Lianyungang TCM Branch of Jiangsu Union Technical Institute

Xinpu, China

Lianyungang TCM Branch of Jiangsu Union Technical Institute

Xinpu, China
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Li R.,Hebei University of Technology | Fu N.,Hebei University | Wu Z.,Hebei University of Technology | Wang Y.,Hebei University of Technology | And 2 more authors.
Chemical Engineering Research and Design | Year: 2016

Improving foam stability is an important issue in foam fractionation of proteins from their aqueous solutions with low foam properties. In this work, enhancing protein self-association at the gas-liquid interface by sodium citrate (Na-citrate), instead of adding surfactants, was used to improve the stability of protein foams. Using bovine serum albumin (BSA) as a model protein, the role of Na-citrate in enhancing self-association of BSA at the gas-liquid interface was studied at the molecular level. Then, the role of the enhanced protein self-association in intensifying foam fractionation of BSA was studied. The results show that by weakening electrostatic repulsion between the BSA molecules and unfolding their structures, Na-citrate induced the formation of 12 and 18 BSA aggregates at the gas-liquid interface. The enhanced protein self-association effectively increased the interfacial adsorption of BSA and improved the foam stability. At BSA concentration 50 mg/L, the recovery percentage of BSA with 30 mmol/L Na-citrate increased by 4 folds while its enrichment ratio just decreased by 11.5%, compared to those without Na-citrate. The results give a clear understanding of the role of protein self-association in foam fractionation of proteins. © 2016 The Institution of Chemical Engineers.


Han T.,Huazhong Agricultural University | Cheng G.,South-Central University for Nationalities | Liu Y.,Huazhong Agricultural University | Yang H.,Huazhong Agricultural University | And 2 more authors.
Food and Chemical Toxicology | Year: 2012

Tectoridin (4',5,7-thrihydroxy-6-methoxyisoflavone-7-O-β-d-glucopyranoside) isolated from the flowers of Pueraria thunbergiana is reported to have less hepatoprotective, hypoglycemic, antiallergic and anaphylaxis inhibitory activity than its aglycone form tectorigenin. To obtain tectorigenin, tectoridin was hydrolyzed in the current study. However, practical limitations of tectorigenin do exist due to its poor water-solubility. To increase its water-solubility, tectorigenin was sulfonated with sulfuric acid (98wt.%) and mixed with saturated salt water to produce tectorigenin sodium sulfonate. Tectoridin and the two transfer products were identified by UV, IR, HPLC-MS, 1H NMR and 13C NMR, and the solubility of tectorigenin sodium sulfonate was increased about 9-fold than tectorigenin. Antioxidant experiments of tectoridin, tectorigenin and modified tectorigenin in vitro including reducing power, superoxide anion radical scavenging activity, hydroxyl radical scavenging activity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity and anti-lipid peroxidation were carried on comparing with ascorbic acid (Vc) or butylated hydroxytoluene (BHT). The results suggested that the antioxidant activity in all the experimental systems exhibited the same order as follows: tectorigenin sodium sulfonate>tectorigenin>tectoridin. Due to the high water-solubility and good antioxidant properties with tectorigenin sodium sulfonate, appropriate chemical modifications could greatly improve the biological activities of the naturally occurring products. © 2011 Elsevier Ltd.


Li R.,Hebei University of Technology | Wu Z.,Hebei University of Technology | Wang Y.,Hebei University of Technology | Ding L.,Hebei University of Technology | Wang Y.,Lianyungang TCM Branch of Jiangsu Union Technical Institute
Journal of Biotechnology | Year: 2016

In this work, β-cyclodextrin was developed to prevent protein aggregation in foam fractionation using bovine serum albumin (BSA) as a model protein. The role of β-cyclodextrin in preventing the aggregation of BSA induced by the gas-liquid interface was studied at the molecular level. The results indicate that by holding the exposed phenylalanine residues in its hydrophobic cavity, β-cyclodextrin effectively prevented the aggregation of BSA induced by the gas-liquid interface in foam fractionation. Furthermore, β-cyclodextrin could be effectively separated from BSA in the foamate due to their weak association. © 2016 Elsevier B.V.


Li R.,Hebei University of Technology | Wu Z.,Hebei University of Technology | Wangb Y.,Hebei University of Technology | Ding L.,Hebei University of Technology | Wang Y.,Lianyungang TCM Branch of Jiangsu Union Technical Institute
Biotechnology Reports | Year: 2016

For reducing protein aggregation in foam fractionation, the role of pH-induced structural change in the interface-induced protein aggregation was analyzed using bovine serum albumin (BSA) as a model protein. The results show that the decrease in pH from 7.0 to 3.0 gradually unfolded the BSA structure to increase the molecular size and the relative content of β-sheet and thus reduced the stability of BSA in the aqueous solution. At the isoelectric point (pH 4.7), BSA suffered the lowest level in protein aggregation induced by the gas-liquid interface. In the pH range from 7.0 to 4.7, most BSA aggregates were formed in the defoaming process while in the pH range from 4.7 to 3.0, the BSA aggregates were formed at the gas-liquid interface due to the unfolded BSA structure and they further aggregated to form insoluble ones in the desorption process. © 2016 The Author.


