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Zhao J.-B.,Jinzhou Medical University | Zhao J.-B.,Fushun Oculopathy Hospital | He X.-W.,Liaohe Oil Field General Hospital | Yao S.-Y.,Jinzhou Medical University | And 2 more authors.
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2016

Objective: To explore the function and mechanism of chlorogenic acid (CHA) on human retinoblastoma cell line HXO-RB44 cultured in vitro. Methods: The optic concentration of CHA which inhibited the 6th generation subcultured HXO-RB44 was determined by CCK8 inhibition experiment in vitro. The expressions of anti-oncogene Rb1 and P16, Cyclin D2 and CDK4 were determined by Western blot. The concentration of lactate dehydrogenase (LDH) was detected by enzyme-linked-immunosorbent serologic assay (ELISA). Results: The optimal concentration of CHA for inhibiting HXO-RB44 cell line was 80 μmol/L determined by CCK8 inhibition experiment. The anti-oncogenes Rb1 and P16 were expressed in a low quantity after HXO-RB44 was treated with 80 μmol/L of CHA for 5 days. However, there was hardly Rb1 or P16 expression in the positive group (HT1080) and the normal cultured HXO-RB44 (chlorogenic acid concentration of 0 μmol/L) determined by Western blot methods. The expressions of Cyclin D2 and cyclin dependence kinase 4 (CDK4) were lower after HXO-RB44 received 80 μmol/L of CHA for 5 days than those in the HT1080 cell line and the normal culture HXO-RB44 (P<0.01). The concentration of LDH in medium of HXO-RB44, treated with 80 μmol/L of CHA for 5 days was lower than that of the normal cultured HXO-RB44 [(226.75±42.74)U/L vs. (425.84±31.67)U/L, P<0.01]. Conclusion: CHA of 80 μmol/L may inhibit the proliferation of HXO-RB44 cell line via increasing the anti-oncogene expression and decreasing the expressions of Cyclin D2 and CDK4 as well as the concentration of LDH in medium. © 2016, Editorial Board of Journal of Xi'an Jiaotong University (Medical Sciences). All right reserved.


Zhang D.,Linyi Peoples Hospital | Yang G.,Third Ward of Jining Infectious Disease Hospital | Li X.,Linyi Peoples Hospital | Xu C.,Linyi Peoples Hospital | Ge H.,Liaohe Oil Field General Hospital
Oncology Research | Year: 2016

Cullin7 is an E3 ubiquitin ligase. The Cullin7 protein family functions as a molecular scaffold to coordinate substrate ubiquitination in Skp, Cullin, and F-box-containing complex (SCF complex). Cullin7s control normal development and primary cellular processes and are characterized by a unique genomic network organization. Less is known about the involvement of Cullin7 with hepatocellular carcinoma (HCC). In this study, we found that Cullin7 showed a high expression in HCC tumor tissues, especially in metastatic HCC tumor tissues. Also, there was a negative correlation between Cullin7 expression and long survival. Silencing of Cullin7 in liver cancer cells can significantly reduce the migration, invasion, and metastatic abilities. Also, detection of epithelial- mesenchymal transition (EMT) marker expression showed that Cullin7 promotes epithelial-mesenchymal transformation of cancer cells. The results of this study helped to elucidate the oncogene functions of Cullin7 in liver cancers. © 2016 Cognizant, LLC.


Cao L.,CAS Dalian Institute of Chemical Physics | Cheng L.,Liaohe Oil Field General Hospital | Tang T.,Liaohe Oil Field General Hospital | Liu J.,Liaohe Oil Field General Hospital | And 3 more authors.
Molecular Simulation | Year: 2016

Enhanced sampling is an essential and powerful tool to study complex biochemical processes, notably macromolecular conformational change and chemical reactions. Here we review basic components of some milestones of sampling techniques, focusing especially on the use in understanding enzyme reactions, including reaction path finding, free energy surface construction. © 2016 Informa UK Limited, trading as Taylor & Francis Group.


Huang T.,Liaohe Oil Field General Hospital | Zhao L.,Liaohe Oil Field General Hospital | Zhang L.,Liaohe Oil Field General Hospital | Chen Y.,Liaohe Oil Field General Hospital
Journal of Dalian Medical University | Year: 2015

Objective: To study serum VEGF and IL - 13 levels in asthmatic children and explore the role of VEGF and IL - 13 in pathogenesis of the disease. Methods: Patients were randomly selected including 34 asthmatic children with acute exacerbation (acute exacerbation group), 30 asthmatic children with clinical remission (clinical remission group), and 26 healthy children (control group). Serum VEGF and IL - 13 levels were measured by enzyme - linked immuno sorbent assay (ELISA). Results: The levels of serum VEGF in acute exacerbation group (383. 42 ± 25. 78) pg/mL were significantly higher than those in clinical remission group (177. 95 ±22.46)pg/mL and control group (70. 56 ±18. 94)pg/mL (P < 0.01). The levels of serum VEGF in clinical remission group were significantly higher than those in control group (P < 0.01). The levels of serum IL-13 in acute exacerbation group (56.13 ±9.96)pg/mL were significantly higher than those in clinical remission group (36.72 ±6.29)pg/mL and control group (20.21 ±2. 36)pg/mL (P <0.01). The levels of serum IL-13 in clinical remission group were significantly higher than those in control group (P < 0.01). Conclusion: Elevated serum VEGF and IL-13 may play important roles in the pathogenesis of asthma and can reflect disease severity in asthmatic children.


Zhang Y.,Chinese Academy of Sciences | Yuan X.,Chinese Academy of Sciences | Zhang X.,Chinese Academy of Sciences | Zhang Q.,Chinese Academy of Sciences | And 5 more authors.
Chinese Journal of Cancer Biotherapy | Year: 2015

Objective: To evaluate the effect of a CD20 promoter-driven recombinant adenovirus-soluble trimeric tumor necrosis factor-related apoptosis-inducing ligand (stTRAIL) vector on the growth of lymphoma cells in vitro . Methods: An adenoviral vector encoding stTRAIL driven by the CD20 promoter whose activity was confirmed in lymphoma cell lines by luciferase assay was constructed. The constructed vector was infected into CD20 positive and CD20 negative lymphoma cell lines respectively. Protein content of stTRAIL was assessed by Western blotting and cell viability was determined by MTT assay in infected cells. Results: Soluble trimeric TRAIL protein was detected in CD20 positive lymphoma cells but not in CD20 negative lymphoma cells after infection with AdP20-stTRAIL ( P<0.05). Overexpression of stTRAIL in BJAB cells resulted in a significant increase in apoptotic cell death ( P<0.05). Conclusion: The CD20 promotor is capable of enhancing stTRAIL transcription in CD20 positive lymphoma cells, thereby having significant clinical implications in targeted cancer therapy. © 2015, Editorial office of Chinese Journal of Cancer Biotherapy. All rights reserved.

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