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Xu S.,ShenYang Agricultural University | Li K.,ShenYang Agricultural University | Xie H.,Liaoning Economic Management Cadre College | Liu Z.,ShenYang Agricultural University | And 2 more authors.
Pakistan Journal of Botany

The aim of the present work was to screen out grape germplasms resisting to replant obstacle, and to analyze their resistant mechanism. Here we used 94 grape resources as the testing materials. The cuttings of each resource were planted in pot filled with control (normal) soil as well as replanting soil. After 2 years investigation, ‘101-14’, ‘8612’were screened for replant-susceptible resources, and ‘Mcadams’, ‘Dawuhezi’ were screened for replant-resisting resource. Under replanting stress, resources with resistance exhibited an increase in maximum photochemical efficiency of PS�, and net photosynthetic rate improved. The MDA content of ‘Mcadams’ planted in normal soil was 25.62% lower than that planted in replant soil, and showed a strong resistance. For ‘Dawuhezi’, the protected enzyme SOD and PPO could be activated under replanting stress, which effectively avoided the harm of active oxygen to the seedling, presenting a more vigorous plant growth. © 2015, Pakistan Botanical Society. All rights reserved. Source

Li K.,ShenYang Agricultural University | Guo X.W.,ShenYang Agricultural University | Xie H.G.,Liaoning Economic Management Cadre College | Guo Y.,ShenYang Agricultural University | Li C.,ShenYang Agricultural University
Pakistan Journal of Botany

The effects of grape root exudates and residues on rhizosphere available nutrient, enzyme activity, microorganism quantity and population structure were determined with potted seedlings of Beta (V. vulpine x V. labrusca) as materials. Plant growth was suppressed and available P and K contents in rhizosphere soil decreased, while alkaline hydrolysis N content increased under high concentration of root exudates. As the addition ratio of root residues increased in soil, the contents of available N, P and K in rhizosphere soil increased. The correlation was found between alkaline hydrolysis N content and urease activity under the treatment of root exudates and residues, but the activity of invertase was weakened and had no obvious correlation with soil nutrient or plant growth. Polyphenol oxidase activity was also weakened when the concentration of root exudates and residues increased. The rhizosphere soil was converted from 'bacteria type' to 'fungi type' under root exudates of high concentration. Under the treatment of root residues, both bacteria/fungi and actinomycetes/fungi ratio increased in rhizosphere soil, however, fungi population diversity reduced. The beneficial trichoderma disappeared, while it appeared with pythium, thielaviopsis and Stilbellales. The results showed that the change of available N, P and K content was not the major reason that led to the depression of plant growth, while the alternation of polyphenol oxidase activity and the change of microorganism quantity and population structure might be the important reason of grape replant disease. Source

Guo X.-W.,ShenYang Agricultural University | Wang B.,ShenYang Agricultural University | Li K.,ShenYang Agricultural University | Liu Z.-D.,ShenYang Agricultural University | And 4 more authors.
Biotechnology and Biotechnological Equipment

Phenolic compounds are considered to be an important autotoxic substances. The interactions between them and soil microbes are increasingly becoming a focus of the allelopathic research studies. In the present study, we have evaluated the effect of 4-hydroxybenzoic acid on soil microbial community structure and functional diversity. Bulk soil without grapevine cuttings (Group 1) and rhizosphere soil with Beta (Vitis riparia £ Vitis labrusca) grapevine cuttings (Group 2) were used as research subjects. The bacterial (16s rRNA) and fungal internal transcribed spacer regions community structures were obtained using a denaturing gradient gel electrophoresis and substrate utilization patterns were determined using Biolog EcoPlates. In this research, the results showed that the 4-hydroxybenzoic acid caused a shift in the soil bacterial and fungal community structures and changed the soil microbial functional diversity. The results also showed that the diversity of the microbial community structure and function differed between the two designated groups. The application of 4-hydroxybenzoic acid (1 and 2 mg/g) to the soil resulted in a greater decrease in the average diversity of the bacterial community structure in Group 2, compared to Group 1. Moreover, the average diversity of the fungal community structure in Group 2 became higher than that in Group 1. However, the bacterial and fungal community structural diversities increased with the application of 0.5 mg/g of 4-hydroxybenzoic acid. Thus, we concluded that both soil microbes and root exudates might be the recipients of 4-hydroxybenzoic acid. ©2015 The Author(s). Published by Taylor & Francis. Source

Wang B.,ShenYang Agricultural University | Guo X.W.,ShenYang Agricultural University | Li K.,ShenYang Agricultural University | Han X.,ShenYang Agricultural University | And 4 more authors.
Allelopathy Journal

We studied the allelopathic effects of salicylic acid (SA) on grape vine (Vitis vinifera L.) plants, its content in grape root exudates and effects on soil microbial community. Grape vine roots secrete SA but its concentration in the root exudates decreases, when the plants were exposed to exogenously added SA. Lower concentrations of exogenously added SA, stimulated the vine growth but was inhibitory at higher concentrations. There was no direct correlation between the inhibitory effects of SA on plant growth and the diversity of soil bacterial or fungal community structure. Exogenously added SA changed the root exudate composition, which further influenced the soil bacterial metabolic activity, and changed the functions of bacterial communities, which might be related to plant growth. © 2015, International Allelopathy Foundation. All rights Reserved. Source

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