Shenyang, China
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Li L.-C.,Liaoning Blood Center | Li J.-P.,Liaoning Blood Center
Chinese Journal of Tissue Engineering Research | Year: 2014

Background: Bone marrow mesenchymal stem cells have become a promising kind of target cells for gene therapy because of easy preparation, strong self-proliferative capacity, stable biological characteristics, low immunogenicity, long-term survival in the host brain, easy transfection by exogenous gene, high transfection efficiency, and tropism for tumor cells. Objective: To analyze the advantages and disadvantages of mesenchymal stem cells used in gene therapy for refractory diseases based on different gene transfer methods. Methods: A computer-based online retrieval was performed in PubMed database, Chinese Journal Full Text Database, Wanfang database to search papers regarding mesenchymal stem cells published from January 2000 to December 2013. The key words were "mesenchymal stem cell, gene therapy" in English and Chinese. Totally 2114 articles were retrieved, and finally, 42 articles were included in result analysis. Results and conclusion: There are a variety of gene therapies that can be divided according to gene manipulation: (1) gene correction and gene replacement; (2) gene augmentation and gene inactivation. There are two routes of administration, in vitro and in vivo. As opposed to other cells, bone marrow mesenchymal stem cells can survive in the host brain for a longer time, are easier to be transfected by exogenous genes, and has a tropism for tumor cells. Thus, bone marrow mesenchymal stem cells have become a promising kind of target cells for gene therapy. Mesenchymal stem cell as carriers of the gene therapy have been used in phase III clinical trials for the treatment of graft-versus-host disease and Crohn's disease treatment. In China, some achievements have been harvested in mesenchymal stem cell therapy for refractory diseases. To exploring the optimal therapeutic window for stem cell transplantation, and to find the appropriate gene dosage, the number of transplanted cells and the appropriate timing of treatment are urgent to be solved.


Li G.-J.,Liaoning Medical University | Zhang X.,Liaoning Blood Center
Chinese Journal of Medical Genetics | Year: 2013

Objective: To investigate the molecular characteristics of Bw subgroup of ABO variant. Methods: Two individuals with Bw phenotypes were confirmed by standard serological assays. ABO genotype was analyzed with polymerase chain reaction-sequence specific primer (PCR-SSP) method. Exons 6-7 of B genes were amplified by specific PCR, and the PCR product was subjected to direct sequencing. Results: The probands were identified as Bw phenotype by serological assays. As detected by PCR-SSP, the genotypes of the two probands were both B/O2. Compared with the wild-type B101, the B allele in one individual has harbored a missense mutation (C721T) in exon 7, which resulted in an amino acid substitutions (R241S). In the second individual, the B allele has harbored a missense mutation (C278T) in exon 6, which also resulted in an amino acid substitution (P93L). Conclusion: A Bw03 allele and a Bw12 allele were identified, which resulted in varied intensity of B antigen expression.


Li L.-C.,Liaoning Blood Center | He Z.,Fourth Peoples Hospital of Shenyang | Li J.-P.,Liaoning Blood Center
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Compared to bone marrow-, peripheral blood-, fat- and embryo-derived mesenchymal stem cells, umbilical cord- and placenta-derived mesenchymal stem cells possess advantages including wide source, ease of harvesting, no immunological rejection or ethical compromise.OBJECTIVE: To investigate the methods to in vitro isolate, culture and sub-culture of placenta-derived mesenchymal stem cells, providing experimental basis for clinical application of placenta-derived mesenchymal stem cells.PO. Box 1200, Shenyang 110004 www.CRTER.org. METHODS: A computer-based online retrieval was performed to search papers regarding placenta-derived mesenchymal stem cells published in PubMed database, Chinese Journal Full Text Database, Wanfang database using key words "placenta-derived mesenchymal stem cells, induction and differentiation in vitro". A total of 98 papers were retrieved, and 33 were suitable for final analysis.RESULTS AND CONCLUSION: Placenta-derived mesenchymal stem cells are in the stage of basic research in China, but some progress has been made in basic research and clinical application of placenta-derived mesenchymal stem cells in countries outside China. Placenta-derived mesenchymal stem cells possess advantages including wide source, ease in sample harvesting and no immunological rejection or ethical compromise as well as less pain, fewer infection opportunities and stronger proliferative capacity compared to bone marrow donation or peripheral blood collection. For these reasons, placenta-derived mesenchymal stem cells should be further studied.


