Liaoning Medical College
Liaoning Medical College
Sui R.,First Affiliated Hospital of Liaoning Medical College |
Zhang L.,Liaoning Medical College
Neural Regeneration Research | Year: 2011
Electroacupuncture was performed at the Wangu (GB 12) acupoint, whose position is similar to the cerebellar fastigial nucleus in rats with post-stroke depression. Results showed that the expression of nuclear factor-κB and the levels of tumor necrosis factor-α and interleukin-1β decreased. Simultaneously, the extent of edema in the hippocampus and frontal lobe decreased, and the morphology of the nerve cells recovered to near normal. In addition, fluoxetine treatment displayed a similar effect on post-stroke depression as electroacupuncture at GB 12 acupoint. The results indicate that electroacupuncture at GB 12 acupoint can reduce the levels of cytokines in the hippocampus and frontal lobe of rats with post-stroke depression, and thus provide a neuroprotective effect on post-stroke depression.
Zhang L.,Liaoning Medical College |
Sui R.-B.,Renmin University of China
Cellular Physiology and Biochemistry | Year: 2017
Objective: To study the metabolic changes of cerebellum by proton magnetic resonance Spectroscopy (1H-MRS) and discuss the relationships between the cerebellar changes and depression severity in patients with post-stroke depression. Methods and Results: Data of demographic characteristics, individual history and life style of all subjects were collected. 40 patients with stroke and 20 controls were enrolled. All groups received T1WI, T2WI, DWI and 1H-MRS examination. The cerebral infarction volume and the distribution and severity of leukoaraiosis were evaluated. The ratios of NAA/Cr, Cho/Cr and Cho/NAA in the cerebellum were calculated. There were no statistical significant difference in the NAA/Cr, Cho/Cr and Cho/NAA ratios in bilateral cerebellum between CONT group and NORM group. The Cho/Cr and Cho/NAA ratios in the cerebellum contralateral to the stroke region were higher in PSD group than those in NORM and CONT groups, and the Cho/Cr and Cho/NAA ratios in the cerebellum ipsilateral to the stroke region were similar with those in NORM and CONT groups. However, there were no statistical significant difference in the NAA/Cr ratios in bilateral cerebellum among three groups. Conclusion: The result shows preliminarily that the cerebellum involves in the development of post-stroke depression. © 2017 The Author(s). Published by S. Karger AG, Basel.
Cheng W.,Liaoning Medical College |
Li Z.,Shenyang University
Chinese Pharmacological Bulletin | Year: 2010
Aim: To investigate the changes of [Ca2+]i in the pulmonary artery smooth muscle cells of PAH rats induced by MCT. Methods: PAH rat model was established by MCT intraperitoneal injection. The PASMCs were primarily cultured and loaded with Fura-2/AM. Effects of Ryanodine receptor agonists on intracellular calcium were measured by Fluorescence microscopy Results: After being given 10 nmol·L-1 Ryanodine, the concentration of [Ca2+]i in control group increased by (93.31 ± 12.41) nmol·L-1; and the concentration in PAH group increased by ( 141.71 ± 13.59) nmol·L-1 (P < 0.01); 10 mmol·L-1 Caffine could increase [Ca 2+]i in control group by (149.02 ± 13.02) nmol·L-1, and increase [Ca2+]i in PAH group by (191.2 ± 21.26) nmol·L-1 (P < 0.01). Conclusion: The results demonstrate that the sensitivity of PASMCs of PAH rats to RyR agonist increase markedly, and suggest that the RyR Ca2+ release probably is abnormal in pulmonary artery smooth muscle cells of PAH rats.
Zhang J.,Liaoning Medical College |
Tao W.,Liaoning Medical College
International Journal of Biological Macromolecules | Year: 2015
This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. © 2013.
