Hou C.-J.,General Hospital of Shenyang Military Region of PLA |
Qi Y.-M.,Liaoning Jinqiu Hospital |
Zhang D.-Z.,General Hospital of Shenyang Military Region of PLA |
Wang Q.-G.,General Hospital of Shenyang Military Region of PLA |
And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013
Background: New wound in the border of defected hearts can promote self-healing, and stromal cell-derived factor-1 alpha can promote angiogenesis and cardiac function. Objective: To investigate the effects of mechanical injury and stromal cell-derived factor-1 alpha on the proliferation of cardiomyocytes and the effects of cardiomyocytes on chemotactic migration of cardiac fibroblasts. Methods: Rat cardiomyocytes and cardiac fibroblasts were cultured primarily. Rat cardiomyocytes were injured mechanically by scratching, and treated with 10-160 μg/L stromal cell-derived factor-1 alpha. The Cell Counting Kit-8 assay was employed to evaluate the proliferation of cardiomyocytes and the Transwell migration assay was used to detect the chemotaxis and migration capability of cardiac fibroblasts. Results and Conclusion: Under mechanical injury condition, stromal cell-derived factor-1 alpha significantly promoted the growth of cardiomyocytes with the maximum effect occurring at 80 μg/L. In addition, mechanically injured and stromal cell-derived factor-1 alpha cultured cardiomyocytes remarkably promoted the migration of cardiac fibroblasts in a time-dependent fashion. These findings indicate that stromal cell-derived factor-1 alpha could promote the growth of cardiomyocytes under mechanical injury condition; otherwise, mechanically injured and stromal cell-derived factor-1 alpha cultured cardiomyocytes could promote the migration of cardiac fibroblasts.
Chen H.,Liaoning Jinqiu Hospital |
Ma F.,Zhejiang University |
Hu X.,Liaoning Medical University |
Jin T.,Liaoning Medical University |
And 2 more authors.
Biochemical and Biophysical Research Communications | Year: 2013
Increased systemic level of inflammatory cytokines leads to numerous age-related diseases. In senescent macrophages, elevated prostaglandin E2 (PGE2) production contributes to the suppression of T cell function with aging, which increases the susceptibility to infections. However, the regulation of these inflammatory cytokines and PGE2 with aging still remains unclear. We have verified that cyclooxygenase (COX)-2 expression and PGE2 production are higher in LPS-stimulated macrophages from old mice than that from young mice. Downregulation of RXRα, a nuclear receptor that can suppress NF-κB activity, mediates the elevation of COX2 expression and PGE2 production in senescent macrophages. We also have found less induction of ABCA1 and ABCG1 by RXRα agonist in senescent macrophages, which partially accounts for high risk of atherosclerosis in aged population. Systemic treatment with RXRα antagonist HX531 in young mice increases COX2, TNF-α, and IL-6 expression in splenocytes. Our study not only has outlined a mechanism of elevated NF-κB activity and PGE2 production in senescent macrophages, but also provides RXRα as a potential therapeutic target for treating the age-related diseases. © 2013.
Yu L.,Liaoning University of Traditional Chinese Medicine |
Zhang M.,Liaoning Jinqiu Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: Increasing basic researches have confirmed that human umbilical cord blood stem cells is safe and effective to treat acute myocardial infarction complicated by heart failure, but this method is not yet available for clinical use. OBJECTIVE: To observe the long-term effect of human umbilical cord blood mononuclear cell transplantation in the treatment of myocardial infarction combined with heart failure. METHODS: Twenty-five patients with myocardial infarction and heart failure admitted in the Department of Cardiology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, from January 2009 to June 2011 were randomly assigned to receive intracoronary transplantation of human umbilical cord blood mononuclear cells in addition to conventional therapy (transplantation group, n=12) or standard therapy (control group, n=13). All patients underwent standardized drug therapy, and coronary angiography and percutaneous coronary intervention were performed at acute phase. Improvement in heart function, left ventricular ejection fraction, left ventricular end-systolic volume and left ventricular end-diastolic volume at baseline and 12, 24 months after treatment were monitored. RESULTS AND CONCLUSION: The left ventricular ejection fraction, left ventricular end-systolic volume and left ventricular end-diastolic volume were significantly improved 12, 24 months after human umbilical cord blood stem cell transplantation compared to baseline (P < 0.05), while these parameters remained unchanged in the control group (P > 0.05). Compared with the control group, the left ventricular ejection fraction was increased significantly, and the left ventricular end-systolic volume and left ventricular end-diastolic volume were reduced significantly in the transplantation group. During the follow-up, no side effects were observed. These findings indicate that human umbilical cord blood mononuclear cell transplantation leads to significant and longstanding improvements in left ventricular performance of patients with myocardial infarction and heart failure. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
Weng L.,Peking Union Medical College |
Joynt G.M.,Chinese University of Hong Kong |
Lee A.,Chinese University of Hong Kong |
Du B.,Peking Union Medical College |
And 19 more authors.
