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Tian A.-X.,Bohai University | Lin X.-L.,Bohai University | Liu Y.-J.,Bohai University | Liu G.-Y.,Liaoning Ocean and Fisheries Science Research Institute | And 3 more authors.
Journal of Coordination Chemistry | Year: 2012

Through tuning the oxidation states of Cu I/II, two Keggin-based compounds with different topologies, [Cu I 5(btx) 4(PW VI 10W V 2O 40)] (1) and [Cu 2 II(btx) 4(SiW 12O 40)] (2) (btx = 1,6-bis(1,2, 4-triazol-1-y1)hexane), were synthesized and structurally characterized. In 1, there exist decanuclear Cu I circuits, which are linked through N1-containing btx ligands to construct a grid-like 2-D layer. Three sets of these layers interpenetrate to build a three-fold interpenetrating framework. The Keggin polyanions connect the adjacent three-fold interpenetrating frameworks to construct a 3-D structure. In 2, the Cu II-btx moiety shows a (6 6) 3-D hexagonal channel-style framework. The Keggin polyanion offering four terminal oxygen atoms incorporates with this metal-organic framework imbedding the channels. The different oxidation states of copper ions (+I/+II) induces distinct coordination modes of btx and Keggin polyanions and affects the whole structural topologies. Tuning the oxidation states of copper ions is an effective strategy for obtaining POM-based topologies. In addition, the electrochemical properties of 1 and 2 bulk-modified carbon-paste electrodes are reported. © 2012 Taylor & Francis. Source


Mi X.,Northwest University, China | Wei Z.,Dezhou University | Zhou Z.,Liaoning Ocean and Fisheries Science Research Institute | Liu X.,Northwest University, China
Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics | Year: 2014

MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs that regulate gene expression by post-transcriptional repression of messenger RNA. The echinoderm, Strongylocentrotus nudus, is an excellent model organism for studying development and commercially important as a food source. However, to date no miRNAs have been reported to modulate sex gonad differentiation in S. nudus. In this study, we constructed two small RNA libraries from male and female S. nudus gonad respectively for Solexa sequencing. A total of 184 miRNAs including 60 known and 124 novel miRNAs were identified from the two libraries. Furthermore, the nucleotide bias and end variation of the known miRNAs were also analyzed. In addition, 67 differently expressed of the 86 co-expressed and 98 gender-specific (47 male-specific and 51 female-specific) miRNAs that may be involved in sexual differentiation were found by comparing the miRNA expression profiles in the two libraries. This study reveals the first miRNA profile related to the gonad differentiation of the S. nudus. This study gives a first insight into sex differences in miRNA expression of sea urchin which could facilitate studies of the reproductive organ-specific roles of miRNAs. © 2014 Elsevier Inc. Source


Yang J.,Jiangsu University | Yang J.,Dalian Polytechnic University | Zhu B.,Jiangsu University | Zhu B.,Dalian Polytechnic University | And 5 more authors.
Carbohydrate Polymers | Year: 2013

In this study, glycogen was extracted from oyster Ostrea talienwhanensis Crosse and used as a model to investigate the structure-Activity correlation of polysaccharides. Purified oyster glycogen was characterized by methylation analysis, nuclear magnetic resonance (NMR) spectroscopy and infrared spectroscopy (IR). The oyster glycogen was subsequently sulfated by chlorosulfonic acid-pyridine method, and a C-6 substituted species (SOG) was identified to be the primary sulfated oyster glycogen species by 13C NMR spectroscopy. The molecular weight and sulfate content of the SOG was determined to be 3.2 × 104 g/mol and 33.6%, respectively. Another sulfated oyster glycogen species (SOG1) with C-2 and C-3 substitution was also identified at a lesser amount in the final product. SOG exhibited a much stronger stimulation effect to splenic lymphocyte proliferation than SOG1 in vitro, indicating that the position of sulfate substitution is a major determining factor on the efficacy of sulfated glycogens to stimulate lymphocyte proliferation. © 2013 Elsevier Ltd. All rights reserved. Source


Wang K.,Liaoning Ocean and Fisheries Science Research Institute | Wang N.-B.,Liaoning Ocean and Fisheries Science Research Institute
Shuidonglixue Yanjiu yu Jinzhan/Chinese Journal of Hydrodynamics Ser. A | Year: 2010

Based on natural situation in Liaodong bay, the numerical diffusion model which was used to investigate the impact of pollutant point source input was proposed to analysis the distributions of the nutrient concentrations in Liaodong bay. The distributions of the nutrient elements were studied in detail. The numerical simulation results compared with actual observed data proved the accuracy and practicability of the method. Source


Li H.,National Marine Environmental Monitoring Center | Liu S.,National Marine Environmental Monitoring Center | He C.,Liaoning Ocean and Fisheries Science Research Institute | Gao X.,Liaoning Ocean and Fisheries Science Research Institute | Yuan X.,National Marine Environmental Monitoring Center
Aquatic Biology | Year: 2013

Heat shock proteins (HSPs) are molecular chaperones that help organisms cope with stressful conditions. In this study, a novel small HSP (sHSP) gene was identified from the hard clam Meretrix meretrix (designated as Mm-HSP20). The full length of the cDNA is 1222 bp, consisting of a 5'-terminal untranslated region (5'UTR) of 103 bp, a 3'UTR of 565 bp, and a 522 bp open reading frame encoding a polypeptide of 173 amino acids. Sequence comparison showed that Mm-HSP20 had a moderate degree of homology to the sHSP of other organisms. A sHSP feature domain, an alpha-crystallin domain, and a V/IXI/V motif in the C-terminal extension were identified in the Mm-HSP20 amino acid sequence, indicating that Mm-HSP20 is a new member of the sHSP family. The Mm-HSP20 transcript was constitutively expressed in 6 different test tissues, with the highest expression level detected in the digestive gland by fluorescent quantitative realtime PCR. The temporal expression of Mm-HSP20 mRNA in the digestive gland and hemocytes was evaluated after exposure to cadmium (Cd) (40 μg l-1CdCl2) and benzo[a]pyrene (50 μg l-1B[a]P) individually, and in combination (40 μg l-1 CdCl2 +5 0 μg l-1 B[a]P). Mm-HSP20 expression level increased significantly after Cd and B[a]P exposure in both tissue types. Conversely, significant Mm-HSP20 transcript repression was detected in the 24 h B[a]P-treated digestive gland samples. Considerable up-regulation of Mm-HSP20 mRNA level was observed after multiple exposures. These results indicate that Mm-HSP20 plays a role in mechanisms involved in coping with environmental stress in hard clams. Mm-HSP20 may be suitable for use as a biomarker for heavy metal and B[a]P contamination. © Inter-Research 2013. Source

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