Sui R.,The First Affiliated Hospital |
Zhang L.,Liaoning Medical College
Neurological Research | Year: 2011
Objective: To explore the risk factors for stroke-associated pneumonia (SAP). Methods: A retrospective research study was carried out to investigate the clinical data of 1435 patients admitted to the neurological intensive care unit at our university hospital between 1 January 2000 and 31 December 2009. Results: A multi-factorial analysis produced the following results: (1) SAP is 1.113 times more likely to occur for each 1-year increase in age; (2) diabetic patients are 1.612 times more likely to develop SAP than nondiabetic patients; (3) the incidence of SAP decreases by a factor of 0.890 with a one-point increase in the Glasgow coma scale score; (4) nasal feeding patients are 4.981 times more likely to develop SAP than nonnasal feeding patients; (5) patients who use H2-receptor blocking agents are 2.837 times more likely to develop SAP than those who do not; (6) patients who preventively use antibiotics are 2.675 times more likely to develop SAP than those who do not; (7) patients whose hospitalization periods are.> 20 days are 0.500 times more likely to develop SAP than those who do not; (8) patients who suffer from tracheal intubation are 2.980 times more likely to develop SAP than those who do not; and (9) patients who suffer from tracheal incision are 2.190 times more likely to develop SAP than those who do not. Conclusions: SAP was more closely related with diabetes, age, consciousness, days of hospitalization, tracheal intubation, tracheal incision, nasal feeding treatment, and the application of H2-receptor blocking agents and antimicrobials. © W. S. Maney & Son Ltd 2011.
Wang J.,Guangdong Medical College |
Yang J.,Liaoning Medical College |
Zou Y.,Guangdong Medical College |
Huang G.-L.,Guangdong Medical College |
He Z.-W.,Guangdong Medical College
Asian Pacific Journal of Cancer Prevention | Year: 2013
A number of studies have indicated that Nurr1, which belongs to a novel class of orphan nuclear receptors (the NR4A family), is important for carcinogenesis. Here we investigated expression of Nurr1 protein in benign and malignant human prostate tissues and association with clinicopathologic features using immunohistochemical techniques. Moreover, we also investigated the ability of Nurr1 to influence proliferation, migration, invasion and apoptosis of human prostate cancer cells using small interfering RNA silencing. Immunohistochemical analysis revealed that the expression of Nurr1 protein was higher in prostate cancer tissues than in benign prostate tissue (P < 0.001), levels being positively correlated with tumor T classification (P = 0.003), N classification (P = 0.017), M classification (P = 0.011) and the Gleason score (P = 0.020)of prostate cancer patients. In vitro, silencing of endogenous Nurr1 attenuated cell proliferation, migration and invasion, and induced apoptosis of prostate cancer cells. These results suggest that Nurr1 may be used as an indicator for prostate cancer progression and be useful for novel potential therapeutic strategies.
Tian B.,Liaoning Medical College |
Zhang Y.,Jinzhou Central Hospital |
Zhang J.,Liaoning Medical College
Tumor Biology | Year: 2014
The objective of this study is to investigate the expression level of periostin in cancer stem cells as well as in the glioma tissues and the relationship between periostin expression and clinical and pathological characteristics and prognosis of gliomas. ESA+/CD133+/lin- tumor cells were selected by flow cytometry from glioma tissues, and the periostin expression in ESA+/CD133+/lin- tumor cells and non-ESA+/CD133+/lin- tumor cells was detected by quantitative real-time polymerase chain reaction (RT-PCR) and Western blot analysis. The expression status of periostin in glioma tissues was analyzed by immunohistochemistry staining, and the relationship between periostin and clinicopathological parameters of gliomas was determined. It showed that periostin is expressed higher in ESA+/CD133+/lin- tumor cells compared to non-ESA+/CD133+/lin- tumor cells in both mRNA and protein levels. One hundred eighteen (37.82 %) glioma patients were observed with highly expressed periostin protein in immunohistochemistry. Moreover, we observed that the expression of periostin protein was related to Karnofsky performance scale score (KPS), extent of resection, Ki67, and WHO grade of gliomas in universal analysis (P=0.008, 0.045, 0.001, and 0.001, respectively). However, only WHO grade was identified to be related to periostin expression in gliomas after multivariate analysis. After survival analysis, the cases with highly expressed periostin protein attained a significantly poorer postoperative disease-specific survival and distant metastasis than those with none/low expressed periostin protein (P=0.001 and 0.002). In the Cox regression test, KPS, extent of resection, Ki67, WHO grade, and periostin were detected as the independent prognostic factors (P=0.008, 0.007, 0.032, 0.001, and 0.001, respectively). Periostin can be an important prognostic marker for gliomas, which may present a new therapeutic target for glioma patients. © 2014 International Society of Oncology and BioMarkers (ISOBM).
