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Yang X.,Xi'an Jiaotong University | Li Y.,Xi'an Jiaotong University | He Y.,Xi'an Jiaotong University | Li T.,Xi'an Jiaotong University | And 5 more authors.
International Immunopharmacology | Year: 2015

Cordycepin (Cor), which is a naturally occurring nucleoside derivative isolated from Cordyceps militaris, has been shown to exert excellent antiinflammatory activity in a murine model of acute lung injury. Thus, this study aimed to evaluate the antiasthmatic activity of Cor (10, 20, and 40 mg/kg) and to investigate the possible underlying molecular mechanisms. We found that Cor attenuated airway hyperresponsiveness, mucus hypersecretion, and ovalbumin (Ova)-specific immunoglobulin (Ig) E, and alleviated lung inflammation with decreased eosinophils and macrophages in the bronchoalveolar lavage (BAL) fluid. Notably, Cor reduced the upregulation of eotaxin, intercellular cell adhesion molecule-1 (ICAM-1), IL-4, IL-5, and IL-13 in the BAL fluid. Furthermore, Cor markedly blocked p38-MAPK and nuclear factor-kappaB (NF-κB) signalling pathway activation in the Ova-driven asthmatic mice. In conclusion, this study demonstrated that some of the antiasthmatic benefits of Cor attributable to diets and/or tonics may result from reductions in inflammatory processes and that these antiasthmatic properties involve the inhibition of Th2-type responses through the suppression of the p38-MAPK and NF-κB signalling pathways. © 2015 Elsevier B.V. All rights reserved.


Huo M.,Jilin University | Cui X.,Jilin University | Xue J.,Inner Mongolia University of Science and Technology | Chi G.,Inner Mongolia University for Nationalities | And 7 more authors.
Journal of Surgical Research | Year: 2013

Background: Inflammation, characterized by redness, swelling, pain and a sensation of heat, is one of the body's self-defense systems. Although the inflammation response has an important role in host survival, it also leads to chronic inflammatory diseases. Linalool is a natural compound of the essential oils in several aromatic plants species. It possesses anti-inflammatory, antinociceptive, and other bioactive properties. In the present study, we investigated the protective effects of linalool on inflammation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and an LPS-induced in vivo lung injury model. Methods: We evaluated the effects of linalool on LPS-induced production of inflammatory mediators in Raw 264.7 murine macrophages by enzyme-linked immunosorbent assay and Western blot. To confirm the anti-inflammatory activity of linalool in vivo, we induced an acute lung injury in an LPS-induced mouse model. Results: Linalool attenuated the production of LPS-induced tumor necrosis-α and interleukin-6 both in vitro and in vivo. Furthermore, phosphorylation of IκBα protein, p38, c-Jun terminal kinase, and extracellular signal-regulated kinase in LPS-stimulated RAW 264.7 cells was blocked by linalool. Our in vivo study also found that linalool attenuated lung histopathologic changes in mouse models. Conclusions: The results suggest that linalool inhibits inflammation both in vitro and in vivo, and may be a potential therapeutic candidate for the treatment of inflammatory diseases. © 2013 Elsevier Inc. All rights reserved.


Huo M.,Jilin University | Gao R.,Jilin University | Jiang L.,Jilin University | Cui X.,Jilin University | And 7 more authors.
International Immunopharmacology | Year: 2013

Gossypol, a yellowish polyphenolic compound originally from cotton plant, has been known to exert a potential for anti-cancer, anti-inflammatory and other important therapeutic activities. The purpose of this investigation was to determine the protection of gossypol on inflammation in Lipopolysaccharide (LPS) stimulated RAW 264.7 cells and LPS induced in vivo lung injury model. The effects of gossypol on pro-inflammatory cytokines and signaling pathways were evaluated by enzyme-linked immunosorbent assay and Western blot. The results showed that gossypol significantly inhibited the production of LPS-induced TNF-α, IL-6 and IL-1β both in vitro and vivo. Furthermore, gossypol blocked the phosphorylation of IκBα protein, p65, p38, c-Junterminal kinase (JNK) and extracellular signal-regulated kinase (ERK) in LPS stimulated RAW 264.7 cells. From the in vivo study, it was observed that gossypol attenuated lung histopathologic changes in mouse models. The present data suggest that gossypol suppresses the inflammation in vitro and vivo, and may be a potential therapeutic candidate for the treatment of inflammatory disorders. © 2013 Elsevier B.V.