Intensive Care Medicine | Year: 2011
Introduction: Critical care doctors are frequently faced with clinical problems that have important ethical and moral dimensions. While Western attitudes and practice are well documented, little is known of the attitudes or practice of Chinese critical care doctors. Methods: An anonymous, written, structured questionnaire survey was translated from previously reported ethical surveys used in Europe and Hong Kong. A snowball method was used to identify 534 potential participants from 21 regions in China. Results: A total of 315 (59%) valid responses were analysed. Most respondents (66%) reported that admission to an intensive care unit (ICU) was commonly limited by bed availability, but most (63%) would admit patients with a poor prognosis to ICU. Only 19% of respondents gave complete information to patients and family, with most providing individually adjusted information, based on prognosis and the recipient's educational level. Only 28% disclosed all details of an iatrogenic incident, despite 62% stating that they should. The use of do not resuscitate orders or limitation of life-sustaining therapy in terminally ill patients reported as uncommon and according to comparable reports, both are more common practice in Hong Kong or Europe. In contrast to European practices, doctors were more acquiescent to families in decision-making at the end of life. Conclusions: A number of differences in ethical attitudes and related behaviour between Chinese, Hong Kong and European ICU doctors were documented. A likely explanation is differing cultural background, and doctors should be aware of likely expectations when treating patients from a different culture. © 2011 Copyright jointly held by Springer and ESICM.
Song M.,General Hospital of Shenyang Military Command |
Bao J.,Liaoning Jinqiu Hospital |
Wang X.,General Hospital of Shenyang Military Command |
Li S.,General Hospital of Shenyang Military Command
European Archives of Oto-Rhino-Laryngology | Year: 2015
The polysomnography after nasopharyngeal tube insertion (NPT-PSG) was used to assess glossopharyngeal airway obstruction in patients with moderate-to-severe obstructive sleep apnea-hypopnea syndrome (OSAHS), and was compared with that obtained using spiral computed tomography (CT). A total of 125 patients diagnosed with moderate-to-severe OSAHS using PSG were subjected to PSG after NPT insertion, and spiral CT was used for continuous scan of the upper respiratory tract. The NPT-PSG and CT scan results were subjected to correlation analysis. In addition, the two sets of data were used separately to determine whether there was glossopharyngeal airway stenosis or obstruction, and the results were compared. Neither apnea hypopnea index (AHI) nor lowest oxygen saturation (LSaO2) obtained from the first PSG was significantly correlated with the cross-sectional area or the inner diameter of the glossopharyngeal airway. NPT-PSG AHI was significantly correlated with the cross-sectional area and anteroposterior diameter of the glossopharyngeal airway, with correlation coefficients of 0.264 and 0.185, and P values of 0.003 and 0.039, respectively. NPT-PSG AHI was not significantly correlated with the left–right diameter of the airway, and NPT-PSG LSaO2 was not significantly correlated with the cross-sectional area or the inner diameter of the glossopharyngeal airway. With NPT-PSG, 52 patients showed obvious glossopharyngeal airway stenosis while 73 patients did not, and with CT scan 41 patients showed obvious glossopharyngeal airway stenosis while 84 patients did not. The two methods reached the same diagnosis in 86 cases, with a consistency rate of 68.8 %. Spiral CT and NPT-PSG show certain degrees of consistency by assessing the presence of glossopharyngeal airway stenosis or obstruction, and there are also notable differences. Clinical assessment on glossopharyngeal airway obstruction should be based on a combination of multiple methods. © 2015, Springer-Verlag Berlin Heidelberg.