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Li K.,ShenYang Agricultural University | Li K.,Liaoning Agricultural Vocational and Technical College | Guo X.-W.,ShenYang Agricultural University | Guo Y.-S.,ShenYang Agricultural University | And 5 more authors.
Chinese Journal of Applied Ecology | Year: 2010

Taking the tissue-cultured seedlings of grape cultivar Red Globe as test objects, this paper examined the effects of their root aqueous extracts on seedling' s growth, with the allelochemicals identified by LC-MS. The results showed that 0. 02 g • ml-1 (air-dried root mass in aqueous extracts volume; the same below) , 0. 1 g • ml-1, and 0. 2 g • ml-1 of the aqueous extracts inhibited the growth of the seedlings significantly, and the inhibition effect increased with increasing concentration of the extracts. The identified allelochemicals of the extracts included p-hydroxybenzoic acid, salicylic acid, phenylpropionic acid, and coumaric acid. Pot experiment showed that different concentration (0. 1, 1, and 10 mmol • L-1) salicylic acid and phenylpropionic acid inhibited the seedling' s growth remarkably. With the increasing concentration of the two acids, the plant height, stem diameter, shoot- and root fresh mass, leaf net photosynthetic rate and starch content, and root activity of the seedlings decreased, while the leaf soluble sugar and MDA contents increased. No obvious change pattern was observed in leaf protein content.

Yue X.P.,Northwest University, China | Fang Q.,Northwest University, China | Zhang X.,Liaoning Agricultural Vocational and Technical College | Mao C.C.,Northwest University, China | And 3 more authors.
Asian-Australasian Journal of Animal Sciences | Year: 2013

The aim of this study was to investigate allele frequencies at the CSN1S2 locus in two Chinese dairy goat breeds and the effects of its variation on dairy goat economic traits. Seven hundred and eight goats from Xinong Saanen (XS, n = 268) and Guanzhong (GZ, N = 440) breeds were selected. The milk samples of 268 XS goats were collected during the middle of lactation, body size parameters (708 goats) and daily milk yield (202 goats) were registered. The RFLP (restriction fragment length polymorphism) and SSCP (single strand conformation polymorphism) were used to detect the polymorphisms in CSN1S2. The Hardy-Weinberg (HW) equilibrium and the associations between body size, milk yield and composition and the genotypes were calculated. The results revealed that only A and F CSN1S2 alleles were found in the two Chinese dairy goat breeds. Allelic frequencies of A and F were 0.795, 0.205 and 0.739, 0.261 in Xinong Saanen and Guanzhong population respectively. Xinong Saanen breed was in Hardy-Weinberg equilibrium, while Guanzhong breed deviated from Hardy-Weinberg equilibrium (p<0.05). The association of polymorphism with economic traits indicated that the goats with FF genotype have higher milk fat and total solid concentration than those with AA and AF genotypes (p<0.05). Copyright © 2013 by Asian-Australasian Journal of Animal Sciences.

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