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Li T.-P.,Liaoning University | Li T.-P.,Engineering Technology Research Center for Food Bioprocessing of Liaoning Province | Zhu R.-G.,Liaoning University | Zhu R.-G.,Engineering Technology Research Center for Food Bioprocessing of Liaoning Province | And 6 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

The regulatory effects of haw pectin pentaoligosaccharide (HPPS) on fatty acid oxidation-related enzyme activities and mRNA levels were investigated in the liver of high fat diet induced hyperlipidemic mice. Results showed that HPPS (150 mg/kg for 10 weeks) significantly suppresses weight gain (32.3 ± 0.26 and 21.1 ± 0.14 g for high-fat diet and HPPS groups, respectively), decreases serum triacylglycerol levels (1.64 ± 0.09 and 0.91 ± 0.02 mmol/L, respectively), and increases lipid excretion in feces (55.7 ± 0.38 and 106.4 ± 0.57 mg/g for total lipid, respectively), compared to high-fat diet as control. HPPS significantly increased the hepatic fatty acid oxidation-related enzyme activities of acyl-CoA oxidase, carnitine palmitoyltransferase I, 3-ketoacyl-CoA thiolase, and 2,4-dienoyl-CoA reductase by 53.8, 74.2, 47.1, and 24.2%, respectively. Meanwhile, the corresponding mRNAs were up-regulated by 89.6, 85.8, 82.9, and 30.9%, respectively. Moreover, HPPS was able to up-regulate the gene and protein expressions of peroxisome proliferator-activated receptor α. Results suggest that continuous HPPS ingestion may be used as dietary therapy to prevent obesity and cardiovascular diseases. © 2013 American Chemical Society.


Zhu R.,Liaoning University | Li T.,Liaoning University | Dong Y.,Liaoning University | Liu Y.,Liaoning University | And 4 more authors.
Food Research International | Year: 2013

The effects of haw pectin pentasaccharide (HPPS) on hypercholesterolemia were investigated in high-fat fed mice. HPPS significantly decreased the levels of serum total cholesterol (TC) and low density lipoprotein cholesterol (LDL-c), and hepatic TC. It also significantly decreased the activities and gene expressions of 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGCR) and acyl-coenzyme A: cholesterol acyl-transferase (ACAT) in the liver. While, the serum high density lipoprotein cholesterol (HDL-c), fecal bile acids, and the activity and gene expressions of cholesterol 7α-hydroxylase (CYP7A1) were significantly increased by HPPS administration. Moreover, HPPS tended to increase the fecal TC level and to down-regulate the expressions of sterol regulatory element-binding protein 2 (SREBP-2) and LDL receptor in the liver, but no statistical significance was detected. The results revealed that HPPS have great potential in the development of functional food to improve cholesterol metabolism. © 2013 Elsevier Ltd.


Zhu R.-G.,Liaoning University | Sun Y.-D.,Liaoning University | Li T.-P.,Liaoning University | Chen G.,Liaoning Academy of Forestry science | And 7 more authors.
Chemico-Biological Interactions | Year: 2015

This study aims to compare the effects of feeding haw pectin (HP), haw pectin hydrolyzates (HPH), and haw pectin pentasaccharide (HPPS) on the cholesterol metabolism of hypercholesterolemic hamsters induced by high-cholesterol diets. The animals were fed a standard diet (SD), high-cholesterol diet (HCD), or HCD plus HP, HPH, or HPPS at a dose of 300 mg/kg body weight for 4 weeks. Results showed that HPPS was more effective than HP and HPH in decreasing the body weight gain (by 38.2%), liver weight (by 16.4%), and plasma and hepatic total cholesterol (TC; by 23.6% and 27.3%, respectively) of hamsters. In addition, the bile acid levels in the feces were significantly higher by 39.8% and 132.8% in the HPH and HPPS groups than in the HCD group. Such changes were not noted in the HP group. However, the HP group had higher cholesterol excretion capacities than the HPH and HPPS groups by inhibiting cholesterol absorption in the diet, with a 21.7% increase in TC excretion and a 31.1% decrease in TC absorption. Thus, HPPS could be a promising anti-atherogenic dietary ingredient for the development of functional food to improve cholesterol metabolism.