Deng Y.,Jilin University | Guan M.,Jilin University | Xie X.,Jilin University | Yang X.,Jilin University | And 6 more authors.
International Immunopharmacology | Year: 2013

Our group recently reported the strong anti-inflammatory effects of geniposide (Gen), a bioactive iridoid glucoside derived from gardenia jasminoides, in a mouse acute lung injury model. Herein, we hypothesized that Gen might also have potential therapeutic benefits in treatment of asthma, which was tested in a mouse model of ovalbumin (Ova)-induced allergic airway inflammation. Ova-sensitized and -challenged BALB/c mice, as compared with control animals, displayed airway hyperresponsiveness (AHR), bronchoalveolar lavage eosinophilia, mucus hypersecretion, and increased T help 2 (Th2)-associated cytokine and chemokine amounts, as well as serum Ova-specific immunoglobulin E (IgE) level. Being compared with the Ova-induced hallmarks of asthma, intraperitoneal Gen treatment prevented eosinophilic pulmonary infiltration, attenuated the increases in interleukin (IL)-4, IL-5, and IL-13, and reduced eotaxin and vascular cell adhesion molecule 1 (VCAM-1) expression. Also, Gen significantly ameliorated the Ova-driven airway hyperresponsiveness, mucus hypersecretion, and allergen-specific IgE level, which are the cardinal pathophysiological symptoms in allergic airway diseases. In addition, the efficacy of Gen was comparable to that of dexamethasone (Dex), a currently available anti-asthmatic drug. Collectively, our findings reveal that the development of immunoregulatory strategies based on Gen may be considered as an effective adjuvant therapy for allergic asthma. © 2013 The Authors.


PubMed | Jilin University, Xi'an Jiaotong University and Liaoning Province Academy of Analytic Science
Type: Journal Article | Journal: International immunopharmacology | Year: 2015

Cordycepin (Cor), which is a naturally occurring nucleoside derivative isolated from Cordyceps militaris, has been shown to exert excellent antiinflammatory activity in a murine model of acute lung injury. Thus, this study aimed to evaluate the antiasthmatic activity of Cor (10, 20, and 40 mg/kg) and to investigate the possible underlying molecular mechanisms. We found that Cor attenuated airway hyperresponsiveness, mucus hypersecretion, and ovalbumin (Ova)-specific immunoglobulin (Ig) E, and alleviated lung inflammation with decreased eosinophils and macrophages in the bronchoalveolar lavage (BAL) fluid. Notably, Cor reduced the upregulation of eotaxin, intercellular cell adhesion molecule-1 (ICAM-1), IL-4, IL-5, and IL-13 in the BAL fluid. Furthermore, Cor markedly blocked p38-MAPK and nuclear factor-kappaB (NF-B) signalling pathway activation in the Ova-driven asthmatic mice. In conclusion, this study demonstrated that some of the antiasthmatic benefits of Cor attributable to diets and/or tonics may result from reductions in inflammatory processes and that these antiasthmatic properties involve the inhibition of Th2-type responses through the suppression of the p38-MAPK and NF-B signalling pathways.


Xue Z.,Dalian Ocean University | Li H.,Dalian Ocean University | Wang X.,Dalian Ocean University | Li X.,Dalian Ocean University | And 3 more authors.
Fish and Shellfish Immunology | Year: 2015

It is very important to identify and characterize the immune-related genes that respond to pathogens. Until recently, only some of the immune-related genes in sea cucumbers had been characterized. Their expression patterns after pathogen challenges have been analyzed via expressed sequence tag libraries, microarray studies and proteomic approaches. These genes include lectins, antimicrobial peptides, lysozyme, enzymes, clotting protein, pattern recognition proteins, Toll receptors, complement C3 and other humoral factors that might participate in the innate immune system of sea cucumbers. Although the participation of some of these immune molecules in the sea cucumber's innate immune defense against invading pathogens has been demonstrated, the functions of many of the molecules remain unclear. This review focuses on the discovery and functional characterization of the immune-related molecules from the sea cucumber for the first time and provides new insights into the immune mechanisms of the sea cucumber, which opens new possibilities for developing drugs for novel anti-bacterial and antiviral applications in fisheries. © 2015 Elsevier Ltd.