Liu X.-Z.,Liaoning Blood Center | Li X.-F.,Liaoning Blood Center | Zhang X.,Liaoning Blood Center | Zhang K.-L.,Liaoning Blood Center | Li J.-P.,Liaoning Blood Center
Tissue Antigens | Year: 2011

A new HLA-B*52 allele differs from B*52:01:01 by a single nucleotide exchange at position 583 where a 'C' to 'T'. © 2011 John Wiley & Sons A/S.


Zhang X.,Liaoning Blood Center | Li J.,Liaoning Blood Center
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics | Year: 2016

OBJECTIVE: To explore the effect of variants of alpha-1,3-N-acetylgalactosaminyltransferase of the ABO gene on A antigen expression.METHODS: Three samples were assayed by serological method. The extracted DNA was genotyped by sequence-specific primer polymerase chain reaction (SSP-PCR) and direct sequencing. To identify haploid of the ABO gene, clones for exons 6 and 7 of the ABO locus were analyzed.RESULTS: All of the three samples were identified as the Ael subgroup, among which were found to carry the Ael08 and O01 alleles, while another was confirmed to harbor the Ael08 and O04 alleles. Compared with the A101 allele, two nucleotide alterations (467C>T and 804insG) at exon 7 have resulted in amino acid substitution P156L and frameshift from position 269.CONCLUSION: 467C>T and 804insG variants of the alpha-1,3-N-acetylgalactosaminyltransferase gene probably underlie the Ael phenotype.


Li J.-P.,Liaoning Blood Center | Li X.-F.,Liaoning Blood Center | Zhang K.-L.,Liaoning Blood Center | Zhang X.,Liaoning Blood Center | Liu X.-Z.,Liaoning Blood Center
Tissue Antigens | Year: 2012

The HLA-B*40:74 allele has two nucleotide changes at positions 103 and 106 of exon 2 from the closest matching allele HLA-B*40:01:01. © 2011 John Wiley & Sons A/S.


PubMed | 196K Seven year Program and Liaoning Blood Center
Type: Journal Article | Journal: Zhongguo shi yan xue ye xue za zhi | Year: 2016

To investigate and identify the molecular and biological characteristics of B305 blood group gene subtypes of a ABO variant.The individual was confirmed by standard serological techniques. The genotyping and sequencing were performed by using polymerase chain reaction-sequence specific primer (PCR-SSP), direct sequencing and gene cloning for Exon 6 and exon 7 of ABO locus respectively.B antigen was detected on red blood cells of the proband and there was anti-A antibody in the serum. The PCR-SSP result showed BO1 phenotype. DNA sequencing showed 261delG, 297A/G, 425T/C, 526C/G, 657C/T, 703A/G, 796C/A, 803G/C and 930G/A heterozygote in exon 6 to exon 7. After cloning and sequencing, 2 alleles B305 and O01 were obtained. The sequence of B305 allele had one nucleotide mutation (T to C) at position 425 compared with that of B101 allele, resulting in an amino acid substitution (M142T).T>C at nt425 of -1,3 galactosyltransferase gene can result in B305 blood group gene subtypes, which reduce the expression of B antigen.


PubMed | Liaoning Blood Center
Type: Journal Article | Journal: HLA | Year: 2016

HLA-B*15:226N has one nucleotide change from HLA-B*15:08:01 where 87Q is changed to a stop codon.


PubMed | Liaoning Blood Center
Type: Journal Article | Journal: HLA | Year: 2016

HLA-B*46:28 has one nucleotide change from HLA-B*46:01:01 where 48R is changed to W.


PubMed | Liaoning University of Traditional Chinese Medicine and Liaoning Blood Center
Type: Journal Article | Journal: Zhongguo shi yan xue ye xue za zhi | Year: 2016

To investigate and indentify the molecular characteristics of a sample serologically identified as ABw subgroup.The individual was confirmed by standard serological techniques. The genotyping and sequencing were performed using polymerase chain reaction-sequence specific primer (PCR-SSP), direct sequencing and gene cloning for exon 6 and exon 7 of ABO locus respectively.Both A and B antigen were detected on red blood cells of the proband and anti-B antibody was detected in his serum. PCR-SSP showed that the sample gene was A1B phemotype. DNA sequencing showed 297A/G, 467C/T, 526C/G, 657C/T, 703A/G, 803G/C and 930G/A heterozygote in exon 6 to exon 7. After cloning and sequencing, 2 alleles A102 and Bw33 were obtained. The sequence of Bw33 allele had one nucleotide change (A to C) at position 796 compared with that of B101 allele. The nucleotide in this B allele at site 796 is a C characteristic of the A form of the allele.A>C at nt796 of -1, 3 galactosyltransferase gene can result in Bw33 phenotype with the anti-B antibody in serum.

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