Liaoning Academy of Agricultural Science

Dalian, China

Liaoning Academy of Agricultural Science

Dalian, China
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Meng H.,Kunming Medical University | Zhang R.,Kunming Medical University | Yang H.,Yunnan Institute of Parasitic Diseases | Fan Q.,Kunming Medical University | And 5 more authors.
Antimicrobial Agents and Chemotherapy | Year: 2010

Quinine resistance (QNR) in Plasmodium falciparum has been detected in many regions of the world where malaria is endemic. Genetic polymorphisms in at least four genes are implicated in QN susceptibility, and their significance often depends on the genetic background of the parasites. In this study, we have culture-adapted 60 P. falciparum clinical isolates from the China-Myanmar border and assessed their in vitro responses to QN. Our results showed that >50% of the parasite isolates displayed reduced sensitivity to QN, with a half-maximal inhibitory concentration (IC50) above 500 nM. Genotyping of pfcrt found that an overwhelming proportion of the parasite population had the chloroquine-resistant genotype, whereas pfmdr1 mutation genotypes and gene amplification were rare. Genotyping of the P. falciparum Na+/H + exchanger gene (pfnhe1) at the minisatellite ms4760 locus identified 10 haplotypes. Haplotype 7, which harbors three copies of the DNNND repeat, was the most predominant, accounting for nearly half of the parasite isolates. Correlation studies did not reveal significant associations of the polymorphisms in pfcrt and pfmdr1 genes with QN response. However, the ms4760 haplotypes were highly associated with in vitro QN responses. In particular, parasite isolates with an increased DNNND copy number tended to have significantly reduced QN susceptibility, whereas parasite isolates with a higher NHNDNHNNDDD copy number had increased QN susceptibility. This study provided further support for the importance of pfnhe1 polymorphisms in influencing QNR in P. falciparum. Copyright © 2010, American Society for Microbiology. All Rights Reserved.


Grant
Agency: European Commission | Branch: H2020 | Program: RIA | Phase: SFS-13-2015 | Award Amount: 6.43M | Year: 2016

MycoKey aims to generate innovative and integrated solutions that will support stakeholders in effective and sustainable mycotoxin management along food and feed chains. The project will contribute to reduce mycotoxin contamination mainly in Europe and China, where frequent and severe mycotoxin contaminations occur in crops, and where international trade of commodities and contaminated batches are increasing. MycoKey will address the major affected crops maize, wheat and barley, their associated toxigenic fungi and related mycotoxins (aflatoxins, deoxynivalenol, zearalenone, ochratoxin A, fumonisins). The project will integrate key information and practical solutions for mycotoxin management into a smart ICT tool (MycoKey App), providing answers to stakeholders, who require rapid, customized forecasting, descriptive information on contamination risk/levels, decision support and practical economically-sound suggestions for intervention. Tools and methodologies will be strategically targeted for cost-effective application in the field and during storage, processing and transportation. Alternative and safe ways to use contaminated batches will be also delivered. The focus of Mycokey will be: i) innovating communications of mycotoxin management by applying ICT, providing input for legislation, enhancing knowledge and networks; ii) selecting and improving a range of tools for mycotoxin monitoring; iii) assessing the use of reliable solutions, sustainable compounds/green technologies in prevention, intervention and remediation. The multi-disciplinary consortium, composed by scientific, industrial and association partners (32), includes 11 Chinese institutions and will conduct the 4 years programme in a framework of international networks.


De Steur H.,Ghent University | Gellynck X.,Ghent University | Storozhenko S.,Ghent University | Liqun G.,Liaoning Academy of Agricultural science | And 3 more authors.
Appetite | Year: 2010

Neural-tube defects (NTDs) are considered to be the most common congenital malformations. As Shanxi Province, a poor region in the North of China, has one of the highest reported prevalence rates of NTDs in the world, folate fortification of rice is an excellent alternative to low intake of folate acid pills in this region. This paper investigates the relations between socio-demographic indicators, consumer characteristics (knowledge, consumer perceptions on benefits, risks, safety and price), willingness-to-accept and willingness-to-pay genetically modified (GM) rice. The consumer survey compromises 944 face-to-face interviews with rice consumers in Shanxi Province, China. Multivariate analyses consist of multinomial logistic regression and multiple regression. The results indicate that consumers generally are willing-to-accept GM rice, with an acceptance rate of 62.2%. Acceptance is influenced by objective knowledge and consumers' perceptions on benefits and risks. Willingness-to-pay GM rice is influenced by objective knowledge, risk perception and acceptance. Communication towards the use of GM rice should target mainly improving knowledge and consumers' perceptions on high-risk groups within Shanxi Province, in particular low educated women. © 2009 Elsevier Ltd. All rights reserved.