Lei T.,Liaoning Medical University |
Ling X.,Liaoning Medical College
World Journal of Gastroenterology | Year: 2015
AIM: To investigate the molecular mechanisms of the high IGF-1 level linking diabetes and cancers, which is a risk factor. METHODS: We used cell growth, wound healing and transwell assay to evaluate the proliferation and metastasis ability of the hepatocellular carcinoma (HCC) cells. Western blot and reverse transcription polymerase chain reaction were used to assess a previously identified lysosomal protease, cathepsin B (CTSB) expression in the HCC cell lines. C57 BL/6J and KK-Ay diabetic mice are used to detect the growth and metastasis of HCC cells that were depleted with or without CTSB shRNA in vivo . Statistical significance was determined by Student's t-test. RESULTS: IGF-1 promoted the growth and metastasis of the HCC cell lines via its ability to enhance CTSB expression in both a time-dependent and concentration-dependent manner. HCC cells grew much faster in diabetic KK-Ay mice than in C57 BL/6J mice. Additionally, more metastatic nodules were found in the lungs of KK-Ay mice than the lungs of C57 BL/6J mice. CTSB depletion protects against the tumorpromoting actions of IGF-1 in HCC cells, as well tumor growth and metastasis both in vitro and in vivo . IGF-1 did not change the mRNA levels of CTSB but prolonged the half-life of cathepsin B in Hepa 1-6 and H22 cells. Our results showed that IGF-1 promotes the growth and metastasis of the HCC cells most likely by hindering CTSB degradation mediated by the ubiquitinproteasome system (UPS), but not autophagy. Overexpression of proteasome activator 28, a family of activators of the 20S proteasome, could not only restore IGF-1-inhibited UPS activity but also decrease IGF-1-induced CTSB accumulation. CONCLUSION: Our study demonstrates that IGF-1 promotes the growth and metastasis of hepatocellular carcinoma by inhibition of proteasome-mediated CTSB degradation. © The Author(s) 2015.
Xu J.,First Affiliated Hospital of Liaoning Medical College |
Li H.,Liaoning Medical College |
Gu L.,First Affiliated Hospital of Liaoning Medical College |
Zhou X.,First Affiliated Hospital of Liaoning Medical College
Tumor Biology | Year: 2014
Vitamin D receptor (VDR) poly(A) is a common genetic polymorphism in the VDR gene, and it has been implicated to be associated with breast cancer risk. However, previous studies on the association reported inconclusive results. We performed this meta-analysis to comprehensively assess the association. Eligible studies were searched in PubMed and EMBASE databases. Odds ratio (OR) and its 95 % confidence interval (95 % CI) were used for statistical analysis. A total 6,631 cases and 6,718 controls from 11 case-control studies were finally included into the meta-analysis. Meta-analysis of total eligible studies showed that VDR poly(A) polymorphism was not associated with the risk of breast cancer (S versus L: OR=0.99, 95 % CI of 0.90-1.09, P=0.84; SS versus LL: OR=0.96, 95%CI of 0.79-1.18, P=0.70; SS/LS versus LL: OR=0.96, 95%CI of 0.83-1.12, P=0.63; SS versus LL/LS: OR=1.00, 95 % CI of 0.91-1.10, P=0.98). Metaanalysis of studies with high quality also showed that there was no association between VDR poly(A) polymorphism and breast cancer risk. In addition, in the subgroup analysis by ethnicity, no significant association was found among Caucasians. Therefore, the meta-analysis suggests that VDR poly(A) polymorphism is not associated with the risk of breast cancer. Large well-designed studies are necessary to clarify the possible association in Asians.
Yuan C.L.,Liaoning Medical College
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2013
To study the preparation of total flavones in Glechoma longituba sustained-release tablets and evaluate its releasing features in vitro. Orthogonal experiment (L(9)3(4)) was used to optimize the process of preparation of total flavones in Glechoma longituba sustained-release tablets. Investigated the release effect in vitro of optimization test result. The optimized prescription was HPMC-K4M and carbopol 934p account for 30% of the total tablet weight, their dosage ratio was 2: 1; Lactose as additives, 20% dosage; 5% PVP ethanol as adhesives, and compressed the wet granule of the materials into the total flavones in Glechoma longituba sustained-release tablets. The released profiles of the sustained-release tablets followed zero-order equation. The sustained-release effect of total flavones in Glechoma longituba sustained-release tablets is good, the preparation craft is easy and feasible and worth widely promoting.