PubMed | Liaoning University and Liaoning Academy of Forestry science
Type: | Journal: Chemico-biological interactions | Year: 2015

This study aims to compare the effects of feeding haw pectin (HP), haw pectin hydrolyzates (HPH), and haw pectin pentasaccharide (HPPS) on the cholesterol metabolism of hypercholesterolemic hamsters induced by high-cholesterol diets. The animals were fed a standard diet (SD), high-cholesterol diet (HCD), or HCD plus HP, HPH, or HPPS at a dose of 300mg/kg body weight for 4weeks. Results showed that HPPS was more effective than HP and HPH in decreasing the body weight gain (by 38.2%), liver weight (by 16.4%), and plasma and hepatic total cholesterol (TC; by 23.6% and 27.3%, respectively) of hamsters. In addition, the bile acid levels in the feces were significantly higher by 39.8% and 132.8% in the HPH and HPPS groups than in the HCD group. Such changes were not noted in the HP group. However, the HP group had higher cholesterol excretion capacities than the HPH and HPPS groups by inhibiting cholesterol absorption in the diet, with a 21.7% increase in TC excretion and a 31.1% decrease in TC absorption. Thus, HPPS could be a promising anti-atherogenic dietary ingredient for the development of functional food to improve cholesterol metabolism.


Chen G.,Liaoning Academy of Forestry science | Xing Z.-K.,Liaoning Academy of Forestry science | Pan W.-L.,Liaoning Academy of Forestry science | Bai L.-P.,ShenYang Agricultural University | And 5 more authors.
African Journal of Biotechnology | Year: 2011

Using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends, a new full-length cDNAs of stearoyl-ACP desturases (SAD) (FaSAD) was obtained from white ash. Sequence analysis showed that the deduced amino acid sequence of FaSAD has high similarity to that of other reported SAD proteins. They are different from each other by some substitutions, insertions and/or deletions involving single amino acid residues or motifs. The analysis of semi-quantitative RTPCR showed that the expression of SAD gene had the highest level in stem and lowest level in leaves. The tertiary structure prediction indicated that FaSAD protein should be a compact globular protein. Based on evolution analysis, it was clear that the genes from the same family were approximately clustered into a group, but all genes from woody plants were not clustered into a separate group. In woody plants, it was indicated that all sequences clustered into two major groups and the FaSAD from white ash was closely related to the SAD gene from Macfadyena unguis-cati.


Han H.,Chinese Academy of Forestry | Sun X.M.,Chinese Academy of Forestry | Xie Y.H.,Chinese Academy of Forestry | Feng J.,Liaoning Academy of Forestry science | Zhang S.G.,Chinese Academy of Forestry
Silvae Genetica | Year: 2013

Adventitious root development is crucial for cutting propagation in hybrid larch. In the present study semilignified cuttings of Larix kaempferi × L. olgensis clones were used to study the morphological and anatomical changes during the different development stages and compare the changes in contents of endogenous phytohormone in two clones with evidently different rooting capacity (25-5 and 23-12), and the effects of exogenous Indole-3-Butyric Acid (IBA) on phytohormone content of a very low native rooting capacity clone (8-9) during the development of adventitious roots. The results showed that the development of adventitious root in semi-lignified cuttings involves three key stages: root cell dedifferentiation and division (14-18 DAC); meristem formation and development (25-35 DAC); root formation and elongation (50-55 DAC). The endogenous phytohormones play a key role during adventitious root development. For 25-5 and 23-12, the level of endogenous phytohormones, especially the ABA content, has an important effect on rooting capacity. For the effects of IBA on 8-9, except lower ABA content, the temporary peak of IAA on the 1 DAC was important for initiating the rooting process. The ratio of (IAA + GA 3+ ZR)/ABA can be used as an indicator of rooting capacity. The anatomical and physiological data described here for semi-lignified cuttings of L. kaempferi × L. olgensis clones improves our knowledge of adventitious roots development. Our study will also benefit future research on examining the molecular mechanisms of the adventitious roots development in hybrid larch.


