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Li Y.,ShenYang Agricultural University | Li L.,Shijiazhuang Hongyi Energy Saving and Environmental Protection Technology Co. | Zhang D.,Liaoning Institute of Energy Resource | Gu S.,ShenYang Agricultural University | And 2 more authors.
Nongye Jixie Xuebao/Transactions of the Chinese Society of Agricultural Machinery | Year: 2012

Based on the early single factor test, quadratic regression orthogonal rotating combination design was applied. The whole gas production was taken as response value. Effects of four factors on anaerobic fermentation were studied. These four factors are the ratio of food garbage and cow dung, temperature, pH value and the ratio of inoculum and quality of fermentation. The mathematical mode about mixed fermentation of food garbage and cow dung was established, regression equation was optimized and analyzed, in addition, the optimal condition and interactive effects were exposed. The optimum technological conditions for the mixed-fermentation was as the followings: the ratio of food garbage and cow dung was 2.5, temperature was 37.5°C, the pH value was 7.0 and the ratio of inoculum volume and quality of fermentation was 4.The order of the influence of four factors on mixed-fermentation of food garbage and cow dung was as follows: the ratio of inoculum and quality of fermentation, temperature, the ratio of food garbage and cow dung, and pH value. The gas production of the optimum technological condition and the random experiment also showed no significant difference with the predictive value. The model fitted well. Source


Kou W.,ShenYang Agricultural University | Kou W.,Liaoning Institute of Energy Resource | Cao Y.,Liaoning Institute of Energy Resource | Zhang D.,Liaoning Institute of Energy Resource | And 3 more authors.
Taiyangneng Xuebao/Acta Energiae Solaris Sinica | Year: 2014

Analysis the reducing sugar conversion characteristic of corn straw which has been treated once and twice machine expansion process. Scanning electron microscopy (SEM) microscopic surface structure showed that the structure of the straw significantly has been loosed after expansion treatment; X Ray Diffraction analysis showed that the crystallization degree of once and twice machine expansion material decreases 14.14% and 21.61%, respectively. Infrared spectral analysis cellulose, hemicelluloses and lignin chemical groups, the characteristics of their structure have all changed certainly. Further enzymolysis sugar conversion testing showed that the once and twice machine expansion material's reducing sugar productivity are 0.24 g/g and 0.29 g/g, respectively. Analyzed enzymolysis sugar liquid of twice expansion material by Ion Chromatography concluded that glucose is 72.12% in total sugar, and xylose is 22.63%. Calculation showed the cellulose sugar conversion is about 70%, hemicelluloses sugar conversion is about 30%. Source


Liu Q.,Liaoning Institute of Energy Resource | Sun Y.,Liaoning Institute of Energy Resource | Kou W.,Liaoning Institute of Energy Resource | Cao Y.,Liaoning Institute of Energy Resource | And 3 more authors.
Chinese Journal of Environmental Engineering | Year: 2013

The compositions and properties of municipal solid waste(MSW) vary with the seasons and human hobbies. The composition characteristics of municipal solid waste in different seasons of northern cities were studied, the corresponding components of TOC, TN, protein, fat, reducing sugar, etc. were analyzed. Through the anaerobic digestion experiment, the varying pattern of pH, daily gas production, methane content, methane cumulant, VFAs, ORP of MSW, were tested, in which the influencing factors of the corresponding change were analyzed. The results show that the water contents of the second and third quarter are 64.81% and 67.50%, respectively, higher than that of the first and fourth quarter. The protein and fat contents in fermenting materials of first quarter are 12.56% and 8.86%, respectively, obviously higher than that of the other quarters. The methane cumulant and gas production by unit fermentation raw materials of first quarter are 17 616 mL and 204.8 mL/g, respectively, which is also the highest in four quarters. It indicated that the protein and fat have an important impact on process and results of anaerobic fermentation. The research could provide some reference and demonstration for anaerobic digestion projects of MSW. Source


Lun X.,Shenyang Aerospace University | Kou W.,Liaoning Institute of Energy Resource | Zhao Y.,Liaoning Institute of Energy Resource | Gu S.,ShenYang Agricultural University | And 2 more authors.
Chinese Journal of Environmental Engineering | Year: 2013

The corn straw was pretreated by expansion technology. Orthogonal experiment was designed by choosing conversion rate of reducing sugar as influence value basing on the single factor experiment. The influences of temperature, pH, liquid-solid ratio, enzyme concentration and time on the cellulose enzyme solution process were studied. Results showed that the optimum conditions were as follows: enzymolysis temperature of 48°C, pH of 4.5, liquid-solid ratio of 8:1, enzyme concentration of 36.0 U/g, enzymolysis time of 25 h. The conversion rate of reducing sugar is 28.98% under the conditions. The enzymolysis of cellulose of corn straw is complete after expansion pretreatment by scanning electron microscopy (SEM). The characteristic peak of cellulose in enzymolysis process changes obviously after expansion pretreatment by infrared spectrum. Source


Shao L.,Liaoning Institute of Energy Resource | Kou W.,Liaoning Institute of Energy Resource | Cao Y.,Liaoning Institute of Energy Resource | Wu C.,Shenyang Aerospace University | And 2 more authors.
Chinese Journal of Environmental Engineering | Year: 2014

Cellulose and hemicellulase were used to degrade the corn straw pretreated by expanded in order to improve the sugar yield which compared with degraded only by cellulose. On basis of single factor experiment, and use reducing sugar yield as response value, optimization experiment was designed by response surface. By analysis of testing data, results showed that the best process for the expanded corn straw enzymatic hydrolysis was pH of 4.8, liquid to solid ratio of 13:1, hydrolysis time of 60 h, enzyme concentration of 6 g/L, temperature of 51℃. Compared with the results which degraded only by cellulase, the reducing sugar yield increased 14.3% and reached to 24%.The study of synergetic enzymatic hydrolysis provides a new way to degrade the lignocellulose material. Source

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