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Zuo X.-F.,Lianshui County Peoples Hospital | Li J.-X.,Nanjing Medical University | Zhou W.-D.,Nanjing Medical University
Biomedical Research (India) | Year: 2014

Recent studies have shown that zinc, iron, vitamin A and other nutrients are closely related to the body's resistance to infection. This study was aimed to investigate the nutrient status of Chinese infants with pneumonia and reveal the relationship between nutrients and the outcome of pneumonia. Total 120 infants with pneumonia and 60 healthy infants aged 6 to 36 months were enrolled in this study as diseased and control groups. Serum levels of zinc, calcium, magnesium, iron, vitamin A and vitamin D were measured, and the values were compared between the two groups. We found that the incidences of iron, zinc, and vitamin A deficiency were significantly higher in diseased group than in control group (p<0.05), while the incidences of calcium, magnesium and vitamin D deficiency had no significant differences (p>0.05). Serum levels of zinc, iron, and vitamin A were significantly lower in diseased group than in control group (p<0.05), while those of calcium, magnesium and vitamin D had no significant difference (p>0.05). In conclusion, Zinc, iron, and vitamin A deficiency were observed among Chinese children with pneumonia. Zinc, iron and vitamin A supplementation may be helpful for the prevention and treatment of infant pneumonia. Source


Zhang X.-S.,Lianshui County Peoples Hospital | Zhao Z.-Q.,Huaian Second Peoples Hospital | Qin Z.-S.,Nanjing Medical University | Wu K.,Nanjing Medical University | And 2 more authors.
European Journal of Drug Metabolism and Pharmacokinetics | Year: 2015

Herb-drug interaction strongly limits the clinical utilization of herbs and drugs. Irinotecan-induced diarrhea is closely related with the UDP-glucuronosyltransferase 1A1-catalyzed glucuronidation of SN-38 which has been widely regarded to be the toxic substance basis of irinotecan. The present study aims to determine the influence of herbal component psoralidin toward the toxicity of irinotecan. In vitro inhibition potential of psoralidin toward the glucuronidation of SN-38 was firstly investigated using human intestinal microsomes incubation system. Dose-dependent inhibition of psoralidin toward SN-38 glucuronidation was observed. Furthermore, Dixon plot showed that the intersection point was located in the second quadrant, indicating the competitive inhibition of psoralidin toward the glucuronidation of SN-38. Through the data fitting using competitive inhibition fitting equation, the inhibition kinetic parameter (K i) was calculated to be 5.8 μM. The translation of these in vitro data into the in vivo situation showed that pre-treatment with psoralidin significantly increased the toxicity of irinotecan, as indicated by the increased body weight loss and more severe colon histology damage. All these data indicated the herb-drug interaction between irinotecan and psoralidin-containing herbs. © 2014 Springer International Publishing Switzerland. Source


Chen G.,Lianshui County Peoples Hospital | Peng J.,Nanjing Medical University | Zhu W.,Nanjing Medical University | Tao G.,Nanjing Medical University | And 3 more authors.
Medical Oncology | Year: 2014

microRNA-133a (miR-133a) and miR-133b, located on chromosome 18 in the same bicistronic unit, have been commonly identified as being downregulated in esophageal squamous cell carcinoma (ESCC). The aim of this study was to investigate the correlation of miR-133a/b expression with efficacy of paclitaxel-based chemotherapy and clinical outcome of ESCC patients. miR-133a expression and miR-133b expression were examined in 100 newly diagnosed ESCC patients prior to treatment by quantitative real-time PCR. Then, the patients received four cycles of paclitaxel-based chemotherapy, the short-term treatment efficacy was evaluated, and a 3-year follow-up was performed. Expression levels of miR-133a and miR-133b were both significantly lower in ESCC tissues compared to adjacent noncancerous tissues (both P < 0.001). In addition, combined miR-133a/b downregulation was found to be closely correlated with advanced tumor stage (P = 0.02) and poor differentiation (P = 0.01). Moreover, the response rate of ESCC patients to paclitaxel-based chemotherapy was significantly higher in combined miR-133a/b downregulation group compared with other groups (P = 0.02). Furthermore, univariate and multivariate Cox analyses revealed that tumor stage and combined expression of miR-133a/b were independent prognosis factors in ESCC patients. Our data offer the convincing evidence that combined expression of miR-133a and miR-133b may predict chemosensitivity of patients with ESCC undergoing paclitaxel-based chemotherapy, implying its importance in applying ‘personalized cancer medicine’ in the clinical treatment of ESCC. We also identified combined expression of miR-133a and miR-133b as an effective prognostic marker of this malignancy. © 2014, Springer Science+Business Media New York. Source