Li R.,Hebei University of Technology | Li R.,Jining Medical University | Ding L.,Hebei University of Technology | Wu Z.,Hebei University of Technology | And 3 more authors.
Industrial Crops and Products | Year: 2016

For reducing the costs of separating bromelain from its crude extract, a two-stage foam fractionation technology with β-cyclodextrin as an activity protector was developed using the foam fractionation column with vertical sieve trays (VSTs). By studying the effects of VSTs, volumetric air flowrate, β-cyclodextrin concentration, aeration time and temperature, the two-stage foam fractionation technology were optimized. The results show that VSTs effectively improved the activity recovery by enhancing the adsorption of bromelain at the gas-liquid interface while β-cyclodextrin significantly reduced the activity loss induced by the gas-liquid interface. At β-cyclodextrin concentration 0.60 mg/mL, a high specific activity of 165.6 U/mg, 7.4 folds of that in the crude extract, was obtained with an activity recovery of 45.2% by using the optimized two-stage foam fractionation technology. Compared to those without β-cyclodextrin, activity recovery and specific activity obtained satisfactory relative increases of 34.1% and 110.7%, respectively. © 2016 Elsevier B.V.


Mu L.-N.,CAS Xinjiang Technical Institute of Physics and Chemistry | Mu L.-N.,Lianyungang TCM Branch of Jiangsu Union Technical Institute | Wei Z.-H.,CAS Xinjiang Technical Institute of Physics and Chemistry | Wei Z.-H.,Lianyungang TCM Branch of Jiangsu Union Technical Institute | Liu Z.-S.,CAS Xinjiang Technical Institute of Physics and Chemistry
Electrophoresis | Year: 2015

This review focused on the developments in the field of molecularly imprinted polymers (MIPs) for CEC since 2009. New preparation techniques of MIP-based CEC, such as, portable microchip with macroporous monolithic imprinted microchannel, and low cross-linking MIPs based on liquid crystalline monomers, were discussed. Using selected cases rather than a comprehensive review of the entire field, our goal is to highlight the studies of the interest with an emphasis on recent work, and offers suggestions for future development in the field of imprinted materials for CEC separation. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Liang D.,Yaan Vocational College | Wang Y.,Yaan Vocational College | Wang Y.,Lianyungang TCM Branch of Jiangsu Union Technical Institute | Di D.,Shenyang Pharmaceutical University
Journal of Chemical Research | Year: 2015

A facile synthesis of 4,5,5-trimethyl-5,6-dihydrobenzo[c][2,7]naphthyridine, the debrominated analogue of the marine alkaloid veranamine, has been achieved in three steps with a 38% overall yield. from the commercially available 2-bromoaniline. The key benzo[c][2,7]naphthyridine moiety was constructed using a Sonogashira coupling, a tandem Rupe rearrangement-Donnelly-Farrell cyclisation and a Diels-Alder reaction as the key steps. The synthetic strategy allows rapid access to various analogues of veranamine.


Li R.,Hebei University of Technology | Fu N.,Hebei University of Technology | Fu N.,Hebei University | Wu Z.,Hebei University of Technology | And 2 more authors.
Biochemical Engineering Journal | Year: 2015

In foam fractionation of proteins, a clear understanding of their aggregation induced by the gas-liquid interface has great importance to reducing the loss of their bioactivity. In this work, the effects of the concentration of bovine serum albumin (BSA) on its aggregation induced by the gas-liquid interface were investigated at the molecular level to clearly explain how this protein aggregated in foam fractionation. The results show that reducing the BSA concentration made the protein structure more unfolded so that it enhanced the protein aggregation induced by the gas-liquid interface. In foam fractionation, the BSA aggregates were mainly formed in the desorption of the BSA molecules from the gas-liquid interface. Their formation was closely related to the BSA enrichment ratio. In the concentration from 0.05. g/L to 0.20. g/L, the highly unfolded BSA structure and the high enrichment jointly resulted in the formation of insoluble aggregates. © 2015 Elsevier B.V.


PubMed | Lianyungang TCM Branch of Jiangsu Union Technical Institute and Hebei University of Technology
Type: | Journal: Journal of biotechnology | Year: 2016

In this work, -cyclodextrin was developed to prevent protein aggregation in foam fractionation using bovine serum albumin (BSA) as a model protein. The role of -cyclodextrin in preventing the aggregation of BSA induced by the gas-liquid interface was studied at the molecular level. The results indicate that by holding the exposed phenylalanine residues in its hydrophobic cavity, -cyclodextrin effectively prevented the aggregation of BSA induced by the gas-liquid interface in foam fractionation. Furthermore, -cyclodextrin could be effectively separated from BSA in the foamate due to their weak association.


PubMed | Lianyungang TCM Branch of Jiangsu Union Technical Institute and Nanjing University
Type: Journal Article | Journal: BMC complementary and alternative medicine | Year: 2016

Tripterygium wilfordii Radix, a well-known traditional medicine in china which is used for treatment of inflammation, pain, tumor and immune regulation for centuries in china, accompany with the serious toxic side effects. This study was carried out for simultaneously analyzing the four main components (triptolide, triptophenolide, demethylzeylasteral and celastrol) in Tripterygium wilfordii Radix under different drying processes, which was important for reducing the toxicity and quality control of Tripterygium wilfordii Radix in future.The terpenes were extracted by using ultrasonic method with ethyl acetate from root or cortex of Tripterygium wilfordii Radix, and the sensitive and rapid HPLC-PDA method was developed for simultaneous quantification of triptolide, triptophenolide, demethylzeylasteral and celastrol in root and cortex of Tripterygium wilfordii Radix for evaluation of the impacts by different drying processes.The four compounds in their respective determined arrange had good linearity of 0.9998RThe method established is high sensitivity, accuracy, reliability and suitable for the simultaneous analysis of terpenoids in Tripterygium wilfordii Radix. The data provide a scientific basis and reference for the quality control of herb and preparations related to Tripterygium wilfordii Radix.

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