Liu Z.,North Dakota State University | Wang D.,Liaoning Academy of Agricultural science | Feng J.,North Dakota State University | Seiler G.J.,North Dakota State University | And 2 more authors.
Genetics | Year: 2013

The combination of a single cytoplasmic male-sterile (CMS) PET-1 and the corresponding fertility restoration (Rf) gene Rf1 is used for commercial hybrid sunflower (Helianthus annuus L., 2n = 34) seed production worldwide. A new CMS line 514A was recently developed with H. tuberosus cytoplasm. However, 33 maintainers and restorers for CMS PET-1 and 20 additional tester lines failed to restore the fertility of CMS 514A. Here, we report the discovery, characterization, and molecular mapping of a novel Rf gene for CMS 514A derived from an amphiploid (Amp H. angustifolius/P 21, 2n = 68). Progeny analysis of the male-fertile (MF) plants (2n = 35) suggested that this gene, designated Rf6, was located on a single alien chromosome. Genomic in situ hybridization (GISH) indicated that Rf6 was on a chromosome with a small segment translocation on the long arm in the MF progenies (2n = 34). Rf6 was mapped to linkage group (LG) 3 of the sunflower SSR map. Eight markers were identified to be linked to this gene, covering a distance of 10.8 cM. Two markers, ORS13 and ORS1114, were only 1.6 cMaway from the gene. Severe segregation distortions were observed for both the fertility trait and the linked marker loci, suggesting the possibility of a low frequency of recombination or gamete selection in this region. This study discovered a new CMS/Rf gene system derived from wild species and provided significant insight into the genetic basis of this system. This will diversify the germplasm for sunflower breeding and facilitate understanding of the interaction between the cytoplasm and nuclear genes. © 2013 by the Genetics Society of America.


Ni J.,Hangzhou Normal University | Ni J.,Zhejiang University | Zhu Z.,Zhejiang University | Zhu Z.,Liaoning Academy of Agricultural science | And 4 more authors.
PLoS ONE | Year: 2014

The Auxin/Indole-3-Acetic Acid (Aux/IAA) and Auxin Response Factor (ARF) are two important families that play key roles in auxin signal transduction. Both of the families contain a similar carboxyl-terminal domain (Domain III/IV) that facilitates interactions between these two families. In spite of the importance of protein-protein interactions among these transcription factors, the mechanisms involved in these interactions are largely unknown. In this study, we isolated six intragenic suppressors of an auxin insensitive mutant, Osiaa23. Among these suppressors, Osiaa23-R5 successfully rescued all the defects of the mutant. Sequence analysis revealed that an amino acid substitution occurred in the Tryptophan (W) residue in Domain IV of Osiaa23. Yeast two-hybrid experiments showed that the mutation in Domain IV prevents the protein-protein interactions between Osiaa23 and OsARFs. Phylogenetic analysis revealed that the W residue is conserved in both OsIAAs and OsARFs. Next, we performed site-specific amino acid substitutions within Domain IV of OsARFs, and the conserved W in Domain IV was exchanged by Serine (S). The mutated OsARF(WS)s can be released from the inhibition of Osiaa23 and maintain the transcriptional activities. Expression of OsARF(WS)s in Osiaa23 mutant rescued different defects of the mutant. Our results suggest a previously unknown importance of Domain IV in both families and provide an indirect way to investigate functions of OsARFs. Copyright: © 2014 Ni et al.