Feng J.,Chinese Academy of Forestry | Feng J.,Liaoning Academy of Forestry Science | Qi L.-W.,Chinese Academy of Forestry | Sun X.-M.,Chinese Academy of Forestry | And 2 more authors.
Forestry Studies in China | Year: 2010

SSH was used to analyze gene transactivation during root formation of Larix cuttings. Two subtractive cDNA libraries were constructed from clone 31-6 as tester or driver and clone 15-4 as driver or tester. The SSH PCR products from the libraries were cloned into a pGEM-T easy vector and after PCR and dot blot analysis, positive clones were selected, sequenced and compared to the database in GenBank with BLASTX. The results of a sequence assembly in two libraries show that 521 UniEST (expressed sequence tag) were obtained. These 521 UniEST belong to metabolism, signal pathways, transport, resistance, developmental processes, localization, unknown proteins and "no hits found". All of these suggest that subtractive cDNA libraries during root formation of Larix cuttings were constructed successfully. © 2010 Beijing Forestry University and Springer Berlin Heidelberg.


Sun S.-P.,General Station of Forest Pest Management | Luan Q.-S.,Liaoning Academy of Forestry Science | Sheng M.-L.,General Station of Forest Pest Management
Journal of Hymenoptera Research | Year: 2012

A new species, Triclistus strobilius sp. n., belonging to the subfamily Metopiinae (Hymenoptera, Ichneumonidae), reared from Dioryctria pryeri Ragonot, D. rubella Hampson and Gravitarmata margarotana (Hein) in Liaoning, Hunan provinces and Beijing, is reported and described. Illustrations of the new species are provided. © Flávio O. Francisco et al.


Han H.,Chinese Academy of Forestry | Sun X.,Chinese Academy of Forestry | Xie Y.,Chinese Academy of Forestry | Feng J.,Liaoning Academy of Forestry science | Zhang S.,Chinese Academy of Forestry
BMC Plant Biology | Year: 2014

Background: Hybrids of larch (Larix kaempferi × Larix olgensis) are important afforestation species in northeastern China. They are routinely propagated via rooted stem cuttings. Despite the importance of rooting, little is known about the regulation of adventitious root development in larch hybrids. 454 GS FLX Titanium technology represents a new method for characterizing the transcriptomes of non-model species. This method can be used to identify differentially expressed genes, and then two-dimensional difference gel electrophoresis (2D-DIGE) and matrixassisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) analyses can be used to analyze their corresponding proteins. In this study, we analyzed semi-lignified cuttings of two clones of L. kaempferi × L. olgensis with different rooting capacities to study the molecular basis of adventitious root development. Results: We analyzed two clones; clone 25-5, with strong rooting capacity, and clone 23-12, with weak rooting capacity. We constructed four cDNA libraries from 25-5 and 23-12 at two development stages. Sequencing was conducted using the 454 pyrosequencing platform. A total of 957832 raw reads was produced; 95.07% were high-quality reads, and were assembled into 45137 contigs and 61647 singletons. The functions of the unigenes, as indicated by their Gene Ontology annotation, included diverse roles in the molecular functions, biological processes, and cellular component categories. We analyzed 75 protein spots (-fold change ≥2, P ≤ 0.05) by 2D-DIGE, and identified the differentially expressed proteins using MALDI-TOF/TOF MS. A joint analysis of transcriptome and proteome showed genes related to two pathways, polyamine synthesis and stress response, might play an important role on adventitious root development. Conclusions: These results provide fundamental and important information for research on the molecular mechanism of adventitious root development. We also demonstrated for the first time the combined use of two important technologies as a powerful approach to advance research on non-model, but otherwise important, larch species. © 2014 Han et al.

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