rs2013162 (p<0.0001) and NSCL/P was observed in case-control analyses. Family based association tests (FBATs) showed over-transmission of the C allele at the rs2235371 polymorphism (p = 0.007). In TGFA, associations between rs3771494, rs3771523 (G3822A), rs11466285 (T3851C) and NSCL/P were observed in case-control and FBAT analyses. Associations between other genes (BCL3, TGFB3, MTHFR, PVRL1 and SUMO1) and NSCL/P were not detected. © 2013 Lu et al.

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