Chen G.,Lianshui County Peoples Hospital | Peng J.,Lianshui County Peoples Hospital | Zhu W.,Lianshui County Peoples Hospital | Tao G.,Lianshui County Peoples Hospital | And 3 more authors.
Medical oncology (Northwood, London, England) | Year: 2014

microRNA-133a (miR-133a) and miR-133b, located on chromosome 18 in the same bicistronic unit, have been commonly identified as being downregulated in esophageal squamous cell carcinoma (ESCC). The aim of this study was to investigate the correlation of miR-133a/b expression with efficacy of paclitaxel-based chemotherapy and clinical outcome of ESCC patients. miR-133a expression and miR-133b expression were examined in 100 newly diagnosed ESCC patients prior to treatment by quantitative real-time PCR. Then, the patients received four cycles of paclitaxel-based chemotherapy, the short-term treatment efficacy was evaluated, and a 3-year follow-up was performed. Expression levels of miR-133a and miR-133b were both significantly lower in ESCC tissues compared to adjacent noncancerous tissues (both P < 0.001). In addition, combined miR-133a/b downregulation was found to be closely correlated with advanced tumor stage (P = 0.02) and poor differentiation (P = 0.01). Moreover, the response rate of ESCC patients to paclitaxel-based chemotherapy was significantly higher in combined miR-133a/b downregulation group compared with other groups (P = 0.02). Furthermore, univariate and multivariate Cox analyses revealed that tumor stage and combined expression of miR-133a/b were independent prognosis factors in ESCC patients. Our data offer the convincing evidence that combined expression of miR-133a and miR-133b may predict chemosensitivity of patients with ESCC undergoing paclitaxel-based chemotherapy, implying its importance in applying 'personalized cancer medicine' in the clinical treatment of ESCC. We also identified combined expression of miR-133a and miR-133b as an effective prognostic marker of this malignancy. Source


Qing Z.S.,Nanjing Medical University | Zhang X.S.,Lianshui County Peoples Hospital | Gao C.C.,Nanjing Medical University | Liu W.D.,Nanjing Medical University | And 3 more authors.
Genetics and Molecular Research | Year: 2015

We explored the protective effect of ischemia preconditioning (IP) on ischemia-reperfusion injury in rat liver transplantation. An orthotopic liver transplantation model was utilized in the study. A total of 54 Sprague-Dawley rats were divided into a control group (group A, no liver transplantation), liver transplantation group (group B, heparin Ringer’s lactate solution was perfused via the portal vein before donor liver collection), and liver transplantation with IP group (group C, IP was performed for different time periods before donor liver collection). Liver function, B-cell lymphoma 2 expression in hepatic cells, cell apoptosis, and cellular ultrastructure changes were detected after surgery. After surgery, serum alanine aminotransferase activity was significantly higher in group B than in group A, while it was not clearly enhanced in group C and decreased progressively with increasing cycles of IP as bile capacity gradually increased. Compared with group B, group C showed alleviated injury of hepatic cells, increased B-cell lymphoma 2 expression, and a lower apoptosis index. IP had a protective effect on ischemia-reperfusion injury in rat liver transplantation, and the mechanism correlated with increased B-cell lymphoma 2 expression in hepatic cells and inhibition of cell apoptosis. © FUNPEC-RP. Source

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