Dai R.,Shenyang Pharmaceutical University | Ren X.,Liaoning Province Academy of Analytic Science | He X.,Shenyang Pharmaceutical University | Huo Y.,Shenyang Pharmaceutical University
Bulletin of Environmental Contamination and Toxicology | Year: 2011

A convenient analytical method for quantitative characterization of 23 pesticides in three herbs has been developed. Pesticides tested included organochlorine, organophosphorus and pyrethroids. Primary secondary amine and graphitized carbon black as dispersive-SPE sorbent were applied to clean up the sample. Analytical method was established by using the technique of gas chromatography coupled with electron impact mass spectrometry in the selected ion monitoring mode (GC-MS-SIM). The recoveries of all pesticides were in the range of 78.4%-119.2% at three spiked levels of 5, 20 and 50 μg/kg, and the relative standard deviations were below 9.5%. The limits of detections of all pesticides were less than 3.0 μg/kg. This analytical method could be applied to the analysis of commonly used pesticides in commercial herbs. © 2011 Springer Science+Business Media, LLC.


Mi C.,Northeastern University China | Tian Z.,Northeastern University China | Cao C.,Liaoning Province Academy of Analytic science | Wang Z.,Liaoning Province Academy of Analytic science | And 2 more authors.
Langmuir | Year: 2011

This work reports the novel microwave-assisted solvothermal synthesis and structural, topographic, spectroscopic characterization of NaYF 4:Yb,Er upconversion nanoparticles (UCNPs) as well as their application in the labeling of HeLa cells. The nanoparticles were prepared in ethylene glycol, with rare earth acetates as precursor and NH 4F and NaCl as the fluorine and sodium sources. X-ray diffraction, transmission electron microscopy, and luminescence spectroscopy were applied to characterize the nanoparticles. Experimental results showed that the microwave-assisted solvothermal method is an effective approach to create highly crystalline, strongly luminescent UCNPs at a lower temperature (160 °C) and within a significantly shortened reaction time (only 1 h) compared to the traditional methods. The effect of fluorine source on the optical properties of UCNPs was investigated by using NH 4F, NH 4HF 2, NaF, and 1-butyl-3-methylimidazolium tetrafluoroborate (BmimBF 4) as different fluorine sources; NH 4F proved to be the best one, making the luminescent intensity increase at least 2 orders of magnitude. The UCNPs with four different colors (green, yellow, orange, and cyan) were successfully obtained. After being modified with amino groups and coupled with CEA-8 antibody, the obtained nanoparticles were successfully applied in the specific fluorescent immunolabeling and imaging of HeLa cells to further verify their function as a marker in immunolabeling. © 2011 American Chemical Society.


Li H.,Liaoning Province Academy of Analytic science | Fulin T.,Liaoning Province Academy of Analytic science | Ren X.,Liaoning Province Academy of Analytic science | Wang X.,Liaoning Province Academy of Analytic science
Chinese Journal of Chromatography (Se Pu) | Year: 2011

A method based on gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed for the determination of phthalic acid esters (PAEs) in soil. The soil sample was extracted by dichloromethane-acetone (l:1, v/v) with an ultrasonic instrument; and the extract was cleaned up with a Florisil column. The identification and quantification of the analytes were carried out in the multiple reaction monitoring (MRM) mode after the GC separation on an HP-5MS column (30 m ×0. 25 mm ×0. 25 μm). The eight point calibration curves showed a good linearity for all the congeners (0. 997 3 - 0. 997 6). The limits of detection (LODs) ranged from 0. 1 to 0. 5 μg/kg. The relative standard deviations (RSDs) were not more than 14. 3% and the spiked recoveries were in the range of 72. 9% - 106. 2%. The developed analytical method is rapid and accurate, and suitable for the determination of PAEs in soil.


PubMed | Dalian Institute of Product Quality Supervision & Inspection, Dalian Ocean University and Liaoning Province Academy of Analytic Science
Type: Journal Article | Journal: Fish & shellfish immunology | Year: 2015

It is very important to identify and characterize the immune-related genes that respond to pathogens. Until recently, only some of the immune-related genes in sea cucumbers had been characterized. Their expression patterns after pathogen challenges have been analyzed via expressed sequence tag libraries, microarray studies and proteomic approaches. These genes include lectins, antimicrobial peptides, lysozyme, enzymes, clotting protein, pattern recognition proteins, Toll receptors, complement C3 and other humoral factors that might participate in the innate immune system of sea cucumbers. Although the participation of some of these immune molecules in the sea cucumbers innate immune defense against invading pathogens has been demonstrated, the functions of many of the molecules remain unclear. This review focuses on the discovery and functional characterization of the immune-related molecules from the sea cucumber for the first time and provides new insights into the immune mechanisms of the sea cucumber, which opens new possibilities for developing drugs for novel anti-bacterial and antiviral applications in fisheries.

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