Zhang F.,Chinese Academy of Agricultural Sciences | Ma X.-F.,Liaoning Academy of Agricultural science | Gao Y.-M.,Chinese Academy of Agricultural Sciences | Hao X.-B.,Liaoning Academy of Agricultural science | Li Z.-K.,Chinese Academy of Agricultural Sciences
BMC Genetics | Year: 2014

Background: Cold stress is an important factor limiting rice yield in many areas of high latitude and altitude. Considerable efforts have been taken to genetically dissect cold tolerance (CT) in rice using DNA markers. Because of possible epistasis and gene × environment interactions associated with identified quantitative trait loci, the results of these genetic studies have unfortunately not been directly applicable to marker-assisted selection for improved rice CT. In this study, we demonstrated the utility of a selective introgression strategy for simultaneous improvement and genetic dissection of rice seedling CT. Results: A set of japonica introgression lines (ILs) with significantly improved seedling CT were developed from four backcross populations based on two rounds of selection. Genetic characterization of these cold-tolerant ILs revealed two important aspects of genome-wide responses to strong phenotypic selection for rice CT: (1) significant over-introgression of donor alleles at 57 loci in 29 functional genetic units (FGUs) across the rice genome and (2) pronounced non-random associations between or among alleles at many unlinked CT loci. Linkage disequilibrium analyses of the detected CT loci allowed us to construct putative genetic networks (multi-locus structures) underlying the seedling CT of rice. Each network consisted of a single FGU, with high introgression as the putative regulator plus two to three groups of highly associated downstream FGUs. A bioinformatics search of rice genomic regions harboring these putative regulators identified a small set of candidate regulatory genes that are known to be involved in plant stress response. Conclusions: Our results suggest that CT in rice is controlled by multiple pathways. Genetic complementarity between parental-derived functional alleles at many loci within a given pathway provides an appropriate explanation for the commonly observed hidden diversity and transgressive segregation of CT and other complex traits in rice. © 2014 Zhang et al.; licensee BioMed Central Ltd.


Wang C.,ShenYang Agricultural University | Wang C.,Liaoning Academy of Agricultural science | Meng X.,ShenYang Agricultural University
Innovative Food Science and Emerging Technologies | Year: 2016

In order to explore the influence rule of 60Co γ-irradiation on preservation effect of blueberry fruit, this research used different doses to deal with “Bluecrop” blueberries. The experimental results indicated that: under the temperature of 0 ± 0.5 °C, the preservation effect of 0.5 kGy irradiation treatment on blueberry fruit was not obvious and 3.0 kGy irradiation treatment can accelerate the ageing process of blueberry fruit in late storage, so 1.0 kGy to 2.5 kGy irradiation treatment were considered to be a feasible method for reducing the fruit rot rate, maintaining fruit firmness and colour, and reducing the loss of nutrients in blueberry fruit during storage, among them, 2.5 kGy irradiation treatment had the best preservation effect on the blueberry fruit stored for 35 days, which fruit decay rate was the lowest (3.35%), fruit firmness was the highest (1.08 N), the degradation of nutrients was the most slowly, the pictures of cell-wall ultrastructure were relatively complete, and the storage period was effectively prolonged to 63 days. Industrial relevance The appropriate doses of irradiation treatment are very efficient in reducing the decay rate, maintaining sensory and nutritional quality, and extending the storage period for blueberry fruit stored at 0 ± 0.5 °C. © 2016 Elsevier Ltd


Yang H.W.,ShenYang Agricultural University | Zhang L.Y.,Liaoning academy of agricultural science | Zhang F.,ShenYang Agricultural University
Advanced Materials Research | Year: 2013

Intelligent materials had been widely used in many fields such as medical, civil construction and aerospace fields, but in agricultural field, there were little applications reported. At present, general PDA equipments could not meet the requirements of data collection as the weakness such as GPS signals were poor, didn't possessed dusk, shockproof, waterproof ability, these problems would be solved easily if these equipments were made based on intelligent materials. In this paper, the basic concept, function and application of intelligent materials were introduced, some key problems such as knowledge base, reasoning machine were explained, at last, the application prospect of intelligent materials were analyzed. © (2013) Trans Tech Publications, Switzerland.


