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Francis W.R.,University of Swansea | Owens S.E.,University of Swansea | Wilde C.,University of Swansea | Pallister I.,University of Swansea | And 4 more authors.
Biochemical and Biophysical Research Communications | Year: 2014

During bone maintenance in vivo, estrogen signals through estrogen receptor (ER)-α. The objectives of this study were to investigate the temporal expression of ERα36 and ascertain its functional relevance during osteogenesis in human bone marrow derived stromal cells (BMSC). This was assessed in relation to runt-related transcription factor-2 (runx2), a main modulatory protein involved in bone formation. ERα36 and runx2 subcellular localisation was assessed using immunocytochemistry, and their mRNA expression levels by real time PCR throughout the process of osteogenesis. The osteogenically induced BMSCs demonstrated a rise in ERα36 mRNA during proliferation followed by a decline in expression at day 10, which represents a change in dynamics within the culture between the proliferative stage and the differentiative stage. The mRNA expression profile of runx2 mirrored that of ERα36 and showed a degree subcellular co-localisation with ERα36. This study suggests that ERα36 is involved in the process of osteogenesis in BMSCs, which has implications in estrogen deficient environments. © 2014 Elsevier Inc. All rights reserved. Source


Shi D.,Liaoning Normal University | Liu Y.,Liaoning Normal University | Lian X.,Liaoning Normal University | Zou W.,Liaoning Normal University | Zou W.,Liaoning Key Laboratories of Biotechnology and Molecular Drug Research and Development
Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology | Year: 2013

Caveolae are specialized lipid rafts that form flask-shaped invaginations of the plasma membrane. Many researches show that caveolae are involved in cell signaling and transport. Caveolin-1 is the major coat protein essential for the formation of caveolae. Recently, several reports indicated that the other caveolae-associated proteins, Cavins, are required for caveola formation and organization. It's worth noting that Cavin-1 could cooperate with Caveolin-1 to accommodate the structural integrity and function of caveolae. Here, we reviewed that the relationship between Cavins and Caveolins and explore the role of them in regulating caveolae. © 2013 by the Institute of Microbiology, the Chinese Academy of Sciences and the Chinese Society for Microbiology. Source


Liu Y.,Liaoning Normal University | Wang Y.,Liaoning Normal University | Shi D.,Liaoning Normal University | Zou W.,Liaoning Normal University | Zou W.,Liaoning Key Laboratories of Biotechnology and Molecular Drug Research and Development
Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology | Year: 2012

Autophagy, is an essential cellular process involving self-degradation of intracellular components via the lysosome, which plays the Janus role in cancer initiation and progression. Caveolin-1, a marker protein of caveolae, functions as scaffolding protein mediating many physiological and pathological processes including caveolae biogenensis, vesicular transport, cholesterol homeostasis, signal transduction and tumorigenesis. Recently, many reports showed that autophagy of tumor cells associated with stromal Cav-1. We reviewed that the relationship between autophagy and Cav-1 involved in tumorigenesis and development. © 2012 Chin J Biotech. All Reserved. Source


Wang X.,Liaoning Normal University | Feng S.,Liaoning Normal University | Zhang H.,Liaoning Normal University | Wang Y.,Liaoning Normal University | And 5 more authors.
Molecular Biology Reports | Year: 2011

Several evidences support that caveolin-1 is associated with mammary cell transformation and oncogenesis. We have previously reported that a cell line, named MCF10A-ST1 (ST1), which only expressed 30% Cav-1 compared with parental cells MCF10A (human mammary epithelial cell line) was isolated by gene trapping. The decreased expression of Cav-1 is sufficient for phenotypic transformation of MCF10A cells, which involved in the loss of anchorage-dependent growth and migration in nude mice with the existence of E2. The previous study in our lab on microarray assay showed that the expression of cyclin D1 (cell cycle protein) was up-regulated in ST1 cell line. Here, we not only confirmed the results by Western Blot but also demonstrated that Cav-1 down-regulation accelerate the progression of mammary cells from G1 phase into S phase by Flow Cytometry (FCM). This proposed that the Cav-1 down-regulation could change the progress of cell cycle in the mammary cells. Otherwise, microarray assay also showed that the transcription factor (c-Jun) was up-regulated. But, the original carcinoma gene (c-Fos) and transcription factor (AP-1) have not obviously changed compared with MCF10A. ST1 cells obtained the morigenicity in nude mice with the existence of E2, and the immunoprecipitation showed the interactions of Cav-1 with ERα in both MCF10A and ST1. ERα expression was increased as further down-regulation of Cav-1. So, we hypothesize that Cav-1 down-regulation could induce the activation of ERα-associated signaling pathway, in order to adjust the development and proliferation. By siRNA technology, the down regulation of Cav-1 could activate MAP kinase and Akt signaling pathway, including the phosphorylation of ERK1/2 and Akt. However, the mechanism of Cav-1 down-regulation in the early transformation and signaling transduction of mammary epithelial cells is unclear. Here, we report that down-regulation of caveolin-1 protein expression leads to deregulate estrogen receptor alpha (ERα) signaling and consequently early transformation in mammary epithelia. © 2010 Springer Science+Business Media B.V. Source


Liu J.,Dalian University of Technology | Liu J.,Liaoning Normal University | Qu C.,Liaoning Normal University | Li H.,Liaoning Normal University | And 9 more authors.
Journal of Membrane Biology | Year: 2016

Voltage-gated potassium (Kv) channels are involved in the proliferation and transformation of mammary epithelial cells. They are thought to be related to the development of breast carcinoma, although the exact role they play in this event remains unclear. In this study, we investigated whether the expression and function of Kv channels is associated with Caveolin-1 (Cav-1, a principal component of caveolae) in different cell lines. We found that expression of Cav-1 correlated with the expression of Kv channels in mammary epithelial cells (MCF10A, MCF10A-ST1, and MCF7), and silencing of Cav-1 inhibited the expression of KCNA5 (voltage-gated shaker-related subfamily A, member 5). Immunofluorescence analysis indicated the colocalization of KCNA5 with Cav-1, whereas immunoprecipitation suggested a possible interaction between the two proteins. Overall, our finding indicated that KCNA5 protein may interact with Cav-1, thereby contributing to the proliferation and early transformation of mammary cells. © 2016 Springer Science+Business Media New York Source

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