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Diao Y.,Shenyang University of Technology | Dang J.,Shenyang University | Liu X.-N.,Shenyang University of Technology | Guan W.-Z.,Shenyang University | And 5 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2012

OBJECTIVE: To investigate the protective effect of MnTBAP on radiation-induced lung injury in the rats model. METHODS: Male SD rats were divided into three groups: control group, radiation injured group, and MnTBAP treated group. lung injury models were made by putting the rats under the exposure of 6 MV X-ray at a dose of 28 Gy thoracically. Then the normal saline was injured intraperitoneally immediately of those rats in the injured group, while, all other things equal, MnTBAP(10 mg/kg) was injected to the treated group. After 1, 4, 8 and 12 weeks, using biochemical methods to test the superoxide dismutase(SOD), malondialdehyde(MDA), anti-superoxide anion radicals(ASAR) activity, hydroxyproline(Hyp) and the serum of transforming growth factor-β1(TGF-β1) level. RESULTS: When comparing these two groups, the pathological test shows that the injury level of MnTBAP group was much slighter than that of injured group. Meanwhile, biochemical test result shows that the content of SOD increased notably after 1 to 12 weeks time (all P<0.001), in addition the ASAR incresed significantly too (all P<0.001). On the contrary, the amount of MDA significantly decreased (1 week, P<0.001; 4-12 weeks, all P<0.05), the amount of TGF-β1 significantly decreased (all P<0.001). Moreover, the Hyp, stayed at the same level for the first 4 weeks but reduced significantly during 8-12 weeks (8 weeks, P<0.05; 12 weeks, P<0.01). CONCLUSIONS: MnTBAP has the function of antioxidant and the function of eliminating the free radical. It also has the protecting function to radiation-induced lung injury. In this case, it may be a potential radio-protector.

Gao Y.,Tieling Central Hospital | Zhao H.,Liaoning Province Peoples Hospital | Lu Y.,Fushun Central Hospital | Li H.,Qinghe District Hospital of Tieling City | Yan G.,Dandong Central Hospital
Tumor Biology | Year: 2014

Emerging evidence has suggested that microRNAs (miRNAs) may be promising novel biomarkers for the diagnosis of renal cell carcinoma (RCC). However, the results of current studies are still conflicting. Hence, we undertake the current meta-analysis to comprehensively assess the diagnostic potential of miRNAs in RCC. The bivariate meta-analysis model was employed to summarize the sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR). Summary receiver operating characteristic (SROC) curve and area under the curve (AUC) were used to evaluate the diagnostic accuracy. Subgroup analyses and meta-regression were used to explore the between-study heterogeneity. Deeks’ funnel plot asymmetry test was used to test the potential of publication bias. All analyses were performed using STATA software (version 12.0). The pooled sensitivity and specificity of miRNAs for the diagnosis of RCC were 0.85 (95 % confidence interval (CI), 0.77–0.90) and 0.84 (95 % CI, 0.70–0.92). The value of AUC was 0.91 (95 % CI, 0.88–0.93), suggesting that the diagnostic accuracy of miRNAs achieved a relatively high level. Furthermore, subgroup analyses showed that tissue-based miRNA assay is recommended to improve the diagnostic accuracy. In conclusion, the high degree of diagnostic accuracy suggests that miRNA in RCC patients may serve as next-generation biomarkers for detection of the disease. However, large-scale investigations and additional improvements are urgently needed to confirm our results and verify the feasibility of routine clinical utilization. © 2014, International Society of Oncology and BioMarkers (ISOBM).

Han M.,Jiamusi Central Hospital | Zhang J.S.,Liaoning Medical University | Zhang R.J.,Liaoning Province Peoples Hospital
International Journal of Ophthalmology | Year: 2010

Aim; To investigate the change of p21 ,CDK2 and Smad4 on the HLEC transfected with pEGFP-TGF-pl plasmid and the effect of TGF-f31gene transfection on the cell cycle control of human lens epithelial cell. Methods; Immortalized human lens epithelial cellline (HLEC- B3) was transfered by pEGFP-TGF-pl gene. The expression of TGF-plmRNA, p21mRNA, CDK2mRNA and Smad4 mRNA and protein induced by transfered by TGF- βl gene were measured by RT-PCR and Western- blot at different time periods . RESULTS; RT-PCR detected that TGF-plmRNA began to raise after 24h, culminated after 48h .decreased after 72h, decreased much after 96h,but still more than the normal. p21 mRNA companied with it. but CDK2 decreased with it. Western-blot detected that the consequence consisted with RT-PCR. The effect of TGF-pl gene was demonstrated from the lever of transcription and translation. Smad4 protein raised with the lever of TGF-pl gene at various time point, but disappeared very quickly. Conclusion: TGF-βl can increase p21, but decrease CDK2,to impact the cell cycle of HLEC. TGF-p/Smad4 pathway take part in the course.

Li F.-S.,Liaoning Province Peoples Hospital | Xu S.-N.,Liaoning Province Peoples Hospital | Huang H.,Liaoning Province Peoples Hospital | Du Z.-G.,Liaoning Province Peoples Hospital | Wang L.,Liaoning Province Peoples Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Osteosarcoma combined with pathological fracture was often treated by amputation. With clinical application of neoadjuvant chemotherapy and improvement of technology and material of the prosthesis, the limbs of patients with osteosarcoma combined with pathological fracture were reserved. OBJECTIVE: To evaluate the effect of the application of custom-manufactured artificial total knee prosthesis replacement in limb salvage treatment for patients with osteosarcoma combined with pathological fracture. METHODS: A total of 11 patients with pathological fracture with osteosarcoma located in adjacent knee Joint were enrolled from June 2002 to September 2012. In accordance with individual condition of the patient, limb salvage treatment was designed for knee prosthesis for a tumor. Follow-up results were retrospectively analyzed. There were six males (54.5%) and five females (45.5%) and their age ranged from 11 to 50 years old with an average age of 25.4 years old. Onset regions: seven tumors were located in the distal femur and four tumors were in the proximal tibia. All patients received neoadjuvant chemotherapy, and limb salvage treatment with custom-manufactured artificial knee prosthesis. The follow up was performed for 9 to 105 months. RESULTS AND CONCLUSION: In 11 patients after prosthesis replacement, the regional recurrence rates were 18%, lung metastasis rates were 36%. The five-year survival rate was 58%. Four patients developed lung metastases and died in 9 to 24 months postoperatively. During the last follow-up, Enneking score of the affected limbs was 11 to 30 points, averagely 23.3 points, with an excellent and good rate of 82%. Results indicated that combined with neoadjuvanet chemotherapy, the application of custom-manufactured artificial total knee prosthesis replacement in the treatment of osteosarcoma located in adjacent knee joint and pathological fracture achieved an ideal outcomes of the limb salvage treatment in the near future. The lone-term effectiveness was expected to be evaluated.

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