Zhang G.H.,Liaoning Academy of Agricultural science
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2012

The AlHAK1 gene encoding a high-affinity K+ transporter was isolated from Aeluropus littoralis (Gouan) Parl, a graminaceous halophyte, and plays a crucial role in nutrition and ion homeostasis in plant cell. To investigate the regulation role of AlHAK1 on the transcriptional level, an about 1.3 kb 5'-flanking region of the AlHAK1 gene containing a putative promoter was cloned by genome walking method. Cis-regulatory elements analysis showed AlHAK1-promoter region contained typical TATA and CAAT boxes, and some growth and development relative motifs, as well as environmental re-sponsive elements. To reveal the function and regulating role, the AlHAK1 promoter was fused to the β-glucuronidase (GUS) reporter gene in the pCAMBIA1301 vector and introduced into rice via Agrobacterium-mediated transformation. Histo-chemical staining indicated that the GUS expression directed by AlHAK1 promoter was observed in leaves, stems, roots, anther, lemma, and palea. GUS quantitative fluorometric analysis indicated that GUS activity directed by AlHAK1 promoter was lower than CaMV35S and Ubiquitin constitutive promoters; however, in the roots and stems the GUS activity was rela-tively high and displayed a tissue-specific expression pattern. Under ABA, high temperature or drought stress, the GUS activity directed by AlHAK1 promoter was inducible in the roots and stems, suggesting the elements of HSE (-682 bp) and MybBS (-1 268 bp) might play a role in the inducible regulation.


Wang X.Y.,Liaoning Academy of Agricultural science | Zhou L.H.,Liaoning Academy of Agricultural science
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2016

The beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae), is an important and cosmopolitan pest that attacks cultivated crops, including maize, cotton, soybeans, beet, tomato, cabbage, and alfalfa, causing serious economic losses in some of the main crop-producing areas. This species originated in South Asia and is distributed throughout the tropical and temperate regions of Asia, Europe, Africa, and North America. In China, S. exigua was first recorded in Beijing in the 1890s and has been widely distributed in the southern provinces in recent years. However, recently, with global climate warming and the adjustment of agricultural planting structures, S. exigua has quickly spread to the main crop-producing areas of North China. Genetic diversity and population genetic structure are important aspects of the population genetics of agricultural insects, and provide essential information for understanding local adaptation and dispersal patterns, and for clarifying the relationships between genetic variation and outbreaks of pest species. Cytochrome b (Cytb) has a moderate evolutionary rate and a clear evolutionary pattern, suitable for the studies of phylogenetic evolution at the intra- and inter-specific levels. To identify the genetic diversity and population history among geographic populations of S. exigua in North China, and clarify its population demographic history and genetic structure, the genetic diversity, structure, differentiation, and molecular variance were analyzed using DnaSP 5.0 and Arlequin 3.0. In the present study, 798 bases of mtDNA Cytb genes were obtained from 304 individuals of S. exigua, collected from 17 geographic populations in 8 provinces of North China in 2012. Of the 798 characters, 776 sites were conserved and 22 sites were variable (2.8% of the total length), including 9 parsimonious informative sites. The average base frequencies were 42.7% A, 33.1% T, 12.6% G, and 11.5% C. Within-locality diversity was estimated in terms of haplotype diversity (h) and nucleotide diversity (π) from all surveyed localities. Low genetic diversity (Hd = 0.422 ± 0.035,π = 0.00119 ± 0.00011) in the total populations among different geographic populations was detected. The highest estimate (Hd = 0.643 ± 0.106, π = 0.00214 ± 0.00039) was detected in Binzhou (BZ). Nineteen haplotypes, including 9-shared haplotypes were identified. Hap6 was shared in 228 individuals of all populations. Phylogenetic analysis was conducted to determine the relationships between S. exigua haplotypes, and detect discernible groups related to geographic distribution. High congruence was observed between the phylogenies derived from Maximum parsimony (MP), Maximum-likelihood (ML) and Bayesian analyses, and these analyses generated only two inclusive clades. Moreover, the median-joining network was similar to the topology of the phylogenetic tree with 19 haplotypes, and revealed no obvious phylogeographic pattern. The pairwise FST values between the populations varied from -0.049-0.666. Generally, little genetic differentiation (FST = 0.108, P < 0.001) among different geographic populations was detected, with the only significant differentiation between the Lucheng (LUC) and other S. exigua populations. The analysis of molecular variance (AMOVA) showed that the percentage of variation within a population (89.18%) was greater than that between the populations (10.82%). The results of neutrality tests on the S. exigua data set of the total population are: Tajima's D (D = -1.897, P < 0.05) and Fu's Fs (Fs = -4.424, P < 0.05), combined with the unimodal mismatch distribution, indicated recent population expansion of S. exigua in large spatial scales. © 2016, Ecological Society of China. All rights reserved.

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